FastQCFastQC Report
Thu 9 Feb 2017
SRR2727245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6059378
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT291810.48158408338281583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT234010.38619475464313335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129650.2139658559013813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126430.20865177911000105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC9700.018.522
CGAGACT10050.017.48756223
CACGAGA11100.015.521
TATACAC14000.014.7999993
CCCACGA12050.014.58506219
ATCTCCG12500.014.20810
GTATACG2252.7284841E-1113.97777751
GGTATCA127800.013.332161
CCACGAG13200.013.17424220
CTTATAC15400.012.9740261
TTATACA16150.012.4860682
GCCCACG14850.012.33333318
TCGTTAC14900.012.04362523
TACGGAT25000.011.98827
GTTACGG19150.011.97911225
TACACAT19500.011.8589745
TCTACAC12550.011.79282953
AACGCAG180600.011.7699345
ACGGATG25900.011.64285828
TCTCCGA16000.011.56249911
Produced by FastQC (version 0.11.5)