##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727242.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5622911
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240910268720242	31.0	31.0	34.0	30.0	34.0
2	31.463391826760194	31.0	31.0	34.0	30.0	34.0
3	31.613832408160114	31.0	31.0	34.0	30.0	34.0
4	35.42395976034477	37.0	35.0	37.0	33.0	37.0
5	35.24362701099128	37.0	35.0	37.0	33.0	37.0
6	35.25072671433	37.0	35.0	37.0	32.0	37.0
7	35.148773651227984	37.0	35.0	37.0	32.0	37.0
8	35.158746777247586	37.0	35.0	37.0	32.0	37.0
9	36.73110049225392	39.0	35.0	39.0	32.0	39.0
10	36.467391000853475	38.0	35.0	39.0	32.0	39.0
11	36.62586994530057	39.0	35.0	39.0	32.0	39.0
12	36.48048119559424	38.0	35.0	39.0	32.0	39.0
13	36.546628072185385	38.0	35.0	39.0	32.0	39.0
14	37.56164218142524	39.0	36.0	41.0	32.0	41.0
15	37.55698089477141	39.0	36.0	41.0	32.0	41.0
16	37.507758881476164	39.0	36.0	41.0	32.0	41.0
17	37.4913438964266	39.0	36.0	41.0	32.0	41.0
18	37.431636033364214	39.0	36.0	41.0	32.0	41.0
19	37.440295249204546	39.0	36.0	41.0	32.0	41.0
20	37.36804850725896	39.0	36.0	41.0	31.0	41.0
21	37.266938068199906	39.0	36.0	41.0	31.0	41.0
22	37.18905705603379	39.0	36.0	40.0	31.0	41.0
23	37.0737664174304	39.0	36.0	40.0	31.0	41.0
24	36.93278534908342	39.0	36.0	40.0	31.0	41.0
25	36.812901182323536	39.0	35.0	40.0	30.0	41.0
26	36.5855952548422	39.0	35.0	40.0	30.0	41.0
27	36.41213385735609	38.0	35.0	40.0	30.0	41.0
28	36.186828495062436	38.0	35.0	40.0	29.0	41.0
29	36.120423033549706	38.0	35.0	40.0	29.0	41.0
30	36.008675933159886	38.0	35.0	40.0	29.0	41.0
31	36.13959317513651	38.0	35.0	40.0	30.0	41.0
32	36.09741359946832	38.0	35.0	40.0	30.0	41.0
33	36.063492735346514	38.0	35.0	40.0	29.0	41.0
34	36.096011656595664	38.0	35.0	40.0	29.0	41.0
35	35.872800049653996	38.0	34.0	40.0	28.0	41.0
36	35.823215768487174	38.0	34.0	40.0	28.0	41.0
37	35.7249723497313	38.0	34.0	40.0	27.0	41.0
38	35.61080354997616	38.0	34.0	40.0	27.0	41.0
39	35.523844144074125	38.0	34.0	40.0	27.0	41.0
40	35.30093042553937	38.0	34.0	40.0	26.0	41.0
41	35.20199003683324	38.0	34.0	40.0	26.0	41.0
42	34.97043488684064	38.0	33.0	40.0	25.0	41.0
43	34.59297950830095	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	7.0
14	10.0
15	45.0
16	120.0
17	272.0
18	519.0
19	1027.0
20	2033.0
21	4033.0
22	7445.0
23	12801.0
24	20962.0
25	32473.0
26	48014.0
27	69199.0
28	96272.0
29	128309.0
30	165876.0
31	207887.0
32	256663.0
33	312768.0
34	385376.0
35	468044.0
36	581649.0
37	754080.0
38	996971.0
39	1070050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88597134117897	19.23160441273212	12.61510274660225	25.267321499486656
2	18.305891734726014	22.81277437967629	33.44975582932044	25.431578056277253
3	20.24015674443362	24.421727464653095	29.928768212763817	25.409347578149465
4	14.580650485131278	15.673091749095796	34.07902063539686	35.66723713037606
5	14.351783266710072	37.851212654797486	33.025456031582216	14.77154804691022
6	34.39195462990611	35.040355431554936	15.726035855804938	14.841654082734015
7	28.45230877742863	31.60421710391646	21.26990806007778	18.673566058577133
8	28.57478981972149	32.52482566414442	19.951729629012448	18.948654887121634
9	27.908337869832906	13.106627510198898	19.391076970629626	39.59395764933858
10	17.20304660699769	26.725356314549526	32.07550324022557	23.99609383822721
11	36.490031586841766	22.163626633962373	20.68919461823244	20.657147160963422
12	21.808223534037797	25.89450553281032	28.94820849912083	23.349062434031058
13	30.344549291283464	19.06423558900363	25.112561802952243	25.478653316760656
14	23.095190373811715	20.872551601830438	25.134667790402514	30.89759023395533
15	25.656141454132918	27.599814402184208	22.403893641567507	24.340150502115364
