FastQCFastQC Report
Thu 9 Feb 2017
SRR2727243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5020595
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123560.24610628819890867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105740.21061248716536585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53220.10600337211027777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52620.10480829463440092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG609.242725E-418.52
GGTATCA77050.012.8215451
TCTATAC4800.012.7187493
GTATTAG5000.011.4699991
TATACTG8450.011.1656815
TATACCG2002.6620091E-611.15
GTATAGA5900.010.9745761
CGTACTA2207.021208E-710.93181829
ATAGCAC7150.010.8671333
TAGACAG11100.010.8333335
TATGACT6550.010.7328244
GGGTAAG41500.010.6987951
CTAGGAC7650.010.6405233
TAGACTG7700.010.5714285
ACCGTAT2104.7752565E-610.5714288
GGACCGT3003.3178367E-910.4833336
TAGGACT7250.010.4620694
CGTATAC1604.8270077E-410.406253
TCGTTAC10800.010.27777723
TAGGACA6850.010.26277354
Produced by FastQC (version 0.11.5)