FastQCFastQC Report
Thu 9 Feb 2017
SRR2727239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4854374
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72920.15021504317549494No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT60400.12442387010147961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.11494787999441329No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC52550.10825288698398598No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC51380.10584268950023218No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG49940.10287629259715053No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG49470.10190809360795026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG16150.018.5572765
CAAGACG16300.018.5000024
CTAGTAC2650.016.0566043
TAACGAA10500.015.68095213
ACGAACG10500.015.68095215
GACGGAC18300.015.6693987
ACGGACC18450.015.6422778
CGAACGA10950.015.20547916
ATTATAC4750.015.1894733
TAGGTCG1101.4537074E-515.13636421
AACGAAC11100.015.00000114
ATAACGA11150.014.93273512
GTCTTAC3500.014.81
CGGACCA19600.014.724499
GTATTAC3150.014.6825391
CTTATAC15000.014.55333237
TAGAGTG9700.014.4948455
GATAACG11700.014.38888811
TCGTTTA11750.014.3276630
AGACGGA20700.014.2101456
Produced by FastQC (version 0.11.5)