##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727241.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5860612
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231844728844017	31.0	31.0	34.0	30.0	34.0
2	31.458192591490445	31.0	31.0	34.0	30.0	34.0
3	31.60799025767275	31.0	31.0	34.0	30.0	34.0
4	35.41969063981714	37.0	35.0	37.0	33.0	37.0
5	35.23386226557909	37.0	35.0	37.0	33.0	37.0
6	35.23194574218529	37.0	35.0	37.0	32.0	37.0
7	35.13379285303309	37.0	35.0	37.0	32.0	37.0
8	35.13646714711706	37.0	35.0	37.0	32.0	37.0
9	36.71736296482347	39.0	35.0	39.0	32.0	39.0
10	36.452918227652674	38.0	35.0	39.0	32.0	39.0
11	36.60693371272488	39.0	35.0	39.0	32.0	39.0
12	36.45269726096865	38.0	35.0	39.0	32.0	39.0
13	36.521435986548845	38.0	35.0	39.0	32.0	39.0
14	37.54074847473267	39.0	36.0	41.0	32.0	41.0
15	37.53665589873549	39.0	36.0	41.0	32.0	41.0
16	37.48398409585893	39.0	36.0	41.0	32.0	41.0
17	37.45824343942237	39.0	36.0	41.0	32.0	41.0
18	37.39023467173735	39.0	36.0	41.0	32.0	41.0
19	37.399811828525756	39.0	36.0	41.0	32.0	41.0
20	37.3119088927914	39.0	36.0	41.0	31.0	41.0
21	37.20987159702775	39.0	36.0	40.0	31.0	41.0
22	37.127255310537535	39.0	36.0	40.0	31.0	41.0
23	37.014217286522296	39.0	36.0	40.0	31.0	41.0
24	36.877972812395704	39.0	36.0	40.0	30.0	41.0
25	36.75310121195534	39.0	35.0	40.0	30.0	41.0
26	36.532424600024704	38.0	35.0	40.0	30.0	41.0
27	36.36090770042446	38.0	35.0	40.0	30.0	41.0
28	36.12975231938234	38.0	35.0	40.0	29.0	41.0
29	36.076660082598885	38.0	35.0	40.0	29.0	41.0
30	35.97948303009993	38.0	34.0	40.0	29.0	41.0
31	36.129003933377604	38.0	35.0	40.0	30.0	41.0
32	36.10273176248487	38.0	35.0	40.0	29.0	41.0
33	36.09311672569349	38.0	35.0	40.0	29.0	41.0
34	36.14495687481103	38.0	35.0	40.0	29.0	41.0
35	35.92948176743316	38.0	35.0	40.0	28.0	41.0
36	35.88206760659126	38.0	34.0	40.0	28.0	41.0
37	35.80595149448556	38.0	34.0	40.0	27.0	41.0
38	35.70329446822277	38.0	34.0	40.0	27.0	41.0
39	35.62813525276882	38.0	34.0	40.0	27.0	41.0
40	35.4314875306538	38.0	34.0	40.0	26.0	41.0
41	35.34886117695558	38.0	34.0	40.0	26.0	41.0
42	35.13674732263456	38.0	33.0	40.0	25.0	41.0
43	34.78022755985211	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	5.0
14	12.0
15	61.0
16	151.0
17	307.0
18	487.0
19	1021.0
20	2204.0
21	4218.0
22	7760.0
23	13439.0
24	22081.0
25	34111.0
26	50751.0
27	72471.0
28	99842.0
29	133855.0
30	173085.0
31	217323.0
32	267180.0
33	326019.0
34	400111.0
35	486312.0
36	603104.0
37	774332.0
38	1041464.0
39	1128897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35335831820977	19.175676533440537	12.583429853400977	25.887535294948723
2	18.409749698495652	22.375291181194047	33.76840166180597	25.446557458504333
3	19.99990785945222	23.970073432603968	30.059130343383934	25.97088836455988
4	14.625605653471002	15.531159544429832	34.37509597973727	35.468138822361894
5	14.518278978372907	37.53073911052292	33.212179888380255	14.738802022723906
6	34.63761122558531	35.167504690636406	15.592996089828162	14.601887993950118
7	28.766876223848293	31.653741964149816	21.088872629684406	18.490509182317478
8	28.183165853668523	33.06115470534476	19.88749639116188	18.86818304982483
9	28.127403759197843	13.270508267737227	19.04575836107219	39.556329611992744
10	17.125941795839754	26.37784245058366	32.06530307756255	24.430912676014042
11	37.07996707511093	21.80937758718714	20.630507530612842	20.48014780708909
12	21.936139092640836	25.37299858786079	28.8222799939665	23.86858232553187
13	30.29983899292429	18.847604994154192	24.963621546691712	25.888934466229806
14	23.010583195065635	20.62733380063379	24.821230274244396	31.54085273005618
15	25.880829510638137	27.53128854119672	21.906244603805884	24.68163734435926