16	24.54701843938131	25.813088629715107	24.93985766447308	24.7000352664305
17	23.925827742960898	26.138525045123423	25.622226636701168	24.31342057521451
18	23.91490813210453	24.269599856729016	27.4642440543697	24.351247956796758
19	24.084055394083244	25.478955651263195	27.459317780416587	22.977671174236974
20	23.989638107378898	24.58710443754134	26.953031979343084	24.47022547573668
21	24.948091833571613	25.327752831229233	26.812891045225506	22.911264289973644
22	24.867475227689003	24.86928923470423	26.19358549335033	24.069650044256434
23	23.317335095647078	25.067264980719063	26.861424625074093	24.753975298559766
24	23.456764654464564	25.997156988613195	26.67819213215361	23.867886224768633
25	24.156989146724893	24.91894323065046	26.180745169183723	24.743322453440932
26	23.583229398437926	26.05742826091325	26.838802890531255	23.520539450117564
27	24.61726675026512	25.444169399088835	26.024989547229183	23.91357430341686
28	23.319487005929844	26.126716215141943	27.029184705217634	23.524612073710575
29	23.334621515439245	26.1717818404026	27.0855078446022	23.40808879955596
30	23.348902374588537	26.603391019349232	26.943517334704392	23.104189271357843
31	23.844553114925702	26.23651699271072	26.100181916448616	23.818747975914967
32	23.05304138728143	26.269702650459877	26.140979289908735	24.536276672349963
33	22.733936212043904	25.807201999106866	26.91641393577099	24.542447853078237
34	23.804040291585622	25.95278495427013	26.970780793080312	23.27239396106394
35	23.80275981604546	25.834910067045342	27.30598083448235	23.05634928242684
36	22.75296550132129	26.377173673920858	26.976418442333518	23.893442382424336
37	23.12782471570331	25.975353335665456	27.222323099191858	23.674498849439374
38	22.738489013964475	25.403603222601244	27.69008792776553	24.167819835668748
39	23.08064274892489	25.005339760846297	27.59198927388323	24.32202821634559
40	23.096488633734378	25.267108086896627	28.144745666435057	23.491657612933942
41	21.95843042865164	25.44223090139609	28.770702577366063	23.828636092586205
42	22.798902561324553	25.44986040148955	28.48515653191025	23.26608050527565
43	21.932091758165832	24.51050710210423	28.606783923843004	24.95061721588693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1562.0
1	1586.5
2	1611.0
3	3342.5
4	5074.0
5	5074.0
6	7813.5
7	10553.0
8	10139.0
9	9725.0
10	13227.0
11	16729.0
12	16729.0
13	30000.5
14	43272.0
15	67624.5
16	91977.0
17	80711.0
18	69445.0
19	69445.0
20	71822.5
21	74200.0
22	59513.0
23	44826.0
24	52598.0
25	60370.0
26	60370.0
27	68171.0
28	75972.0
29	84140.5
30	92309.0
31	101481.5
32	110654.0
33	110654.0
34	127078.5
35	143503.0
36	170885.0
37	198267.0
38	226192.5
39	254118.0
40	254118.0
41	279579.5
42	305041.0
43	322504.5
44	339968.0
45	372526.5
46	405085.0
47	405085.0
48	445341.0
49	485597.0
50	487872.5
51	490148.0
52	466042.0
53	441936.0
54	441936.0
55	426506.0
56	411076.0
57	395313.0
58	379550.0
59	354867.5
60	330185.0
61	330185.0
62	278853.0
63	227521.0
64	200676.5
65	173832.0
66	147189.0
67	120546.0
68	120546.0
69	100305.5
70	80065.0
71	68018.0
72	55971.0
73	41717.5
74	27464.0
75	27464.0
76	21563.0
77	15662.0
78	13798.5
79	11935.0
80	9559.5
81	7184.0
82	7184.0
83	5911.0
84	4638.0
85	4013.0
86	3388.0
87	2229.0
88	1070.0
89	1070.0
90	773.5
91	477.0
92	340.0
93	203.0
94	192.0
95	181.0
96	181.0
97	95.0
98	9.0
99	10.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5622911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.23538951300212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.74661643384108	36.526046462417796
2	9.657053550990375	8.736811578539399
3	3.2839180035895414	4.456479300633996
4	1.7071531211924977	3.0889494558191983
