##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727238.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4909835
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230605101800773	31.0	31.0	34.0	30.0	34.0
2	31.474420016151257	31.0	31.0	34.0	30.0	34.0
3	31.631555439235736	31.0	31.0	34.0	30.0	34.0
4	35.43694889950477	37.0	35.0	37.0	33.0	37.0
5	35.24550906496858	37.0	35.0	37.0	33.0	37.0
6	35.24168775529117	37.0	35.0	37.0	32.0	37.0
7	35.14629860270254	37.0	35.0	37.0	32.0	37.0
8	35.14376226492336	37.0	35.0	37.0	32.0	37.0
9	36.73353014918017	39.0	35.0	39.0	32.0	39.0
10	36.461903098576634	38.0	35.0	39.0	32.0	39.0
11	36.61651420057904	39.0	35.0	39.0	32.0	39.0
12	36.46055315504493	38.0	35.0	39.0	32.0	39.0
13	36.5240076295843	38.0	35.0	39.0	32.0	39.0
14	37.56173578134499	39.0	36.0	41.0	32.0	41.0
15	37.55997502971078	39.0	36.0	41.0	32.0	41.0
16	37.50038280308809	39.0	36.0	41.0	32.0	41.0
17	37.4465610351468	39.0	36.0	41.0	32.0	41.0
18	37.36213538744174	39.0	36.0	41.0	32.0	41.0
19	37.36088850236312	39.0	36.0	41.0	31.0	41.0
20	37.26538447829713	39.0	36.0	40.0	31.0	41.0
21	37.15349640059188	39.0	36.0	40.0	31.0	41.0
22	37.0826266055784	39.0	36.0	40.0	31.0	41.0
23	36.96306128413684	39.0	36.0	40.0	31.0	41.0
24	36.82311055259495	39.0	36.0	40.0	30.0	41.0
25	36.69217926875343	39.0	35.0	40.0	30.0	41.0
26	36.46951577802513	38.0	35.0	40.0	30.0	41.0
27	36.306947789487836	38.0	35.0	40.0	30.0	41.0
28	36.05156670234336	38.0	35.0	40.0	29.0	41.0
29	35.99805451710699	38.0	34.0	40.0	29.0	41.0
30	35.91259706283409	38.0	34.0	40.0	28.0	41.0
31	36.100847991836794	38.0	35.0	40.0	29.0	41.0
32	36.09322573976519	38.0	35.0	40.0	29.0	41.0
33	36.106510096571476	38.0	35.0	40.0	29.0	41.0
34	36.16735083765544	38.0	35.0	40.0	29.0	41.0
35	35.961412959905985	38.0	35.0	40.0	28.0	41.0
36	35.919416029255565	38.0	34.0	40.0	28.0	41.0
37	35.85887794600022	38.0	34.0	40.0	28.0	41.0
38	35.76222989163587	38.0	34.0	40.0	27.0	41.0
39	35.70187918738613	38.0	34.0	40.0	27.0	41.0
40	35.51718642276166	38.0	34.0	40.0	27.0	41.0
41	35.449718778736965	38.0	34.0	40.0	26.0	41.0
42	35.26232673806757	38.0	33.0	40.0	26.0	41.0
43	34.90284826272166	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	8.0
14	17.0
15	44.0
16	142.0
17	234.0
18	450.0
19	882.0
20	1782.0
21	3607.0
22	6645.0
23	11298.0
24	18682.0
25	28535.0
26	42249.0
27	59781.0
28	83162.0
29	111901.0
30	143877.0
31	181775.0
32	223245.0
33	273335.0
34	336467.0
35	407967.0
36	504994.0
37	641142.0
38	890761.0
39	936850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08933701438032	19.081598465121534	12.32088247364728	26.50818204685086
2	18.84611193655184	21.94338506283816	33.41678488177301	25.79371811883699
3	20.130778325544547	23.363575354365267	29.778454876793216	26.727191443296977
4	15.015942490939105	15.376402669336139	34.18929556695897	35.418359272765784
5	14.956673696773926	36.86822469594192	33.23818417523196	14.936917432052196
6	35.33330549804627	34.70259183862594	15.373388311419834	14.590714351907957
7	29.27318331471424	31.220926976161113	20.873063962434582	18.632825746690063
8	27.9410000539733	33.006791470589135	19.93380632954061	19.118402145896958
9	28.306531685891684	13.232766477895897	18.65372257927201	39.80697925694041
10	17.245100904612883	25.763554172390723	32.20301700566312	24.788327917333273
11	37.784935746313266	21.19024366399278	20.441277558207148	20.5835430314868
12	22.168525011532974	24.707877148621087	28.45274841211568	24.67084942773026
13	30.507074066643785	18.46127212014253	24.48418327703477	26.547470536178913
