##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727243.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5020595
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177882900333525	31.0	31.0	33.0	30.0	34.0
2	31.396236501848886	31.0	31.0	34.0	30.0	34.0
3	31.4862172710605	31.0	31.0	34.0	30.0	34.0
4	35.26374304240832	37.0	35.0	37.0	33.0	37.0
5	35.053659974564766	35.0	35.0	37.0	32.0	37.0
6	35.09202355497705	36.0	35.0	37.0	32.0	37.0
7	35.00311875385288	36.0	35.0	37.0	32.0	37.0
8	34.96661471399306	36.0	35.0	37.0	32.0	37.0
9	36.503954610957464	38.0	35.0	39.0	32.0	39.0
10	36.26922625704722	38.0	35.0	39.0	32.0	39.0
11	36.36435044053543	38.0	35.0	39.0	32.0	39.0
12	36.19313826349267	38.0	35.0	39.0	32.0	39.0
13	36.24148731375465	38.0	35.0	39.0	32.0	39.0
14	37.19374337105462	39.0	36.0	40.0	31.0	41.0
15	37.12856364634072	39.0	36.0	40.0	31.0	41.0
16	37.0261269829572	39.0	36.0	40.0	31.0	41.0
17	36.9465415951695	38.0	36.0	40.0	31.0	41.0
18	36.84519543998271	38.0	36.0	40.0	31.0	41.0
19	36.82528385579797	39.0	36.0	40.0	31.0	41.0
20	36.692741597360474	38.0	35.0	40.0	30.0	41.0
21	36.573390803281285	38.0	35.0	40.0	30.0	41.0
22	36.90010407133019	39.0	36.0	40.0	31.0	41.0
23	36.9183965247147	39.0	36.0	40.0	31.0	41.0
24	36.900398657927994	39.0	36.0	40.0	30.0	41.0
25	36.86272802327214	39.0	36.0	40.0	30.0	41.0
26	36.6875922076965	39.0	36.0	40.0	30.0	41.0
27	36.567927904959475	39.0	35.0	40.0	30.0	41.0
28	36.4165787122841	39.0	35.0	40.0	30.0	41.0
29	36.305718744491436	38.0	35.0	40.0	30.0	41.0
30	36.18476734331289	38.0	35.0	40.0	29.0	41.0
31	36.065168769837044	38.0	35.0	40.0	29.0	41.0
32	35.93341307155825	38.0	35.0	40.0	28.0	41.0
33	35.80239871967366	38.0	34.0	40.0	27.0	41.0
34	35.68461447298577	38.0	34.0	40.0	27.0	41.0
35	35.54695409607825	38.0	34.0	40.0	27.0	41.0
36	35.39980599908975	38.0	34.0	40.0	26.0	41.0
37	35.29909343414476	38.0	34.0	40.0	26.0	41.0
38	35.15366764297857	38.0	33.0	40.0	25.0	41.0
39	35.03684324268339	38.0	33.0	40.0	25.0	41.0
40	34.90689290811149	38.0	33.0	40.0	25.0	41.0
41	34.78211925080593	38.0	33.0	40.0	24.0	41.0
42	34.65172414823343	38.0	33.0	40.0	24.0	41.0
43	34.5217827767426	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	5.0
14	19.0
15	35.0
16	107.0
17	264.0
18	598.0
19	1292.0
20	2873.0
21	5510.0
22	9845.0
23	16356.0
24	25277.0
25	37570.0
26	53607.0
27	72730.0
28	96919.0
29	125372.0
30	158537.0
31	194710.0
32	235602.0
33	284282.0
34	344339.0
35	418876.0
36	519336.0
37	669683.0
38	960129.0
39	786717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.24130506443957	21.09737989222393	13.969579302851553	25.691735740484944
2	21.061647075695213	22.975244965985105	31.236835474679793	24.72627248363989
3	21.38437774805576	23.50822960226826	29.528452304955888	25.578940344720095
4	17.60934709929799	16.81280804366813	32.01461181393839	33.563233043095494
5	15.41673845430671	36.89763862649746	32.850568508314254	14.835054410881579
6	36.0560252320691	35.05937045310367	13.792528574800397	15.09207574002683
7	29.857397380190992	30.944400016332725	19.75909229882116	19.439110304655124
8	27.983954093090556	34.65093280776482	18.591561358763254	18.773551740381368
9	27.341898719175717	14.29786708547493	18.242897505176177	40.117336690173175
10	16.971275316969404	27.862215534214567	32.53761755329796	22.62889159551806
11	36.92584245492815	21.906287999729116	20.00027885141104	21.167590693931697
12	21.606861338148168	25.095690849391357	28.9653915521965	24.332056260263972
13	31.314276495116616	20.08068764757962	23.229597288767568	25.3754385685362
14	22.635125916350553	20.770287187076434	24.479469066913385	32.11511782965963
15	25.881275028159013	28.095116216305037	21.43136421081565	24.592244544720295
