FastQCFastQC Report
Thu 9 Feb 2017
SRR2727242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5622911
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT445340.7920096903543379No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT296890.5280005321087244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT275770.4904399162640134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155400.27636930408466365No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT79090.14065668121014185No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA73160.1301105423863191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG19300.017.8290145
CTTATAC34200.017.41812937
CAAGACG20250.016.8098774
TATACCG2055.456968E-1215.3414645
TCTAGCG12650.015.20948528
CTAGCGG13000.014.829
GACGGAC22650.014.3752767
GCGTTAT15600.014.34935951
ACGGACC22650.014.2935988
TCTTATA55850.014.21038437
CGGTCCA15250.014.07213110
CGGACCA23350.013.9443269
GGGTATA2800.013.8751
AGACGGA24250.013.7319586
CGCAAGA23900.013.7008372
TAGCGGC14350.013.66550430
CGCCGGT15000.013.5666667
CGCAATA13950.013.52688136
ATACCGT15700.013.4331216
CGTTATT15950.013.3385572
Produced by FastQC (version 0.11.5)