16	24.88905595524836	25.818003307504405	24.46481698498382	24.828123752263416
17	24.22267503803357	25.9122255491406	25.045711949537008	24.819387463288816
18	24.296694611416008	24.459339741310295	26.740978587219217	24.50298706005448
19	24.752107800345765	25.245042667898847	26.6325769390637	23.370272592691684
20	24.345443786416844	24.346501696409863	26.113023691041143	25.195030826132154
21	25.403183148790603	25.218458413558174	26.157507099941096	23.220851337710123
22	25.488396092421745	24.34527315577281	25.466333550148008	24.699997201657435
23	23.68254373434037	24.705610949846193	26.359175458126217	25.252669857687216
24	23.900473192902037	25.399497526879443	26.180866435109508	24.519162845109012
25	24.610450239667802	24.437396640487375	25.586594028063963	25.36555909178086
26	23.977171667395826	25.7159832454358	26.284899938777723	24.021945148390646
27	25.296538996268648	25.07512867256867	25.30401261847739	24.3243197126853
28	23.71025415093168	25.943962848931136	26.134079512515072	24.21170348762211
29	23.841725062160744	25.826534839706163	26.4635331600181	23.868206938114994
30	24.152409338819904	26.07526312951617	26.14754227032945	23.624785261334484
31	24.367455139497377	25.770994565072726	25.536172672751583	24.325377622678314
32	23.664098561720177	25.758009573061653	25.411253978253466	25.166637886964704
33	23.224809968651737	25.199671979649906	26.275378748840566	25.300139302857787
34	24.497492753316546	25.397825346567902	26.548473094618785	23.556208805496766
35	24.559875316775788	25.186311600221956	26.62566639798028	23.628146685021974
36	23.217063337412544	25.89900167422788	26.49581647786955	24.38811851049003
37	23.647427947797944	25.468841820615324	26.815032286730467	24.06869794485627
38	23.39847783815069	24.78720652382379	27.152642079018367	24.66167355900715
39	23.511657144339193	24.580692255348076	27.155986439641456	24.751664160671275
40	23.88143081302772	24.608436798068187	27.645832892537502	23.86429949636659
41	22.530428562750785	24.97556569177417	28.250547894997997	24.24345785047705
42	23.361485114523877	24.943674824404006	28.064850565094567	23.62998949597755
43	22.44263909639471	23.963470026679808	28.059748708837915	25.534142168087566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1281.0
1	1390.0
2	1499.0
3	2747.5
4	3996.0
5	3996.0
6	6306.5
7	8617.0
8	8230.5
9	7844.0
10	10540.5
11	13237.0
12	13237.0
13	23101.5
14	32966.0
15	52224.5
16	71483.0
17	63340.0
18	55197.0
19	55197.0
20	57716.5
21	60236.0
22	49968.0
23	39700.0
24	46652.0
25	53604.0
26	53604.0
27	62107.5
28	70611.0
29	79076.0
30	87541.0
31	97883.5
32	108226.0
33	108226.0
34	126837.5
35	145449.0
36	175490.0
37	205531.0
38	236986.5
39	268442.0
40	268442.0
41	294188.5
42	319935.0
43	338702.0
44	357469.0
45	392356.5
46	427244.0
47	427244.0
48	470359.5
49	513475.0
50	521045.5
51	528616.0
52	508769.0
53	488922.0
54	488922.0
55	468819.0
56	448716.0
57	430688.0
58	412660.0
59	384211.5
60	355763.0
61	355763.0
62	298553.0
63	241343.0
64	212863.5
65	184384.0
66	155989.5
67	127595.0
68	127595.0
69	106105.5
70	84616.0
71	71893.0
72	59170.0
73	43895.0
74	28620.0
75	28620.0
76	22477.5
77	16335.0
78	14361.5
79	12388.0
80	9819.0
81	7250.0
82	7250.0
83	6043.5
84	4837.0
85	4280.5
86	3724.0
87	2431.5
88	1139.0
89	1139.0
90	812.5
91	486.0
92	346.5
93	207.0
94	222.0
95	237.0
96	237.0
97	121.5
98	6.0
99	10.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5860612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.08214654043163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32638224948474	34.17550824594596
2	10.217764308072027	8.804064384719021
3	3.5447428943554398	4.581453984683245