16	24.91597111497741	26.555497904132874	23.80116300956361	24.72736797132611
17	24.511815830593783	26.30016960141178	24.747026995804283	24.44098757219015
18	24.47490785454712	25.87651463621344	25.424755432374052	24.223822076865392
19	25.51723451104899	25.474331229665008	25.588062769452623	23.42037148983338
20	24.964351834792488	25.08808218946161	25.486720199498265	24.460845776247638
21	24.71507859128251	25.429794675730665	25.77879713460257	24.076329598384255
22	25.096308306087227	25.0043470943185	24.983751925817558	24.91559267377671
23	24.690300651615992	25.292520109668278	25.271148140808013	24.746031097907718
24	24.53255042480025	25.20800821416585	25.326081868782484	24.933359492251416
25	25.200499144025756	24.919596183320902	25.051214049330806	24.828690623322537
26	24.86699285642439	25.26909659114109	25.243999964147672	24.619910588286846
27	25.179963729398608	25.676757436120617	24.965148553109742	24.178130281371033
28	24.641919931800913	25.983752921715453	24.815086658055467	24.559240488428163
29	24.765909219923138	25.95373655911301	25.327794813164573	23.952559407799274
30	25.505283736290217	25.4016705191317	24.811680687249222	24.281365057328863
31	25.085990803878822	25.229081413657145	25.38880351830809	24.296124264155942
32	24.66193747952185	25.53159535871744	25.159547822519045	24.646919339241663
33	24.67500365992477	25.09403765888306	25.13082612718214	25.100132554010035
34	25.05113437749908	25.266308077030708	25.420194220007787	24.26236332546242
35	25.024145544502197	25.387787702453597	25.079856072835987	24.508210680208222
36	25.01131837959445	25.49187895060247	25.12001067602545	24.37679199377763
37	24.582942858366387	25.61178505734878	25.664607481782536	24.140664602502294
38	25.136383237444964	25.232347958757877	25.211912133920382	24.419356669876777
39	24.379540671972148	25.73350369826684	25.665384282141858	24.221571347619157
40	25.384700418974244	25.07535461434352	25.50402890494055	24.035916061741684
41	24.752803203604355	25.354445040876627	25.31419084789751	24.578560907621508
42	24.666578363719836	25.456544493232375	25.36253173179673	24.514345411251057
43	24.71406277542801	25.07384084954074	25.258301057942333	24.95379531708891
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	491.0
1	511.0
2	531.0
3	595.5
4	660.0
5	660.0
6	1082.0
7	1504.0
8	1533.5
9	1563.0
10	2333.5
11	3104.0
12	3104.0
13	7908.0
14	12712.0
15	18199.0
16	23686.0
17	20091.5
18	16497.0
19	16497.0
20	22026.0
21	27555.0
22	26192.5
23	24830.0
24	31068.5
25	37307.0
26	37307.0
27	46199.5
28	55092.0
29	65450.5
30	75809.0
31	89528.5
32	103248.0
33	103248.0
34	120615.5
35	137983.0
36	161448.0
37	184913.0
38	208994.0
39	233075.0
40	233075.0
41	257698.0
42	282321.0
43	308620.5
44	334920.0
45	362951.0
46	390982.0
47	390982.0
48	419325.5
49	447669.0
50	461947.0
51	476225.0
52	472309.5
53	468394.0
54	468394.0
55	444180.5
56	419967.0
57	385649.5
58	351332.0
59	320338.0
60	289344.0
61	289344.0
62	252054.0
63	214764.0
64	181239.5
65	147715.0
66	121926.5
67	96138.0
68	96138.0
69	78837.0
70	61536.0
71	49980.5
72	38425.0
73	31468.0
74	24511.0
75	24511.0
76	19832.0
77	15153.0
78	12157.0
79	9161.0
80	7216.5
81	5272.0
82	5272.0
83	4071.0
84	2870.0
85	2264.5
86	1659.0
87	1303.5
88	948.0
89	948.0
90	702.5
91	457.0
92	307.5
93	158.0
94	121.0
95	84.0
96	84.0
97	52.0
98	20.0
99	15.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5020595.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.19014501976571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37793517905021	43.015018187222935
2	10.215301323440661	11.07137320275708
3	3.641295730304614	5.919670310551822
4	1.8609381874580277	4.033780410046819
5	1.1037559957194198	2.990634873723563
