FastQCFastQC Report
Thu 9 Feb 2017
SRR2727247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4916216
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT624701.2706927441756017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238040.48419353421411915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223960.4555536209149476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112210.22824464995028695No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG87090.17714844099608315No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG82200.16720176656192487No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79970.16266575756638846No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG79470.16164871519070764No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT76030.1546514636460237No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG75180.15292249160736632No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG67720.13774821936220866No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA65660.13355800477440372No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT63200.12855415628605416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG6350.018.645671
AAGTACG12300.018.048781
TATACCG3250.016.5076925
AATCAAC17100.015.0380121
TCTATAC4600.014.4782613
GAGTACT190000.014.42999911
GTACTTT190950.014.37758513
AGTACTT193450.014.18221812
TACTTTT197000.013.98299614
CTATACT5850.013.9145294
ACTTTTT211550.013.23115215
CTACACT9800.012.6479594
CGTACAC2951.8189894E-1212.5423743
CTTTTTT224350.012.50100316
TAGACTG6100.012.4344265
TATGCGC1653.810117E-612.33333310
GTATACT3200.012.1406264
TATACTG5650.012.1150455
TACTCCG3750.011.8399995
TACACTG21950.011.7152625
Produced by FastQC (version 0.11.5)