4	1.8046087043876848	3.1098566660227487
5	1.0788320040696844	2.323919924591884
6	0.7201042007739564	1.8614180821274393
7	0.49172352362022653	1.4829153431388795
8	0.39683127242138433	1.367707442422722
9	0.2938776971903798	1.1394793813788484
>10	1.8785859907557119	15.047100681211306
>50	0.1366729712252907	4.047967360342689
>100	0.08614145092191651	7.318197131027768
>500	0.01232201014378913	3.7297162248042435
>1k	0.010816414110212596	8.39074866734614
>5k	4.3582620956726014E-4	1.0509793907971443
>10k+	1.5848225802445824E-4	1.5689670894398517
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34775	0.5933680646321579	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23547	0.40178397750951605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22139	0.37775918282937004	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11456	0.19547446580664274	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	8800	0.1501549667509127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.706306440351281E-5
2	0.0	0.0	0.0	0.0	3.412612880702562E-5
3	0.0	0.0	0.0	0.0	3.412612880702562E-5
4	0.0	0.0	0.0	1.706306440351281E-5	3.412612880702562E-5
5	0.0	0.0	0.0	1.706306440351281E-5	3.412612880702562E-5
6	0.0	0.0	0.0	1.706306440351281E-5	5.118919321053842E-5
7	0.0	0.0	0.0	3.412612880702562E-5	5.118919321053842E-5
8	0.0	0.0	0.0	3.412612880702562E-5	6.825225761405124E-5
9	0.0	0.0	0.0	3.412612880702562E-5	1.5356757963161526E-4
10	0.0	0.0	0.0	3.412612880702562E-5	1.5356757963161526E-4
11	0.0	0.0	0.0	3.412612880702562E-5	1.7063064403512808E-4
12	0.0	1.706306440351281E-5	0.0	5.118919321053842E-5	1.8769370843864087E-4
13	0.0	1.706306440351281E-5	0.0	1.0237838642107685E-4	1.8769370843864087E-4
14	0.0	1.706306440351281E-5	0.0	1.1944145082458965E-4	2.2181983724566648E-4
15	0.0	1.706306440351281E-5	0.0	2.388829016491793E-4	2.388829016491793E-4
16	0.0	1.706306440351281E-5	0.0	3.9245048128079454E-4	3.071351592632305E-4
17	0.0	1.706306440351281E-5	0.0	5.972072541229483E-4	3.241982236667433E-4
18	0.0	1.706306440351281E-5	0.0	7.166487049475379E-4	3.241982236667433E-4
19	0.0	1.706306440351281E-5	0.0	8.872793489826659E-4	3.241982236667433E-4
20	0.0	1.706306440351281E-5	0.0	0.001160288379438871	3.241982236667433E-4
21	0.0	1.706306440351281E-5	0.0	0.0021328830504391007	3.241982236667433E-4
22	0.0	1.706306440351281E-5	0.0	0.003378486751895536	3.241982236667433E-4
23	0.0	1.706306440351281E-5	0.0	0.005170108514264381	4.948288677018714E-4
24	0.0	1.706306440351281E-5	0.0	0.00820733397808966	5.28954996508897E-4
25	0.0	1.706306440351281E-5	0.0	0.009640631387984737	5.630811253159227E-4
26	0.0	1.706306440351281E-5	0.0	0.01330919023473999	5.630811253159227E-4
27	0.0	1.706306440351281E-5	0.0	0.03480865138316613	5.630811253159227E-4
28	0.0	1.706306440351281E-5	0.0	0.07599888885324604	5.630811253159227E-4
29	0.0	1.706306440351281E-5	0.0	0.1303106228496273	5.630811253159227E-4
30	0.0	1.706306440351281E-5	0.0	0.2159330800264546	5.630811253159227E-4
31	0.0	1.706306440351281E-5	0.0	0.5057662919845232	5.801441897194355E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3580	0.0	19.223465	37
AAGACGG	2130	0.0	18.5	5
CAAGACG	2205	0.0	18.03855	4
CGAACGA	1250	0.0	15.984	16
ACGAACG	1250	0.0	15.984	15
GACGGAC	2490	0.0	15.528112	7
TAACGAA	1335	0.0	15.104868	13
ACGGACC	2540	0.0	15.076772	8
CGCAAGA	2705	0.0	14.909428	2
TCTTATA	5930	0.0	14.8499155	37
AGACGGA	2645	0.0	14.827977	6
AACGAAC	1360	0.0	14.827206	14
CGGACCA	2585	0.0	14.814314	9
GCGCAAG	2790	0.0	14.654121	1
TCTAGCG	1200	0.0	14.645833	28
ATACCGT	1645	0.0	14.395138	6
CGAGCCG	1925	0.0	14.223376	15
TACCGTC	1610	0.0	14.133541	7
GAGCGAA	2770	0.0	14.02527	16
CTAGCGG	1255	0.0	14.0039835	29
>>END_MODULE