FastQCFastQC Report
Thu 9 Feb 2017
SRR2727244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6279624
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401250.6389713779041547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT234780.3738758881104983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166230.26471330130593806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121090.19283001657424073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC4650.013.1290324
TAATCCC13800.011.931165
GTATTAG5800.011.8017241
TCGTTAC11650.011.75107323
TATACTG8750.011.62857155
GTACTAG3750.011.3466661
TAAGACT8700.011.057474
TTAATCC17000.010.9911774
AACGCAG268450.010.8126285
TAGTACT5150.010.7766994
GAGTACT148000.010.737511
TAGACTG7300.010.6438365
TTTAATC19750.010.584813
CCTATAC6650.010.5714283
AGTACTT151150.010.55044712
GTACTTT151650.010.49126313
CTCGTTA12550.010.46613522
CTAGTAC4450.010.3932593
TAATACT7150.010.3496494
CCCTATA4700.010.2340422
Produced by FastQC (version 0.11.5)