FastQCFastQC Report
Thu 9 Feb 2017
SRR2727237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7590323
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT316960.4175843373200323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT221690.29206925713174525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT218080.28731320129591326No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG120540.15880747103911125No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC117610.15494729275684316No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT112340.14800424171672272No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99620.1312460615971152No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT81590.10749213175776577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG27400.018.2974455
CAAGACG27550.018.063524
CTTATAC34100.017.252237
GCGCAAG32250.015.3736441
ACGGACC32100.015.2725868
GACGGAC32250.015.2589157
CGCAAGA32750.015.0259532
TCGTTTA20900.014.78229630
TCTTATA55400.014.62635437
AGACGGA34500.014.5855086
CGGACCA33550.014.4470939
CGCATCG22150.014.28216713
GTTACGG13200.014.15530325
ATACCGT20000.014.15256
TCTAGCG17150.014.13119528
CGAGCCG24750.014.052525515
GCAAGAC39350.013.9631523
ACCGTCG19200.013.8749998
CTAGCGG17750.013.65352229
TACCGTC19650.013.6514017
Produced by FastQC (version 0.11.5)