##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7590323
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23996897101744	31.0	31.0	34.0	30.0	34.0
2	31.474989799511825	31.0	31.0	34.0	30.0	34.0
3	31.632680980769855	31.0	31.0	34.0	30.0	34.0
4	35.43950922773642	37.0	35.0	37.0	33.0	37.0
5	35.25255249348414	37.0	35.0	37.0	33.0	37.0
6	35.256489743585355	37.0	35.0	37.0	32.0	37.0
7	35.15881919649533	37.0	35.0	37.0	32.0	37.0
8	35.159636157776156	37.0	35.0	37.0	32.0	37.0
9	36.746317251584685	39.0	35.0	39.0	32.0	39.0
10	36.4814099479034	38.0	35.0	39.0	32.0	39.0
11	36.62644330155647	39.0	35.0	39.0	32.0	39.0
12	36.47659961242756	38.0	35.0	39.0	32.0	39.0
13	36.53929233314577	38.0	35.0	39.0	32.0	39.0
14	37.579404064886305	39.0	36.0	41.0	32.0	41.0
15	37.579473100156605	39.0	36.0	41.0	32.0	41.0
16	37.52399706837245	39.0	36.0	41.0	32.0	41.0
17	37.487199951833404	39.0	36.0	41.0	32.0	41.0
18	37.41008215328913	39.0	36.0	41.0	32.0	41.0
19	37.41215597807893	39.0	36.0	41.0	32.0	41.0
20	37.32780265082263	39.0	36.0	41.0	31.0	41.0
21	37.218075172822026	39.0	36.0	40.0	31.0	41.0
22	37.15421675731059	39.0	36.0	40.0	31.0	41.0
23	37.045866954541985	39.0	36.0	40.0	31.0	41.0
24	36.90574182416216	39.0	36.0	40.0	31.0	41.0
25	36.78989537072401	39.0	35.0	40.0	30.0	41.0
26	36.574351842470996	39.0	35.0	40.0	30.0	41.0
27	36.406853305188726	38.0	35.0	40.0	30.0	41.0
28	36.19140305359864	38.0	35.0	40.0	29.0	41.0
29	36.14034382990025	38.0	35.0	40.0	29.0	41.0
30	36.052361144578434	38.0	35.0	40.0	29.0	41.0
31	36.22015861512086	38.0	35.0	40.0	30.0	41.0
32	36.19379399269307	38.0	35.0	40.0	30.0	41.0
33	36.21510599746546	38.0	35.0	40.0	30.0	41.0
34	36.26609012554538	38.0	35.0	40.0	30.0	41.0
35	36.07022744618378	38.0	35.0	40.0	29.0	41.0
36	36.04086980224689	38.0	35.0	40.0	29.0	41.0
37	35.96658284502517	38.0	35.0	40.0	28.0	41.0
38	35.87189135429414	38.0	34.0	40.0	28.0	41.0
39	35.82220532643999	38.0	34.0	40.0	28.0	41.0
40	35.63668067880642	38.0	34.0	40.0	27.0	41.0
41	35.57138411632812	38.0	34.0	40.0	27.0	41.0
42	35.378650948055835	38.0	34.0	40.0	26.0	41.0
43	35.011566569696704	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	8.0
14	14.0
15	74.0
16	160.0
17	382.0
18	637.0
19	1292.0
20	2609.0
21	5006.0
22	9164.0
23	16307.0
24	27095.0
25	42171.0
26	63461.0
27	89712.0
28	125106.0
29	168402.0
30	217099.0
31	273622.0
32	339277.0
33	415074.0
34	511315.0
35	624784.0
36	776462.0
37	995432.0
38	1382535.0
39	1503120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59953100810071	18.845469422052265	12.122066478593862	26.43293309125316
2	18.242649225862984	21.910187484775022	33.96296837433664	25.884194915025354
3	19.93988925108984	23.708345481476876	29.82834854327016	26.523416724163123
4	14.537813476448894	15.481053968322561	34.51002019281656	35.471112362411986
5	14.447184922169978	37.21767835176448	33.634998141712806	14.700138584352734
6	34.9640324924249	35.205115777022925	15.427867825914653	14.402983904637523
7	29.125941017266328	31.196472139591425	21.063517323307586	18.614069519834665
8	28.108000147029315	33.40158251499969	19.833227123536112	18.65719021443488
9	27.94903457995134	13.287102538324127	18.630524682546447	40.133338199178084
10	16.96602371203439	26.103948409046623	32.428119330363145	24.50190854855584
11	37.543053174416954	21.318315439277093	20.608819940864176	20.52981144544178
12	22.132654960796792	25.04471812332624	28.92792572858889	23.894701187288078
13	30.596365925402647	18.53657611145138	24.80654644077729	26.060511522368678
14	23.00381683361828	20.144188857312134	24.56442235725673	32.28757195181286
15	25.936616926578747	26.94275329258057	21.801417410036436	25.319212370804244
16	25.21772788852332	25.46123531238394	24.34037392084632	24.98066287824642
