##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727244.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6279624
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.439639379682607	31.0	31.0	34.0	30.0	34.0
2	31.633369450145423	31.0	31.0	34.0	30.0	34.0
3	31.740689251458367	31.0	31.0	34.0	30.0	34.0
4	35.48139617913429	37.0	35.0	37.0	33.0	37.0
5	35.297997618965724	37.0	35.0	37.0	33.0	37.0
6	35.31070315674951	37.0	35.0	37.0	32.0	37.0
7	35.20142750585067	37.0	35.0	37.0	32.0	37.0
8	35.162732195430806	37.0	35.0	37.0	32.0	37.0
9	36.71658876391325	39.0	35.0	39.0	32.0	39.0
10	36.538415835088216	38.0	35.0	39.0	32.0	39.0
11	36.61108435791697	38.0	35.0	39.0	32.0	39.0
12	36.463858823394524	38.0	35.0	39.0	32.0	39.0
13	36.49056758812311	38.0	35.0	39.0	32.0	39.0
14	37.47432489588549	39.0	36.0	41.0	32.0	41.0
15	37.42967540731738	39.0	36.0	41.0	32.0	41.0
16	37.339054058013666	39.0	36.0	41.0	32.0	41.0
17	37.28760368455181	39.0	36.0	41.0	32.0	41.0
18	37.21452207966592	39.0	36.0	40.0	31.0	41.0
19	37.20702417851769	39.0	36.0	40.0	31.0	41.0
20	37.094401511937654	39.0	36.0	40.0	31.0	41.0
21	36.99462133401617	39.0	36.0	40.0	31.0	41.0
22	37.302979127412726	39.0	36.0	41.0	31.0	41.0
23	37.32343990659314	39.0	36.0	41.0	31.0	41.0
24	37.31338834936614	39.0	36.0	41.0	31.0	41.0
25	37.27640779129451	39.0	36.0	41.0	31.0	41.0
26	37.11531773239926	39.0	36.0	41.0	31.0	41.0
27	36.998215657497965	39.0	36.0	41.0	30.0	41.0
28	36.859455279488074	39.0	36.0	41.0	30.0	41.0
29	36.748215179762354	39.0	36.0	40.0	30.0	41.0
30	36.61899231546347	39.0	35.0	40.0	30.0	41.0
31	36.4991679438132	39.0	35.0	40.0	30.0	41.0
32	36.370995142384324	38.0	35.0	40.0	30.0	41.0
33	36.25042805110625	38.0	35.0	40.0	30.0	41.0
34	36.136041903145795	38.0	35.0	40.0	29.0	41.0
35	35.99373959332597	38.0	35.0	40.0	28.0	41.0
36	35.850899353209684	38.0	35.0	40.0	27.0	41.0
37	35.741106633136	38.0	34.0	40.0	27.0	41.0
38	35.59101723287891	38.0	34.0	40.0	27.0	41.0
39	35.4740584786605	38.0	34.0	40.0	26.0	41.0
40	35.32777153536581	38.0	34.0	40.0	26.0	41.0
41	35.189550839349614	38.0	33.0	40.0	25.0	41.0
42	35.02656528480049	38.0	33.0	40.0	25.0	41.0
43	34.875634114399205	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	6.0
13	8.0
14	21.0
15	27.0
16	110.0
17	227.0
18	550.0
19	1201.0
20	2616.0
21	5178.0
22	9452.0
23	15983.0
24	25915.0
25	38193.0
26	55483.0
27	77813.0
28	104830.0
29	137593.0
30	176669.0
31	220849.0
32	271232.0
33	329861.0
34	404392.0
35	495038.0
36	619470.0
37	813247.0
38	1141741.0
39	1331917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52643534071467	19.124250114338057	13.265077654330895	26.08423689061638
2	19.76678858479425	22.51443717012356	31.815296584636275	25.903477660445912
3	20.885852401353965	22.512446605083362	29.821259999006312	26.780440994556358
4	16.289669572573136	15.768364475325274	31.762761592095323	36.17920436000627
5	15.544465719603595	36.7687619513525	32.39394587956221	15.292826449481689
6	36.62803696527053	33.83872983477992	13.47327483301548	16.059958366934072
7	30.25498023448538	30.392870655950098	19.387785001140195	19.964364108424327
8	28.961351826160293	33.97053708948179	18.153857619500783	18.91425346485713
9	27.344153089420644	14.356369107449746	18.06633645581328	40.233141347316334
10	17.48418376641659	27.898740434140645	31.622578039704287	22.99449775973848
11	36.6438659384702	21.982446719739908	19.496915738904114	21.87677160288578
12	22.074490447198748	25.211286535626975	28.445731782667245	24.268491234507035
13	32.08626822242861	19.40476053980302	23.119489319742712	25.389481918025663
14	22.818754753469317	20.71337392175073	24.825244313990773	31.64262701078918
15	26.180580238562058	27.701085287908956	21.63549919549323	24.482835278035754
16	24.815976243163604	25.735665065296903	24.041534970883607	25.406823720655886
17	24.68434415818527	25.950853108402665	25.069590153805386	24.295212579606677
18	24.701080829043267	25.03110058818808	25.649354165153838	24.618464417614813