14	23.314856812907156	20.366570363362516	23.80860049268458	32.50997233104575
15	26.374246792407487	27.19238019200238	21.148144489580606	25.28522852600953
16	25.67933545628315	25.44784091522424	23.79642085732005	25.076402771172557
17	24.906600730981797	25.329914345390424	24.229083054725873	25.534401868901906
18	25.05377471951705	24.200935469318217	25.774185894230662	24.971103916934073
19	25.535847946010403	24.981715271490792	25.350444566874447	24.131992215624354
20	25.04249124461413	23.850475626981353	24.90996133271281	26.197071795691706
21	25.97999729115133	25.00550425828974	25.08173899937574	23.932759451183188
22	26.15008447330715	23.955713379370184	24.33055285971932	25.56364928760335
23	24.291366206807357	24.21337987936458	25.341503329541627	26.15375058428644
24	24.583229375325242	24.93342444298026	25.13078341736535	25.352562764329146
25	25.54757950114413	23.78593170646264	24.37006538916277	26.296423403230456
26	24.79054795120406	25.1660595518994	25.17775037246669	24.865642124429844
27	26.352005719133125	24.411125832130814	24.127002231235878	25.109866217500183
28	24.419048705302725	25.452342084815477	24.867108568821557	25.26150064106024
29	24.616428046971027	25.198606470482204	25.41867496565567	24.766290516891097
30	25.203657556720337	25.36490533795942	25.048316287614554	24.383120817705688
31	25.442952767251853	25.091820804568787	24.322548517414535	25.14267791076482
32	24.62115325667767	24.98033029623195	24.219693737162245	26.178822709928134
33	24.080422254515682	24.39864068751801	25.146058879779055	26.37487817818725
34	25.493097018535245	24.72881471576947	25.44702622389551	24.331062041799775
35	25.622999550901405	24.464488114162698	25.54717215547977	24.365340179456133
36	24.15753278878007	25.222313988148276	25.480958117737153	25.1391951053345
37	24.660523215138593	24.83120104850774	25.844941836130946	24.663333900222717
38	24.53127243583542	24.006651954699088	26.071263087252422	25.390812522213068
39	24.53776959918205	23.83833672618326	26.05749480379687	25.56639887083782
40	25.093755696474524	23.847379799932177	26.506206420378685	24.55265808321461
41	23.51958874381726	24.419415316400652	27.084637263777704	24.976358676004388
42	24.626346913898328	24.34588942398268	26.890496320141104	24.137267341977886
43	23.60621079934458	23.22528150131318	26.85959915149898	26.308908547843256
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	421.0
1	477.5
2	534.0
3	1034.0
4	1534.0
5	1534.0
6	2305.0
7	3076.0
8	3078.0
9	3080.0
10	4048.0
11	5016.0
12	5016.0
13	8892.0
14	12768.0
15	20336.5
16	27905.0
17	25401.5
18	22898.0
19	22898.0
20	25226.5
21	27555.0
22	24948.5
23	22342.0
24	27524.5
25	32707.0
26	32707.0
27	39374.0
28	46041.0
29	53487.0
30	60933.0
31	70151.0
32	79369.0
33	79369.0
34	95779.5
35	112190.0
36	138349.0
37	164508.0
38	191949.0
39	219390.0
40	219390.0
41	242485.5
42	265581.0
43	282229.5
44	298878.0
45	330739.5
46	362601.0
47	362601.0
48	410760.5
49	458920.0
50	468277.0
51	477634.0
52	455567.0
53	433500.0
54	433500.0
55	415053.5
56	396607.0
57	384569.0
58	372531.0
59	346280.0
60	320029.0
61	320029.0
62	267375.0
63	214721.0
64	189195.5
65	163670.0
66	138374.0
67	113078.0
68	113078.0
69	93506.5
70	73935.0
71	62925.0
72	51915.0
73	38012.5
74	24110.0
75	24110.0
76	18950.5
77	13791.0
78	12253.0
79	10715.0
80	8467.0
81	6219.0
82	6219.0
83	5220.0
84	4221.0
85	3663.0
86	3105.0
87	2049.0
88	993.0
89	993.0
90	697.0
91	401.0
92	296.5
93	192.0
94	195.0
95	198.0
96	198.0
97	104.0
98	10.0
