##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727247.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4916216
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.89950644967593	31.0	30.0	33.0	25.0	34.0
2	30.25252633326119	31.0	30.0	34.0	26.0	34.0
3	31.072921328110887	31.0	30.0	34.0	28.0	34.0
4	35.069849453319385	35.0	35.0	37.0	32.0	37.0
5	34.91874848460686	35.0	35.0	37.0	32.0	37.0
6	34.97498787685488	35.0	35.0	37.0	32.0	37.0
7	34.83443078985952	35.0	35.0	37.0	32.0	37.0
8	34.834424077379836	35.0	35.0	37.0	32.0	37.0
9	36.24080288579672	38.0	35.0	39.0	32.0	39.0
10	36.01002457988014	37.0	35.0	39.0	30.0	39.0
11	36.09272924541965	37.0	35.0	39.0	31.0	39.0
12	35.882973001999915	37.0	35.0	39.0	30.0	39.0
13	35.909058715076796	37.0	35.0	39.0	30.0	39.0
14	36.67399337213825	38.0	35.0	40.0	30.0	41.0
15	36.69130567086556	38.0	36.0	40.0	30.0	41.0
16	36.47457821218596	38.0	35.0	40.0	30.0	41.0
17	36.99512124772386	38.0	36.0	40.0	31.0	41.0
18	37.18539034900012	39.0	36.0	40.0	31.0	41.0
19	37.3276015537153	39.0	36.0	40.0	32.0	41.0
20	37.37148082997167	39.0	36.0	41.0	32.0	41.0
21	37.32950179569002	39.0	36.0	41.0	31.0	41.0
22	37.29047787973514	39.0	36.0	41.0	31.0	41.0
23	37.17378569208513	39.0	36.0	40.0	31.0	41.0
24	37.07092894209693	39.0	36.0	40.0	31.0	41.0
25	36.96286534196219	39.0	36.0	40.0	31.0	41.0
26	36.67053705532873	39.0	36.0	40.0	30.0	41.0
27	36.501615470109535	39.0	35.0	40.0	30.0	41.0
28	36.32296038253811	39.0	35.0	40.0	30.0	41.0
29	36.115385491605736	38.0	35.0	40.0	29.0	41.0
30	35.96360879993882	38.0	35.0	40.0	29.0	41.0
31	35.759622441324794	38.0	34.0	40.0	27.0	41.0
32	35.57726206497029	38.0	34.0	40.0	27.0	41.0
33	35.4273184904813	38.0	34.0	40.0	27.0	41.0
34	35.279442359733586	38.0	34.0	40.0	26.0	41.0
35	35.09117764557131	38.0	34.0	40.0	25.0	41.0
36	34.93352895804416	38.0	33.0	40.0	25.0	41.0
37	34.77204317304203	38.0	33.0	40.0	25.0	41.0
38	34.65746541649106	38.0	33.0	40.0	24.0	41.0
39	34.52149620765239	38.0	33.0	40.0	24.0	41.0
40	34.38043243014546	38.0	33.0	40.0	23.0	41.0
41	34.215015776361334	38.0	33.0	40.0	23.0	41.0
42	34.006145580259286	37.0	33.0	40.0	21.0	41.0
43	33.633089555056166	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	7.0
13	22.0
14	43.0
15	91.0
16	183.0
17	374.0
18	853.0
19	1886.0
20	3809.0
21	7138.0
22	12667.0
23	20170.0
24	30064.0
25	43044.0
26	58462.0
27	77667.0
28	101120.0
29	129560.0
30	162911.0
31	203039.0
32	248240.0
33	302869.0
34	365931.0
35	438159.0
36	540354.0
37	673837.0
38	773841.0
39	719867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.421084020718375	28.386629065932013	14.721668047132185	21.470618866217432
2	19.56750883199599	26.787451975259017	30.01249334854286	23.632545844202127
3	20.326730965441715	25.44739694106199	30.51175945076457	23.714112642731727
4	15.907824229041198	17.296412525405717	31.770044277956867	35.02571896759621
5	13.798274933404064	40.06746245486366	32.272910710188484	13.86135190154379
6	34.62756721836469	37.63372073155452	12.20603407173322	15.532677978347575
7	28.76214145188088	31.563971151796423	19.81483319691405	19.85905419940865
8	29.387337741059383	35.93721268552887	17.401961183153873	17.273488390257874
9	26.898187549123147	15.153117763743499	18.111734716293995	39.83695997083936
10	16.850297057737087	30.59121080115276	31.834117947624758	20.724374193485396
11	35.421368792583564	24.50982625661688	18.624954639910044	21.443850310889516
12	21.114186195236336	27.987684023647457	28.530418517005764	22.367711264110447
13	32.321199882185816	20.46734724430334	23.089282488808465	24.122170384702383
14	21.78779370149725	22.847145040006378	25.7807020684201	29.58435919007627
15	25.487834545919057	29.80845430713378	21.890087823643224	22.81362332330394
16	23.376413892310673	27.057029227356978	24.685327088964357	24.881229791367996
17	23.78719730784815	27.985263462793338	25.923820271525905	22.30371895783261
18	23.301783322783216	26.26029857109614	26.80801657209529	23.629901534025354
19	23.35615440818711	27.82465213082582	27.373593837211384	21.445599623775685
