FastQCFastQC Report
Thu 9 Feb 2017
SRR2727236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793173
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147380.38854014831382594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97620.25735709918846306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91760.2419082915543267No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT77770.20502624056429802No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG72730.19173921147282236No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC69680.18369844982024283No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT49240.12981216517148045No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC47910.1263058658278966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46830.12345864530829466No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC46750.12324774008462046No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA46320.12211412450737152No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG45430.11976780389399587No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG45400.11968871443511803No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG45380.11963598812919948No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG44720.11789602003388719No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG42960.11325610511305442No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA42750.1127024789009096No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG42110.11101523711151587No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC41880.1104088845934525No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG41600.10967071631059273No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA40950.10795711136823974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA39620.10445081202465588No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC38620.10181449672872817No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG38340.1010763284458684No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA38290.10094451268107202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG17850.019.0700285
CAAGACG18550.018.4501364
CTTATAC20350.018.27272837
GCGCAAG21000.016.2095241
CGCAAGA21100.015.781992
ACGGACC21450.015.438238
CGGACCA21150.015.3947999
TCGTTAC5900.015.36440723
GACGGAC21950.015.1708427
CGTAGAC1101.4535408E-515.1363643
AGACGGA22550.014.8492246
GCAAGAC24950.014.8296593
CGACGGT13650.014.7728947
ACCGTCG12700.014.7125998
CGAGCCG16750.014.68955215
CGTTACA2151.2732926E-1114.62790724
ACGACGG13950.014.4551976
TACCGAA908.2802004E-414.3888892
ACGAACG10950.014.3607315
CGAACGA11100.014.16666716
Produced by FastQC (version 0.11.5)