FastQCFastQC Report
Thu 9 Feb 2017
SRR2727238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4909835
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131270.267361326806298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86820.1768287529010649No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT85510.17416063879947086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82930.16890587972915588No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT54060.11010553307799549No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG52830.10760035724214764No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG49180.10016629886747722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC22000.018.58409137
CAAGACG19900.017.756284
AAGACGG19650.017.6055985
TCGTTAC11800.015.20762723
TACGGAT22250.014.88314627
GACGGAC23000.014.8804347
CGCAAGA23950.014.5991642
TATACTG4950.014.5757585
ACGGATG23200.014.51293128
TACTTAC11000.014.46363631
GTTACGG19650.014.40458125
ACGGACC23600.014.3453398
AGACGGA24400.014.3299186
GCGCAAG24900.014.265061
TCTAGCG12850.014.25291828
TCTTATA36500.014.14109537
CCCTATA2500.014.0599992
TATACCG1458.9263085E-714.0344835
CTAGCGG12950.014.029
CGGATGG24250.013.96082429
Produced by FastQC (version 0.11.5)