20	23.218975732555283	27.27378129846207	26.885169406714432	22.622073562268216
21	22.339844303016793	28.239666442646133	26.755659230595235	22.664830023741835
22	23.168428726483945	27.835981982890907	25.852708668618302	23.142880622006846
23	22.80514118989076	27.403555905598942	25.994484375788208	23.796818528722092
24	22.892830583521963	27.768979231181056	26.24660918071948	23.091581004577506
25	23.42041114548262	26.816274956185815	25.94340850768152	23.819905390650046
26	23.256748686388068	27.366962720921943	26.776447576754155	22.599841015935834
27	23.737016437032057	27.800141409571914	26.33712595215507	22.125716201240955
28	22.936624428218778	28.47425743702067	26.475423374400147	22.11369476036041
29	23.018557362003623	28.385530660166275	26.62132827361532	21.974583704214787
30	22.45885860181896	28.48412274806477	26.671610848668976	22.38540780144729
31	22.76205927485692	27.71119088339487	26.67106164578611	22.8556881959621
32	22.043722244913567	28.067643895223483	26.40856300862289	23.480070851240058
33	22.4151461205122	28.40770218395612	26.20165590771439	22.975495787817295
34	23.068494142649552	28.1287681420019	26.05745963969036	22.74527807565819
35	22.68948313092834	28.354612571945577	26.54834531273646	22.40755898438962
36	22.855830581894693	28.53214748904442	26.244656459358172	22.367365469702715
37	22.17546991425926	28.066565830305258	26.81373235024661	22.944231905188868
38	22.56973656161568	28.403979808861124	26.742132566998684	22.28415106252451
39	22.058713449531105	28.391307460860144	26.538500342539873	23.011478747068885
40	22.718895996433027	28.284843464973875	27.173155125812208	21.823105412780887
41	23.051184081415464	27.655456961207562	26.72618534254801	22.567173614828967
42	21.98711366628317	28.252826970987442	27.179501470236456	22.580557892492926
43	22.831360542335812	27.41390939698337	26.950483868080653	22.80424619260016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3198.0
1	2833.0
2	2468.0
3	2398.5
4	2329.0
5	2329.0
6	2717.0
7	3105.0
8	4398.0
9	5691.0
10	8954.5
11	12218.0
12	12218.0
13	33693.5
14	55169.0
15	88934.0
16	122699.0
17	91533.5
18	60368.0
19	60368.0
20	68907.0
21	77446.0
22	63177.5
23	48909.0
24	53949.5
25	58990.0
26	58990.0
27	71069.0
28	83148.0
29	93855.5
30	104563.0
31	118839.5
32	133116.0
33	133116.0
34	144637.5
35	156159.0
36	174323.0
37	192487.0
38	212464.5
39	232442.0
40	232442.0
41	253124.0
42	273806.0
43	291052.0
44	308298.0
45	329454.0
46	350610.0
47	350610.0
48	377489.5
49	404369.0
50	419147.5
51	433926.0
52	434303.0
53	434680.0
54	434680.0
55	401783.0
56	368886.0
57	322440.0
58	275994.0
59	249342.0
60	222690.0
61	222690.0
62	193722.5
63	164755.0
64	139167.5
65	113580.0
66	94779.0
67	75978.0
68	75978.0
69	62441.5
70	48905.0
71	40504.5
72	32104.0
73	26202.0
74	20300.0
75	20300.0
76	16584.0
77	12868.0
78	10616.0
79	8364.0
80	6650.0
81	4936.0
82	4936.0
83	3902.0
84	2868.0
85	2294.5
86	1721.0
87	1375.5
88	1030.0
89	1030.0
90	770.5
91	511.0
92	400.5
93	290.0
94	217.5
95	145.0
96	145.0
97	99.0
98	53.0
99	48.5
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4916216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.5659626572668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34271891969065	46.57795163509037
2	9.33539566612181	10.561312852813135
3	3.104275225619501	5.267889494708135
4	1.5511055506904015	3.5095911463132996
5	0.8459770105102907	2.392675199271565
6	0.5719774895844673	1.941267438997931
7	0.3993521029773409	1.5812815300882064
8	0.2925056777102133	1.3236692193915556
9	0.2310057454405102	1.1760356133181782
>10	1.2220732830286598	12.474425696204
>50	0.07035259298163027	2.699769359696907
>100	0.027337405261682778	2.773472030589641
>500	0.0031615332784596605	1.2263979690766376
>1k	0.002289384441919203	2.639173498981191
>5k	3.270549202737672E-4	1.3906377047687368
>10k+	1.4535774234389654E-4	2.464449610690425