19	25.574715938406506	25.151633282502267	25.965838082025293	23.307812697065938
20	24.825562804397205	25.00644943073025	25.83154660215325	24.336441162719296
21	24.338352105157888	25.452335999735016	26.07058957670077	24.138722318406323
22	24.934709466681447	24.993789437074575	25.1734817243835	24.89801937186048
23	24.49442832882988	25.044795675664655	25.45087094386543	25.009905051640036
24	24.37521736970239	25.104862966317725	25.63994277364377	24.87997689033611
25	24.961351189179478	24.712928672162537	25.375006529053334	24.95071360960465
26	24.640089916211544	25.231239959589935	25.73713012116649	24.391540003032027
27	24.986703025531465	25.55047881847703	25.451635320840865	24.011182835150642
28	24.434711377623884	26.07726195071552	25.208818234977127	24.27920843668347
29	24.442689562305002	26.054187320769522	25.68502509067422	23.818098026251253
30	24.772136038718244	25.68655384462509	25.447272002272747	24.094038114383917
31	24.580659606371334	25.306706261393998	25.778900137970044	24.333733994264627
32	24.111953199745717	25.754455999276388	25.449039624028448	24.684551176949448
33	24.170332491244697	25.45515463983194	25.400883874575932	24.973628994347433
34	24.665298431880636	25.695614896688078	25.43923011951034	24.19985655192094
35	24.489189161644074	25.870720922144386	25.332137720347585	24.307952195863955
36	24.457276422919588	25.85933489011444	25.43515344230801	24.24823524465796
37	24.12588715502712	25.91803267201985	25.691984106054754	24.264096066898272
38	24.441479298760562	25.791162018617676	25.536688183878525	24.230670498743237
39	23.82383085356703	26.243131754385296	25.80912487754044	24.123912514507236
40	24.768839663011672	25.736954951442954	25.74550641885565	23.748698966689723
41	24.3400560288323	25.866628320421732	25.533726223098707	24.25958942764726
42	24.143181184096374	25.95894276472604	25.589653138468165	24.308222912709425
43	24.37214393728032	25.557820022345286	25.394561840008258	24.67547420036614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	583.0
1	822.0
2	1061.0
3	1413.0
4	1765.0
5	1765.0
6	2929.0
7	4093.0
8	4009.0
9	3925.0
10	5784.5
11	7644.0
12	7644.0
13	20663.5
14	33683.0
15	51206.0
16	68729.0
17	51917.0
18	35105.0
19	35105.0
20	43815.0
21	52525.0
22	41717.5
23	30910.0
24	37448.0
25	43986.0
26	43986.0
27	55098.5
28	66211.0
29	77962.5
30	89714.0
31	103907.5
32	118101.0
33	118101.0
34	134782.0
35	151463.0
36	177219.5
37	202976.0
38	233268.5
39	263561.0
40	263561.0
41	296035.0
42	328509.0
43	361616.0
44	394723.0
45	430840.5
46	466958.0
47	466958.0
48	504316.5
49	541675.0
50	563678.0
51	585681.0
52	587990.5
53	590300.0
54	590300.0
55	561655.0
56	533010.0
57	488437.5
58	443865.0
59	408530.0
60	373195.0
61	373195.0
62	328428.0
63	283661.0
64	241208.5
65	198756.0
66	165683.0
67	132610.0
68	132610.0
69	109515.5
70	86421.0
71	70890.0
72	55359.0
73	45682.5
74	36006.0
75	36006.0
76	29084.5
77	22163.0
78	18027.5
79	13892.0
80	10912.0
81	7932.0
82	7932.0
83	6126.0
84	4320.0
85	3323.0
86	2326.0
87	1788.5
88	1251.0
89	1251.0
90	914.5
91	578.0
92	409.5
93	241.0
94	175.0
95	109.0
96	109.0
97	71.0
98	33.0
99	24.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6279624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.5724604457219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06601053222589	36.947132878404084
2	11.821325582187184	11.48381738513575
3	4.251777958681575	6.195579501661591
4	2.1847672876755677	4.244780906549146
5	1.2814557447231043	3.112172923675305
6	0.8191612017509324	2.3873205042430317
7	0.5654741632164764	1.9226529998136996
8	0.4555570811906295	1.77020226455203
9	0.3355962885251289	1.4670663705106108
>10	2.0384876468478126	18.333666248655003
>50	0.1295593676356853	4.256258108615947
>100	0.04650574007766393	3.9398539399767416
>500	0.0024553660492120893	0.8072653055463879
>1k	0.0017023515630622088	1.576467158669644
>5k	3.273753002636743E-5	0.08750553529776899
