##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727245.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6059378
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.439582742651144	31.0	31.0	34.0	30.0	34.0
2	31.63949385563997	31.0	31.0	34.0	30.0	34.0
3	31.74477182311452	31.0	31.0	34.0	30.0	34.0
4	35.48410826985872	37.0	35.0	37.0	33.0	37.0
5	35.301166885446	37.0	35.0	37.0	33.0	37.0
6	35.307612266473555	37.0	35.0	37.0	32.0	37.0
7	35.20591271909427	37.0	35.0	37.0	32.0	37.0
8	35.17485804648596	37.0	35.0	37.0	32.0	37.0
9	36.72906394022621	39.0	35.0	39.0	32.0	39.0
10	36.54285027275077	38.0	35.0	39.0	32.0	39.0
11	36.6169134851795	38.0	35.0	39.0	32.0	39.0
12	36.47180832752141	38.0	35.0	39.0	32.0	39.0
13	36.50895174389186	38.0	35.0	39.0	32.0	39.0
14	37.50785526171168	39.0	36.0	41.0	32.0	41.0
15	37.45839969052929	39.0	36.0	41.0	32.0	41.0
16	37.36893093647566	39.0	36.0	41.0	32.0	41.0
17	37.31076539539207	39.0	36.0	41.0	32.0	41.0
18	37.22667986714148	39.0	36.0	40.0	31.0	41.0
19	37.21949629153355	39.0	36.0	40.0	31.0	41.0
20	37.09660793566601	39.0	36.0	40.0	31.0	41.0
21	36.99798609692282	39.0	36.0	40.0	31.0	41.0
22	37.30387046327197	39.0	36.0	41.0	31.0	41.0
23	37.326313690943195	39.0	36.0	41.0	31.0	41.0
24	37.317700760705144	39.0	36.0	41.0	31.0	41.0
25	37.28185797288104	39.0	36.0	41.0	31.0	41.0
26	37.12609281018613	39.0	36.0	41.0	31.0	41.0
27	37.01445808464169	39.0	36.0	41.0	31.0	41.0
28	36.876762928472196	39.0	36.0	41.0	30.0	41.0
29	36.76874573594848	39.0	36.0	41.0	30.0	41.0
30	36.649626083733345	39.0	35.0	40.0	30.0	41.0
31	36.527769021836896	39.0	35.0	40.0	30.0	41.0
32	36.3951785150225	39.0	35.0	40.0	30.0	41.0
33	36.272339009053404	38.0	35.0	40.0	30.0	41.0
34	36.15827218569299	38.0	35.0	40.0	29.0	41.0
35	36.02218792093842	38.0	35.0	40.0	29.0	41.0
36	35.88046396841392	38.0	35.0	40.0	27.0	41.0
37	35.78194114973517	38.0	34.0	40.0	27.0	41.0
38	35.63419248642352	38.0	34.0	40.0	27.0	41.0
39	35.523957079423006	38.0	34.0	40.0	26.0	41.0
40	35.38829348490885	38.0	34.0	40.0	26.0	41.0
41	35.260960118348784	38.0	34.0	40.0	26.0	41.0
42	35.1157861417459	38.0	33.0	40.0	25.0	41.0
43	34.972938311490054	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	8.0
14	17.0
15	34.0
16	83.0
17	241.0
18	490.0
19	1180.0
20	2560.0
21	4923.0
22	8978.0
23	15464.0
24	24601.0
25	36860.0
26	53051.0
27	73664.0
28	100366.0
29	131601.0
30	168891.0
31	211578.0
32	259660.0
33	316124.0
34	388562.0
35	475263.0
36	596618.0
37	777413.0
38	1106320.0
39	1304821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.554459550138645	19.784373907684913	13.428721561850077	26.232444980326363
2	19.73908873154967	22.326086934995637	32.43605201722025	25.498772316234437
3	20.396532449370216	22.770951077816896	30.319910723509906	26.51260574930298
4	16.200540715565197	16.217390629863328	32.53398286094711	35.04808579362436
5	15.14001932211524	36.76846039312946	33.0597463964123	15.031773888342995
6	35.70683327562664	34.6434072936199	14.063918771860742	15.58584065889271
7	29.55035978940413	30.94905120624592	19.91555898971809	19.585030014631865
8	28.035220776786	34.44353529355653	18.706870573184244	18.814373356473222
9	27.235155159489967	14.454206355833882	18.555831968231722	39.75480651644443
10	17.093074569700057	27.86122602022848	32.2691867053021	22.776512704769367
11	36.236376076884454	22.183448532176076	20.337929074568383	21.242246316371087
12	21.59289286788182	25.327022014470792	28.929025388414452	24.151059729232934
13	31.273787507562655	19.98969201129225	23.549232280937087	25.18728820020801
14	22.42020550624173	21.05221030937499	24.962248600433906	31.565335583949377
15	25.623686127520017	28.138944294282354	21.874307891007955	24.363061687189674
16	24.438630499698156	26.391405190433737	24.31540002950798	24.85456428036013
17	24.19556924819676	26.336085981102354	25.184829201941188	24.283515568759697