99	11.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4909835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.913928830785906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.15870701735484	35.20083755127939
2	9.630542791373067	8.4582994148439
3	3.4967437966386266	4.606672746752422
4	1.7325607478517888	3.043341975047066
5	1.0613915786277355	2.330493712272694
6	0.7307377344219947	1.9253738918026322
7	0.5056198445139495	1.554262770719303
8	0.38690291289513157	1.3592341585040453
9	0.28035548485203343	1.1080359728201405
>10	1.7889063348033998	14.427624941791104
>50	0.11967268041059073	3.6265608183916775
>100	0.08200723586369657	7.47381182194222
>500	0.013576866319060077	4.198752110912502
>1k	0.011995997390398926	9.67921981611343
>5k	2.324805695813698E-4	0.7394476616043217
>10k+	4.6496113916273966E-5	0.26803063520309073
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13127	0.267361326806298	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8682	0.1768287529010649	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	8551	0.17416063879947086	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8293	0.16890587972915588	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	5406	0.11010553307799549	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	5283	0.10760035724214764	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	4918	0.10016629886747722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	2.0367283218275157E-5
5	0.0	0.0	0.0	0.0	2.0367283218275157E-5
6	0.0	0.0	0.0	0.0	8.146913287310063E-5
7	0.0	0.0	0.0	0.0	8.146913287310063E-5
8	0.0	0.0	0.0	2.0367283218275157E-5	1.0183641609137578E-4
9	0.0	0.0	0.0	2.0367283218275157E-5	1.4257098252792608E-4
10	0.0	0.0	0.0	2.0367283218275157E-5	1.4257098252792608E-4
11	0.0	0.0	0.0	2.0367283218275157E-5	1.4257098252792608E-4
12	0.0	0.0	0.0	2.0367283218275157E-5	1.4257098252792608E-4
13	0.0	0.0	0.0	2.0367283218275157E-5	1.4257098252792608E-4
14	0.0	0.0	0.0	6.110184965482547E-5	2.0367283218275157E-4
15	0.0	0.0	0.0	1.0183641609137578E-4	2.0367283218275157E-4
16	0.0	0.0	0.0	2.240401154010267E-4	2.240401154010267E-4
17	0.0	0.0	0.0	4.277129475837783E-4	2.444073986193019E-4
18	0.0	0.0	0.0	4.6844751402032856E-4	3.258765314924025E-4
19	0.0	0.0	0.0	6.721203462030801E-4	3.258765314924025E-4
20	0.0	0.0	0.0	0.0010183641609137577	3.258765314924025E-4
21	0.0	0.0	0.0	0.0017719536399899386	3.4624381471067765E-4
22	0.0	0.0	0.0	0.0032180307484874745	3.4624381471067765E-4
23	0.0	0.0	0.0	0.004969617105259138	4.480802308020534E-4
24	0.0	0.0	0.0	0.006884141727777003	4.480802308020534E-4
25	0.0	0.0	0.0	0.008085811437655237	4.480802308020534E-4
26	0.0	0.0	0.0	0.010529885423848256	4.480802308020534E-4
27	0.0	0.0	0.0	0.02393155778147331	4.480802308020534E-4
28	0.0	0.0	0.0	0.0489425815735152	4.480802308020534E-4
29	0.0	0.0	0.0	0.08169317298850165	4.6844751402032856E-4
30	0.0	0.0	0.0	0.13434260010774293	4.6844751402032856E-4
31	0.0	0.0	0.0	0.34630491656033247	4.888147972386038E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2200	0.0	18.584091	37
CAAGACG	1990	0.0	17.75628	4
AAGACGG	1965	0.0	17.605598	5
TCGTTAC	1180	0.0	15.207627	23
TACGGAT	2225	0.0	14.883146	27
GACGGAC	2300	0.0	14.880434	7
CGCAAGA	2395	0.0	14.599164	2
TATACTG	495	0.0	14.575758	5
ACGGATG	2320	0.0	14.512931	28
TACTTAC	1100	0.0	14.463636	31
GTTACGG	1965	0.0	14.404581	25
ACGGACC	2360	0.0	14.345339	8
AGACGGA	2440	0.0	14.329918	6
GCGCAAG	2490	0.0	14.26506	1
TCTAGCG	1285	0.0	14.252918	28
TCTTATA	3650	0.0	14.141095	37
CCCTATA	250	0.0	14.059999	2
TATACCG	145	8.9263085E-7	14.034483	5
CTAGCGG	1295	0.0	14.0	29
CGGATGG	2425	0.0	13.960824	29
>>END_MODULE