>10k+	1.3095012010546972E-4	1.4682579686933488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40125	0.6389713779041547	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23478	0.3738758881104983	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16623	0.26471330130593806	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12109	0.19283001657424073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.777356096479662E-5
2	1.5924520321598872E-5	0.0	0.0	0.0	4.777356096479662E-5
3	1.5924520321598872E-5	0.0	0.0	0.0	4.777356096479662E-5
4	1.5924520321598872E-5	0.0	0.0	1.5924520321598872E-5	4.777356096479662E-5
5	3.1849040643197744E-5	0.0	0.0	1.114716422511921E-4	4.777356096479662E-5
6	3.1849040643197744E-5	0.0	0.0	1.114716422511921E-4	7.962260160799437E-5
7	3.1849040643197744E-5	0.0	0.0	1.4332068289438986E-4	9.554712192959324E-5
8	4.777356096479662E-5	1.5924520321598872E-5	0.0	1.4332068289438986E-4	1.114716422511921E-4
9	4.777356096479662E-5	1.5924520321598872E-5	0.0	1.751697235375876E-4	1.751697235375876E-4
10	4.777356096479662E-5	1.5924520321598872E-5	0.0	1.9109424385918648E-4	1.751697235375876E-4
11	4.777356096479662E-5	1.5924520321598872E-5	0.0	1.9109424385918648E-4	1.751697235375876E-4
12	4.777356096479662E-5	1.5924520321598872E-5	0.0	1.9109424385918648E-4	1.751697235375876E-4
13	6.369808128639549E-5	1.5924520321598872E-5	0.0	1.9109424385918648E-4	1.9109424385918648E-4
14	6.369808128639549E-5	1.5924520321598872E-5	0.0	2.388678048239831E-4	3.8218848771837295E-4
15	6.369808128639549E-5	1.5924520321598872E-5	0.0	2.5479232514558195E-4	4.2996204868316957E-4
16	6.369808128639549E-5	1.5924520321598872E-5	0.0	2.7071684546718086E-4	4.936601299695651E-4
17	6.369808128639549E-5	1.5924520321598872E-5	0.0	2.7071684546718086E-4	5.095846502911639E-4
18	6.369808128639549E-5	1.5924520321598872E-5	0.0	2.7071684546718086E-4	5.573582112559605E-4
19	9.554712192959324E-5	1.5924520321598872E-5	0.0	3.0256588611037857E-4	5.732827315775594E-4
20	9.554712192959324E-5	1.5924520321598872E-5	0.0	3.3441492675357633E-4	5.892072518991583E-4
21	9.554712192959324E-5	1.5924520321598872E-5	0.0	5.255091706127628E-4	6.051317722207571E-4
22	9.554712192959324E-5	1.5924520321598872E-5	0.0	8.439995770447402E-4	6.529053331855538E-4
23	9.554712192959324E-5	1.5924520321598872E-5	1.5924520321598872E-5	0.001003244780260729	8.439995770447402E-4
24	9.554712192959324E-5	1.5924520321598872E-5	1.5924520321598872E-5	0.001385433267979102	8.599240973663391E-4
25	9.554712192959324E-5	1.5924520321598872E-5	1.5924520321598872E-5	0.001751697235375876	8.75848617687938E-4
26	9.554712192959324E-5	1.5924520321598872E-5	1.5924520321598872E-5	0.002245357365345441	9.236221786527347E-4
27	1.114716422511921E-4	1.5924520321598872E-5	1.5924520321598872E-5	0.003487469950430153	9.236221786527347E-4
28	1.2739616257279097E-4	1.5924520321598872E-5	1.5924520321598872E-5	0.006799770177322718	9.554712192959324E-4
29	1.2739616257279097E-4	1.5924520321598872E-5	1.5924520321598872E-5	0.014156898565901398	9.554712192959324E-4
30	1.2739616257279097E-4	1.5924520321598872E-5	1.5924520321598872E-5	0.02469893101879985	9.554712192959324E-4
31	1.5924520321598874E-4	1.5924520321598872E-5	1.5924520321598872E-5	0.048681258623127756	9.713957396175313E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACC	465	0.0	13.129032	4
TAATCCC	1380	0.0	11.93116	5
GTATTAG	580	0.0	11.801724	1
TCGTTAC	1165	0.0	11.751073	23
TATACTG	875	0.0	11.6285715	5
GTACTAG	375	0.0	11.346666	1
TAAGACT	870	0.0	11.05747	4
TTAATCC	1700	0.0	10.991177	4
AACGCAG	26845	0.0	10.812628	5
TAGTACT	515	0.0	10.776699	4
GAGTACT	14800	0.0	10.7375	11
TAGACTG	730	0.0	10.643836	5
TTTAATC	1975	0.0	10.58481	3
CCTATAC	665	0.0	10.571428	3
AGTACTT	15115	0.0	10.550447	12
GTACTTT	15165	0.0	10.491263	13
CTCGTTA	1255	0.0	10.466135	22
CTAGTAC	445	0.0	10.393259	3
TAATACT	715	0.0	10.349649	4
CCCTATA	470	0.0	10.234042	2
>>END_MODULE