18	24.21055428461469	25.72874641588625	25.874586467455902	24.186112832043158
19	25.256800285441837	25.367059127190945	26.26820772693171	23.10793286043551
20	24.431055464768825	25.199170607940285	26.21407675837355	24.155697168917335
21	24.164179887770658	25.516711451241363	26.63745354721227	23.681655113775705
22	24.72705944405515	25.093912279445185	25.619131204555977	24.559897071943688
23	24.17822423357645	25.294724970120697	26.03128571942533	24.495765076877525
24	24.056693607825753	25.251898792252277	26.12551981407993	24.565887785842044
25	24.57179598302004	25.020290861537276	25.86151581895039	24.546397336492294
26	24.339907495455805	25.37760476405334	26.13278128547187	24.14970645501898
27	24.68614765409915	25.792267787221725	25.668674243461954	23.852910315217173
28	24.076134547143287	26.11061729438236	25.46862070661378	24.34462745186057
29	24.108992705191852	26.16664614750887	26.124958700381455	23.599402446917818
30	24.781751526311776	25.612595880303225	25.67019255111663	23.935460042268364
31	24.468996652791756	25.332616648111404	26.1524697749505	24.04591692414634
32	24.012992752721484	25.76143954049409	25.862473012906605	24.36309469387782
33	24.084204682394795	25.270052470732146	25.80426571836251	24.84147712851055
34	24.501508240614797	25.623735637552237	26.083023703092955	23.791732418740008
35	24.455975514318467	25.671067228352477	25.75528379315501	24.117673464174043
36	24.266599641085275	25.604014141385466	26.00153019006241	24.127856027466844
37	24.0555218703966	25.72062677060253	26.316859585257763	23.906991773743115
38	24.32413029852239	25.43181824933186	26.050050021635883	24.194001430509864
39	23.713737614652857	25.945616860344412	26.447434043560246	23.893211481442485
40	24.693194582018155	25.311376844289956	26.293573366771312	23.701855206920577
41	24.03132796798615	25.615038375225975	26.10061626787436	24.253017388913513
42	24.049762203315257	25.718283295744214	26.01701032680252	24.214944174138004
43	23.944124297906484	25.31992557651957	25.966081006994447	24.7698691185795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1882.0
1	1694.5
2	1507.0
3	1734.5
4	1962.0
5	1962.0
6	2934.0
7	3906.0
8	3780.5
9	3655.0
10	5613.0
11	7571.0
12	7571.0
13	18456.5
14	29342.0
15	41656.0
16	53970.0
17	44227.5
18	34485.0
19	34485.0
20	43048.0
21	51611.0
22	43478.0
23	35345.0
24	42751.0
25	50157.0
26	50157.0
27	61704.0
28	73251.0
29	84783.0
30	96315.0
31	111915.5
32	127516.0
33	127516.0
34	146703.5
35	165891.0
36	192726.0
37	219561.0
38	247561.5
39	275562.0
40	275562.0
41	305672.0
42	335782.0
43	365727.5
44	395673.0
45	427518.5
46	459364.0
47	459364.0
48	492483.5
49	525603.0
50	546483.5
51	567364.0
52	561402.0
53	555440.0
54	555440.0
55	521393.5
56	487347.0
57	451141.0
58	414935.0
59	380207.0
60	345479.0
61	345479.0
62	300762.5
63	256046.0
64	215699.5
65	175353.0
66	145262.0
67	115171.0
68	115171.0
69	94370.0
70	73569.0
71	59880.5
72	46192.0
73	37991.0
74	29790.0
75	29790.0
76	23981.5
77	18173.0
78	14620.0
79	11067.0
80	8702.0
81	6337.0
82	6337.0
83	4845.0
84	3353.0
85	2651.5
86	1950.0
87	1509.0
88	1068.0
89	1068.0
90	769.0
91	470.0
92	341.0
93	212.0
94	163.0
95	114.0
96	114.0
97	68.0
98	22.0
99	18.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6059378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.15861070479377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76686501120533	35.73060091386092
2	11.671544727099983	11.00827668217793
3	4.314228335080121	6.1035904363690205
4	2.192696028955892	4.136179936939126
5	1.2975565255365005	3.059548152762032
6	0.8563567986152383	2.42307581341797
7	0.6010889589402937	1.9842564149530555
8	0.45787082144396857	1.7274041457248206
9	0.3677202731891167	1.5607059490427373
>10	2.251116930153736	19.819049838266253
>50	0.1616820025397629	5.166912301791673