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	62470	1.2706927441756017	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23804	0.48419353421411915	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22396	0.4555536209149476	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11221	0.22824464995028695	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	8709	0.17714844099608315	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	8220	0.16720176656192487	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7997	0.16266575756638846	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	7947	0.16164871519070764	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	7603	0.1546514636460237	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	7518	0.15292249160736632	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	6772	0.13774821936220866	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	6566	0.13355800477440372	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6320	0.12855415628605416	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	2.0340847513616163E-5	0.0	0.0	0.0	0.0
4	2.0340847513616163E-5	0.0	0.0	0.0	2.0340847513616163E-5
5	2.0340847513616163E-5	0.0	0.0	0.0	2.0340847513616163E-5
6	2.0340847513616163E-5	0.0	0.0	0.0	6.102254254084849E-5
7	2.0340847513616163E-5	0.0	0.0	0.0	6.102254254084849E-5
8	2.0340847513616163E-5	0.0	0.0	0.0	8.136339005446465E-5
9	2.0340847513616163E-5	0.0	0.0	0.0	2.4409017016339396E-4
10	2.0340847513616163E-5	0.0	0.0	6.102254254084849E-5	2.4409017016339396E-4
11	2.0340847513616163E-5	0.0	0.0	8.136339005446465E-5	2.4409017016339396E-4
12	2.0340847513616163E-5	0.0	0.0	8.136339005446465E-5	2.4409017016339396E-4
13	2.0340847513616163E-5	2.0340847513616163E-5	0.0	8.136339005446465E-5	2.644310176770101E-4
14	2.0340847513616163E-5	4.0681695027232326E-5	0.0	1.0170423756808082E-4	4.6783949281317173E-4
15	2.0340847513616163E-5	6.102254254084849E-5	0.0	1.627267801089293E-4	4.6783949281317173E-4
16	6.102254254084849E-5	6.102254254084849E-5	0.0	2.237493226497778E-4	5.898845778948687E-4
17	6.102254254084849E-5	6.102254254084849E-5	0.0	4.068169502723233E-4	5.898845778948687E-4
18	6.102254254084849E-5	6.102254254084849E-5	0.0	4.881803403267879E-4	6.712479679493333E-4
19	6.102254254084849E-5	6.102254254084849E-5	0.0	7.526113580037981E-4	7.119296629765657E-4
20	6.102254254084849E-5	6.102254254084849E-5	0.0	0.0012611325458442022	7.119296629765657E-4
21	6.102254254084849E-5	6.102254254084849E-5	0.0	0.0024205608541203235	7.526113580037981E-4
22	6.102254254084849E-5	6.102254254084849E-5	0.0	0.00376305679001899	7.526113580037981E-4
23	6.102254254084849E-5	6.102254254084849E-5	0.0	0.0047597583181861825	9.763606806535758E-4
24	6.102254254084849E-5	6.102254254084849E-5	0.0	0.006814183917061414	0.0010170423756808082
25	8.136339005446465E-5	8.136339005446465E-5	0.0	0.007973612225337537	0.0010577240707080405
26	8.136339005446465E-5	8.136339005446465E-5	0.0	0.00980428850156299	0.0010984057657352727
27	8.136339005446465E-5	8.136339005446465E-5	0.0	0.013669049529150061	0.0010984057657352727
28	8.136339005446465E-5	8.136339005446465E-5	0.0	0.02141891243183782	0.0010984057657352727
29	8.136339005446465E-5	8.136339005446465E-5	0.0	0.039827379431660447	0.0010984057657352727
30	8.136339005446465E-5	8.136339005446465E-5	0.0	0.06496866695849003	0.0010984057657352727
31	8.136339005446465E-5	8.136339005446465E-5	0.0	0.12857449713356778	0.0011390874607625052
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGG	635	0.0	18.64567	1
AAGTACG	1230	0.0	18.04878	1
TATACCG	325	0.0	16.507692	5
AATCAAC	1710	0.0	15.038012	1
TCTATAC	460	0.0	14.478261	3
GAGTACT	19000	0.0	14.429999	11
GTACTTT	19095	0.0	14.377585	13
AGTACTT	19345	0.0	14.182218	12
TACTTTT	19700	0.0	13.982996	14
CTATACT	585	0.0	13.914529	4
ACTTTTT	21155	0.0	13.231152	15
CTACACT	980	0.0	12.647959	4
CGTACAC	295	1.8189894E-12	12.542374	3
CTTTTTT	22435	0.0	12.501003	16
TAGACTG	610	0.0	12.434426	5
TATGCGC	165	3.810117E-6	12.333333	10
GTATACT	320	0.0	12.140626	4
TATACTG	565	0.0	12.115045	5
TACTCCG	375	0.0	11.839999	5
TACACTG	2195	0.0	11.715262	5
>>END_MODULE