FastQCFastQC Report
Thu 9 Feb 2017
SRR2727240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5052390
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG82370.16303175328903746No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC78130.15463968537662373No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT71240.14100257501895144No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT60960.12065576885394833No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC58010.11481694801866048No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG55730.11030423225443799No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG55030.10891874934436968No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA53670.10622695397623698No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC52340.10359453644710721No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA50650.1002495848499423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15350.019.7654725
CAAGACG16400.018.9512214
TATACTG3650.017.7397255
CTTATAC13450.017.19330837
TAGAGTG8450.016.6390515
TTAGAGT8850.016.3050864
ACGGACC18900.015.8571438
ATTAGAG9450.015.6613763
TCTAACG957.067262E-515.5789482
CGGACCA19450.015.3136249
CGCAAGA20350.015.1818182
GACGGAC20150.015.1488847
AATTAGA8800.015.1363642
TCTAGCG12050.015.04564428
AGACGGA21150.014.7825066
TCGTTTA13050.014.60153230
AAATTAG8750.014.5885721
GCAAGAC24000.014.4916663
GCGCAAG21850.014.4782611
TCTTATA21800.014.341742537
Produced by FastQC (version 0.11.5)