FastQCFastQC Report
Thu 9 Feb 2017
SRR2727241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5860612
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT347750.5933680646321579No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT235470.40178397750951605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221390.37775918282937004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114560.19547446580664274No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT88000.1501549667509127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC35800.019.22346537
AAGACGG21300.018.55
CAAGACG22050.018.038554
CGAACGA12500.015.98416
ACGAACG12500.015.98415
GACGGAC24900.015.5281127
TAACGAA13350.015.10486813
ACGGACC25400.015.0767728
CGCAAGA27050.014.9094282
TCTTATA59300.014.849915537
AGACGGA26450.014.8279776
AACGAAC13600.014.82720614
CGGACCA25850.014.8143149
GCGCAAG27900.014.6541211
TCTAGCG12000.014.64583328
ATACCGT16450.014.3951386
CGAGCCG19250.014.22337615
TACCGTC16100.014.1335417
GAGCGAA27700.014.0252716
CTAGCGG12550.014.003983529
Produced by FastQC (version 0.11.5)