6	0.7324310803397213	2.38143278763559
7	0.49422051896262104	1.8747317116029814
8	0.3790838939521125	1.643408895033797
9	0.3007311484318097	1.4666998090932406
>10	1.758908486993865	17.440741238363195
>50	0.10138581502516908	3.710812058950418
>100	0.03213442052819689	2.9479138774344738
>500	0.001178492120758503	0.42500574897748444
>1k	5.524165837936728E-4	0.4099372133740672
>5k	7.365554450536887E-5	0.21122513345649407
>10k+	7.365554450536887E-5	0.4576145417760213
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12356	0.24610628819890867	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10574	0.21061248716536585	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5322	0.10600337211027777	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5262	0.10480829463440092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9917957931281054E-5	7.967183172512421E-5
2	0.0	0.0	0.0	1.9917957931281054E-5	7.967183172512421E-5
3	0.0	0.0	0.0	1.9917957931281054E-5	7.967183172512421E-5
4	0.0	0.0	0.0	7.967183172512421E-5	7.967183172512421E-5
5	0.0	0.0	0.0	9.958978965640527E-5	7.967183172512421E-5
6	0.0	0.0	0.0	1.1950774758768632E-4	1.1950774758768632E-4
7	0.0	0.0	0.0	1.1950774758768632E-4	1.3942570551896737E-4
8	0.0	0.0	0.0	1.1950774758768632E-4	1.5934366345024843E-4
9	0.0	0.0	0.0	1.3942570551896737E-4	2.7885141103793474E-4
10	0.0	0.0	0.0	1.3942570551896737E-4	2.7885141103793474E-4
11	0.0	0.0	0.0	1.5934366345024843E-4	2.7885141103793474E-4
12	0.0	0.0	0.0	1.5934366345024843E-4	2.7885141103793474E-4
13	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	2.7885141103793474E-4
14	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	4.3819507448818315E-4
15	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	4.3819507448818315E-4
16	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	5.377848641445885E-4
17	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	5.377848641445885E-4
18	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	5.776207800071506E-4
19	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	5.776207800071506E-4
20	0.0	1.9917957931281054E-5	0.0	1.5934366345024843E-4	5.975387379384316E-4
21	0.0	1.9917957931281054E-5	0.0	1.9917957931281054E-4	5.975387379384316E-4
22	0.0	1.9917957931281054E-5	0.0	1.9917957931281054E-4	5.975387379384316E-4
23	0.0	1.9917957931281054E-5	0.0	1.9917957931281054E-4	7.768003593199611E-4
24	0.0	1.9917957931281054E-5	0.0	2.1909753724409157E-4	7.768003593199611E-4
25	0.0	3.983591586256211E-5	0.0	3.1868732690049686E-4	7.768003593199611E-4
26	0.0	3.983591586256211E-5	0.0	3.983591586256211E-4	7.768003593199611E-4
27	0.0	3.983591586256211E-5	0.0	0.001075569728289177	7.768003593199611E-4
28	0.0	3.983591586256211E-5	0.0	0.0024897447414101315	7.768003593199611E-4
29	1.9917957931281054E-5	3.983591586256211E-5	0.0	0.006015223295246878	7.768003593199611E-4
30	1.9917957931281054E-5	3.983591586256211E-5	0.0	0.010118322629090775	7.768003593199611E-4
31	1.9917957931281054E-5	3.983591586256211E-5	0.0	0.022268276967172217	7.967183172512422E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	60	9.242725E-4	18.5	2
GGTATCA	7705	0.0	12.821545	1
TCTATAC	480	0.0	12.718749	3
GTATTAG	500	0.0	11.469999	1
TATACTG	845	0.0	11.165681	5
TATACCG	200	2.6620091E-6	11.1	5
GTATAGA	590	0.0	10.974576	1
CGTACTA	220	7.021208E-7	10.931818	29
ATAGCAC	715	0.0	10.867133	3
TAGACAG	1110	0.0	10.833333	5
TATGACT	655	0.0	10.732824	4
GGGTAAG	4150	0.0	10.698795	1
CTAGGAC	765	0.0	10.640523	3
TAGACTG	770	0.0	10.571428	5
ACCGTAT	210	4.7752565E-6	10.571428	8
GGACCGT	300	3.3178367E-9	10.483333	6
TAGGACT	725	0.0	10.462069	4
CGTATAC	160	4.8270077E-4	10.40625	3
TCGTTAC	1080	0.0	10.277777	23
TAGGACA	685	0.0	10.2627735	4
>>END_MODULE