17	24.491026271214018	25.74460928737815	24.924775928507916	24.839588512899912
18	24.984470357849066	23.97842621453659	26.40747962899603	24.62962379861832
19	25.155793765298263	25.120143108534382	25.985903366694675	23.738159759472687
20	24.71728805216853	24.01374750455284	25.801998149485865	25.46696629379277
21	25.570479675239117	24.959978646495017	25.593245504835565	23.8762961734303
22	25.493776747050156	24.269494196755524	24.999199638803248	25.237529417391062
23	23.885531617033955	24.17082909383435	25.974177910478907	25.96946137865279
24	24.331006730543614	25.261019853832305	25.629778864483104	24.778194551140974
25	25.052873243997652	24.007647632386657	25.091303756111564	25.848175367504123
26	24.47690302507548	25.090579149266773	25.885894447443146	24.5466233782146
27	25.701528116787653	24.506901748449966	24.8321711737432	24.959398961019183
28	24.15165994912206	25.18815075458581	25.84388042511498	24.816308871177156
29	24.315421096045583	25.088642472790685	26.06267216823316	24.533264262930576
30	24.446443188254307	25.53067899745505	25.95789401847589	24.06498379581475
31	25.09208106163598	24.990293561947233	24.918096370865904	24.999529005550883
32	24.016922600000026	25.117639921252362	25.148863888928048	25.716573589819564
33	23.75475984355343	24.601008415583895	25.90974059997183	25.734491140890842
34	24.86873088273055	24.721424898518812	26.11058844267892	24.299255776071714
35	25.036866020062646	24.791303347696797	26.23867258349875	23.9331580487418
36	24.013470836484824	25.23127144918602	25.897949270406546	24.85730844392261
37	24.372954879522254	24.877478863547704	26.24531261713105	24.504253639798993
38	24.12878872216637	24.311824411161425	26.61927562239446	24.940111244277745
39	24.384706685077827	23.87176408698286	26.35072315104377	25.392806076895543
40	24.571128264238556	24.2036998952482	26.977956010567667	24.247215829945574
41	23.278350605106002	24.43274153155274	27.438740090507345	24.85016777283391
42	24.465243442209246	24.514793375723272	27.100388217998102	23.919574964069383
43	23.533412214473614	23.337004235524628	27.31583359496032	25.813749955041438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	626.0
1	730.5
2	835.0
3	2071.0
4	3307.0
5	3307.0
6	5304.5
7	7302.0
8	6875.0
9	6448.0
10	8511.5
11	10575.0
12	10575.0
13	18664.5
14	26754.0
15	43782.5
16	60811.0
17	55492.0
18	50173.0
19	50173.0
20	53796.0
21	57419.0
22	49236.0
23	41053.0
24	49281.0
25	57509.0
26	57509.0
27	68529.0
28	79549.0
29	91518.0
30	103487.0
31	118821.5
32	134156.0
33	134156.0
34	159247.0
35	184338.0
36	223557.0
37	262776.0
38	305443.0
39	348110.0
40	348110.0
41	381255.0
42	414400.0
43	438305.5
44	462211.0
45	510276.0
46	558341.0
47	558341.0
48	635055.5
49	711770.0
50	722108.5
51	732447.0
52	681431.5
53	630416.0
54	630416.0
55	610700.0
56	590984.0
57	568559.5
58	546135.0
59	514033.5
60	481932.0
61	481932.0
62	404489.5
63	327047.0
64	285916.5
65	244786.0
66	206417.5
67	168049.0
68	168049.0
69	140435.0
70	112821.0
71	95562.0
72	78303.0
73	57567.5
74	36832.0
75	36832.0
76	29022.0
77	21212.0
78	18319.5
79	15427.0
80	12244.0
81	9061.0
82	9061.0
83	7575.5
84	6090.0
85	5242.5
86	4395.0
87	2838.0
88	1281.0
89	1281.0
90	915.0
91	549.0
92	417.0
93	285.0
94	295.0
95	305.0
96	305.0
97	156.0
98	7.0
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7590323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.49408891984789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25654730741824	32.47184132534295
2	10.729665902228785	8.904354220546823
3	3.7604108426495344	4.681044656401798
4	1.912886869689283	3.1749399145798627
5	1.140598179573974	2.366404114252955
6	0.7577928111140563	1.8866353372312887
