FastQCFastQC Report
Thu 9 Feb 2017
SRR2727246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4237205
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT158530.37413814059031836No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG128230.30262873757583125No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG109990.25958149298889244No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG106940.25238335176136156No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG98840.2332669766980828No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG97540.2301989165027418No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA88720.20938330810050493No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT84130.19855069556464697No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA75070.1771686760494241No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG73700.17393541261279546No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA69730.16456602878548476No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC51560.12168398743983358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48880.11535906334482282No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT48540.11455664760142595No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT45800.10809012072816868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC2950.016.9322053
ATACGGG5350.015.9065421
GAGTACT42750.015.40584811
GTACTTT42700.015.16393413
ATACGGA3800.015.0921061
TACTTTT44550.014.53423114
AGTACTT46200.014.25541212
ACTTTTT48600.013.855967515
AAGTACG9050.013.6961331
GTAGTAC2850.013.6315783
CGTATAC1102.4588034E-413.4545453
TATACTG5100.013.4215695
TAATACC3750.013.3200014
TAGACTG5450.013.2385325
TATACCG2102.046363E-913.2142855
TATACAG5100.013.0588245
TTAGACT2850.012.9824564
TTATACT4200.012.7738094
CAATACT5400.012.6759254
TTAGTAC2358.112693E-1012.5957443
Produced by FastQC (version 0.11.5)