>100	0.058290342751447705	4.635770996721203
>500	0.0020005359126605755	0.6503493536780537
>1k	8.42321636700582E-4	0.7001460096101573
>5k	0.0	0.0
>10k+	1.40386939397248E-4	1.294133054685057
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29181	0.48158408338281583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23401	0.38619475464313335	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12965	0.2139658559013813	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12643	0.20865177911000105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.650334407260943E-5	8.251672036304716E-5
2	0.0	0.0	0.0	1.650334407260943E-5	8.251672036304716E-5
3	1.650334407260943E-5	0.0	0.0	1.650334407260943E-5	8.251672036304716E-5
4	3.300668814521886E-5	0.0	0.0	8.251672036304716E-5	1.6503344072609432E-4
5	3.300668814521886E-5	0.0	0.0	1.3202675258087545E-4	1.6503344072609432E-4
6	3.300668814521886E-5	0.0	0.0	1.3202675258087545E-4	2.475501610891415E-4
7	3.300668814521886E-5	0.0	0.0	1.485300966534849E-4	2.640535051617509E-4
8	3.300668814521886E-5	0.0	0.0	1.8153678479870377E-4	3.3006688145218864E-4
9	4.95100322178283E-5	0.0	0.0	1.980401288713132E-4	4.1258360181523583E-4
10	8.251672036304716E-5	0.0	0.0	2.475501610891415E-4	4.1258360181523583E-4
11	8.251672036304716E-5	0.0	0.0	2.475501610891415E-4	4.290869458878452E-4
12	8.251672036304716E-5	0.0	0.0	2.475501610891415E-4	4.290869458878452E-4
13	8.251672036304716E-5	0.0	0.0	2.475501610891415E-4	4.290869458878452E-4
14	8.251672036304716E-5	0.0	0.0	2.640535051617509E-4	6.436304188317678E-4
15	8.251672036304716E-5	1.650334407260943E-5	0.0	2.640535051617509E-4	6.766371069769867E-4
16	8.251672036304716E-5	1.650334407260943E-5	0.0	2.640535051617509E-4	8.086638595578622E-4
17	8.251672036304716E-5	1.650334407260943E-5	0.0	2.640535051617509E-4	8.086638595578622E-4
18	8.251672036304716E-5	1.650334407260943E-5	0.0	2.640535051617509E-4	9.571939562113471E-4
19	9.90200644356566E-5	3.300668814521886E-5	0.0	2.640535051617509E-4	9.571939562113471E-4
20	1.1552340850826603E-4	3.300668814521886E-5	0.0	2.640535051617509E-4	9.571939562113471E-4
21	1.1552340850826603E-4	3.300668814521886E-5	0.0	2.970601933069698E-4	9.90200644356566E-4
22	1.1552340850826603E-4	3.300668814521886E-5	0.0	4.1258360181523583E-4	0.001023207332501785
23	1.1552340850826603E-4	3.300668814521886E-5	0.0	4.620936340330641E-4	0.0014357909343170206
24	1.1552340850826603E-4	3.300668814521886E-5	0.0	6.766371069769867E-4	0.0014687976224622395
25	1.1552340850826603E-4	3.300668814521886E-5	0.0	7.591538273400339E-4	0.001485300966534849
26	1.1552340850826603E-4	3.300668814521886E-5	0.0	0.0011717374291552697	0.0015183076546800677
27	1.3202675258087545E-4	3.300668814521886E-5	0.0	0.003284165470449277	0.0015348109987526773
28	1.3202675258087545E-4	3.300668814521886E-5	0.0	0.008086638595578621	0.0015348109987526773
29	1.3202675258087545E-4	3.300668814521886E-5	0.0	0.016998444394787714	0.0015513143428252866
30	1.3202675258087545E-4	3.300668814521886E-5	0.0	0.027247021063878173	0.0015513143428252866
31	1.3202675258087545E-4	3.300668814521886E-5	0.0	0.05355335151561761	0.0015513143428252866
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAGAC	970	0.0	18.5	22
CGAGACT	1005	0.0	17.487562	23
CACGAGA	1110	0.0	15.5	21
TATACAC	1400	0.0	14.799999	3
CCCACGA	1205	0.0	14.585062	19
ATCTCCG	1250	0.0	14.208	10
GTATACG	225	2.7284841E-11	13.9777775	1
GGTATCA	12780	0.0	13.33216	1
CCACGAG	1320	0.0	13.174242	20
CTTATAC	1540	0.0	12.974026	1
TTATACA	1615	0.0	12.486068	2
GCCCACG	1485	0.0	12.333333	18
TCGTTAC	1490	0.0	12.043625	23
TACGGAT	2500	0.0	11.988	27
GTTACGG	1915	0.0	11.979112	25
TACACAT	1950	0.0	11.858974	5
TCTACAC	1255	0.0	11.7928295	3
AACGCAG	18060	0.0	11.769934	5
ACGGATG	2590	0.0	11.642858	28
TCTCCGA	1600	0.0	11.562499	11
>>END_MODULE