5	0.9842077730088814	2.2260510986890565
6	0.6490918427456073	1.761715340178592
7	0.470518170623939	1.4898850904783352
8	0.3461271508715959	1.2525757192561837
9	0.26080994574662375	1.061805553624213
>10	1.6768348570041107	14.138063629219433
>50	0.11888942993466622	3.691350809923457
>100	0.07659503410178004	6.877373387954178
>500	0.011603696690945913	3.674852875920983
>1k	0.010266313386137324	8.480365747966477
>5k	1.5733813618572156E-4	0.4498281116682972
>10k+	1.5733813618572156E-4	2.0878458377104545
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44534	0.7920096903543379	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29689	0.5280005321087244	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27577	0.4904399162640134	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15540	0.27636930408466365	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7909	0.14065668121014185	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	7316	0.1301105423863191	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7784382502230604E-5
2	0.0	0.0	0.0	0.0	1.7784382502230604E-5
3	0.0	0.0	0.0	0.0	1.7784382502230604E-5
4	0.0	0.0	0.0	0.0	1.7784382502230604E-5
5	0.0	0.0	0.0	0.0	1.7784382502230604E-5
6	0.0	0.0	0.0	0.0	1.7784382502230604E-5
7	0.0	0.0	0.0	0.0	1.7784382502230604E-5
8	0.0	0.0	0.0	1.7784382502230604E-5	3.556876500446121E-5
9	0.0	0.0	0.0	3.556876500446121E-5	8.892191251115304E-5
10	0.0	0.0	0.0	3.556876500446121E-5	8.892191251115304E-5
11	0.0	0.0	0.0	3.556876500446121E-5	8.892191251115304E-5
12	0.0	0.0	0.0	5.335314750669182E-5	1.2449067751561424E-4
13	0.0	0.0	0.0	7.113753000892242E-5	1.9562820752453666E-4
14	0.0	0.0	0.0	8.892191251115304E-5	2.489813550312285E-4
15	0.0	1.7784382502230604E-5	0.0	2.667657375334591E-4	3.379032675423815E-4
16	0.0	1.7784382502230604E-5	0.0	6.046690050758406E-4	3.7347203254684273E-4
17	0.0	1.7784382502230604E-5	0.0	0.0011382004801427587	3.7347203254684273E-4
18	0.0	1.7784382502230604E-5	0.0	0.0014049662176762179	4.090407975513039E-4
19	0.0	1.7784382502230604E-5	0.0	0.001849575780231983	4.090407975513039E-4
20	0.0	1.7784382502230604E-5	0.0	0.0022764009602855174	4.4460956255576515E-4
21	0.0	1.7784382502230604E-5	0.0	0.00352130773544166	4.4460956255576515E-4
22	0.0	1.7784382502230604E-5	0.0	0.005406452280678104	4.4460956255576515E-4
23	0.0	1.7784382502230604E-5	0.0	0.00823416909853277	4.6239394505799574E-4
24	0.0	1.7784382502230604E-5	0.0	0.012182302014027965	4.6239394505799574E-4
25	0.0	1.7784382502230604E-5	0.0	0.013818465204233181	4.6239394505799574E-4
26	0.0	1.7784382502230604E-5	0.0	0.018086717004768528	4.6239394505799574E-4
27	0.0	1.7784382502230604E-5	0.0	0.04398077792801629	4.6239394505799574E-4
28	0.0	1.7784382502230604E-5	0.0	0.08755251505848127	4.6239394505799574E-4
29	0.0	1.7784382502230604E-5	0.0	0.14709462767594933	4.801783275602264E-4
30	0.0	1.7784382502230604E-5	0.0	0.23944892601003287	4.801783275602264E-4
31	1.7784382502230604E-5	1.7784382502230604E-5	0.0	0.5144132638770202	4.801783275602264E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1930	0.0	17.829014	5
CTTATAC	3420	0.0	17.418129	37
CAAGACG	2025	0.0	16.809877	4
TATACCG	205	5.456968E-12	15.341464	5
TCTAGCG	1265	0.0	15.209485	28
CTAGCGG	1300	0.0	14.8	29
GACGGAC	2265	0.0	14.375276	7
GCGTTAT	1560	0.0	14.3493595	1
ACGGACC	2265	0.0	14.293598	8
TCTTATA	5585	0.0	14.210384	37
CGGTCCA	1525	0.0	14.072131	10
CGGACCA	2335	0.0	13.944326	9
GGGTATA	280	0.0	13.875	1
AGACGGA	2425	0.0	13.731958	6
CGCAAGA	2390	0.0	13.700837	2
TAGCGGC	1435	0.0	13.665504	30
CGCCGGT	1500	0.0	13.566666	7
CGCAATA	1395	0.0	13.526881	36
ATACCGT	1570	0.0	13.433121	6
CGTTATT	1595	0.0	13.338557	2
>>END_MODULE