7	0.5178754085849396	1.5042137777258227
8	0.3952934117049434	1.3121871979771973
9	0.3152772235330538	1.1773927032914966
>10	1.9454046194539034	15.249715386168
>50	0.15694101845370437	4.45654802403388
>100	0.08834259015531398	7.146784599111402
>500	0.010591469149958408	3.0731227823947855
>1k	0.011513597320033084	9.308674147622813
>5k	6.679158659196226E-4	1.8248982185482672
>10k+	1.9083310454846356E-4	1.4612435947706506
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31696	0.4175843373200323	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22169	0.29206925713174525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21808	0.28731320129591326	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	12054	0.15880747103911125	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11761	0.15494729275684316	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	11234	0.14800424171672272	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9962	0.1312460615971152	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	8159	0.10749213175776577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.269867962140742E-5
2	0.0	0.0	0.0	0.0	5.269867962140742E-5
3	0.0	0.0	0.0	0.0	5.269867962140742E-5
4	0.0	0.0	0.0	0.0	5.269867962140742E-5
5	0.0	0.0	0.0	0.0	5.269867962140742E-5
6	0.0	0.0	0.0	0.0	6.587334952675927E-5
7	0.0	0.0	0.0	0.0	6.587334952675927E-5
8	0.0	0.0	0.0	0.0	7.904801943211112E-5
9	0.0	0.0	0.0	1.3174669905351855E-5	7.904801943211112E-5
10	0.0	0.0	0.0	3.952400971605556E-5	7.904801943211112E-5
11	0.0	0.0	0.0	3.952400971605556E-5	7.904801943211112E-5
12	0.0	0.0	0.0	3.952400971605556E-5	1.8444537867492596E-4
13	0.0	0.0	0.0	3.952400971605556E-5	1.9762004858027782E-4
14	0.0	0.0	0.0	5.269867962140742E-5	2.1079471848562968E-4
15	0.0	1.3174669905351855E-5	0.0	9.222268933746298E-5	2.634933981070371E-4
16	0.0	1.3174669905351855E-5	0.0	1.712707087695741E-4	2.7666806801238895E-4
17	0.0	1.3174669905351855E-5	0.0	3.5571608744450005E-4	2.7666806801238895E-4
18	0.0	1.3174669905351855E-5	0.0	4.611134466873149E-4	2.7666806801238895E-4
19	0.0	1.3174669905351855E-5	0.0	6.455588253622409E-4	2.7666806801238895E-4
20	0.0	1.3174669905351855E-5	0.0	9.485762331853335E-4	3.820654272552038E-4
21	0.0	1.3174669905351855E-5	0.0	0.0016468337381689818	4.084147670659075E-4
22	0.0	1.3174669905351855E-5	0.0	0.002542711291732908	4.2158943697125935E-4
23	0.0	1.3174669905351855E-5	0.0	0.0038470036123627416	4.874627864980186E-4
24	0.0	1.3174669905351855E-5	0.0	0.005704632069017353	5.006374564033705E-4
25	0.0	1.3174669905351855E-5	0.0	0.00653463627305452	5.006374564033705E-4
26	0.0	1.3174669905351855E-5	0.0	0.008866552846301798	5.006374564033705E-4
27	0.0	1.3174669905351855E-5	0.0	0.021066297178657614	5.006374564033705E-4
28	0.0	2.634933981070371E-5	0.0	0.045953248629867265	5.006374564033705E-4
29	0.0	2.634933981070371E-5	0.0	0.0803391370828356	5.138121263087223E-4
30	0.0	2.634933981070371E-5	0.0	0.13551465464644916	5.138121263087223E-4
31	0.0	2.634933981070371E-5	0.0	0.3389579073248925	5.138121263087223E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	2740	0.0	18.297445	5
CAAGACG	2755	0.0	18.06352	4
CTTATAC	3410	0.0	17.2522	37
GCGCAAG	3225	0.0	15.373644	1
ACGGACC	3210	0.0	15.272586	8
GACGGAC	3225	0.0	15.258915	7
CGCAAGA	3275	0.0	15.025953	2
TCGTTTA	2090	0.0	14.782296	30
TCTTATA	5540	0.0	14.626354	37
AGACGGA	3450	0.0	14.585508	6
CGGACCA	3355	0.0	14.447093	9
CGCATCG	2215	0.0	14.282167	13
GTTACGG	1320	0.0	14.155303	25
ATACCGT	2000	0.0	14.1525	6
TCTAGCG	1715	0.0	14.131195	28
CGAGCCG	2475	0.0	14.0525255	15
GCAAGAC	3935	0.0	13.963152	3
ACCGTCG	1920	0.0	13.874999	8
CTAGCGG	1775	0.0	13.653522	29
TACCGTC	1965	0.0	13.651401	7
>>END_MODULE