##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727236.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3793173
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21604287492292	31.0	31.0	34.0	30.0	34.0
2	31.449000348784512	31.0	31.0	34.0	30.0	34.0
3	31.603397999511227	31.0	31.0	34.0	30.0	34.0
4	35.4161958866627	37.0	35.0	37.0	33.0	37.0
5	35.22772306984153	37.0	35.0	37.0	33.0	37.0
6	35.22317252600923	37.0	35.0	37.0	32.0	37.0
7	35.126540761520765	37.0	35.0	37.0	32.0	37.0
8	35.12827492972242	37.0	35.0	37.0	32.0	37.0
9	36.70991199188648	39.0	35.0	39.0	32.0	39.0
10	36.44288304277184	38.0	35.0	39.0	32.0	39.0
11	36.586806612827836	38.0	35.0	39.0	32.0	39.0
12	36.43904061322803	38.0	35.0	39.0	32.0	39.0
13	36.497807244752615	38.0	35.0	39.0	32.0	39.0
14	37.526011336683034	39.0	36.0	41.0	32.0	41.0
15	37.521610008296484	39.0	36.0	41.0	32.0	41.0
16	37.45600978389333	39.0	36.0	41.0	32.0	41.0
17	37.42248402590654	39.0	36.0	41.0	32.0	41.0
18	37.34096177527363	39.0	36.0	41.0	32.0	41.0
19	37.32766525544709	39.0	36.0	41.0	31.0	41.0
20	37.2507971558376	39.0	36.0	40.0	31.0	41.0
21	37.13611823135934	39.0	36.0	40.0	31.0	41.0
22	37.071760502355154	39.0	36.0	40.0	31.0	41.0
23	36.94968724073487	39.0	36.0	40.0	31.0	41.0
24	36.797393369614305	39.0	36.0	40.0	30.0	41.0
25	36.68091489631504	39.0	35.0	40.0	30.0	41.0
26	36.45068890873156	38.0	35.0	40.0	30.0	41.0
27	36.28160329096511	38.0	35.0	40.0	30.0	41.0
28	36.027179619806425	38.0	34.0	40.0	29.0	41.0
29	35.97469242768521	38.0	34.0	40.0	29.0	41.0
30	35.88058968046013	38.0	34.0	40.0	28.0	41.0
31	36.05706726268483	38.0	35.0	40.0	29.0	41.0
32	36.027521022637245	38.0	35.0	40.0	29.0	41.0
33	36.02589889783567	38.0	35.0	40.0	29.0	41.0
34	36.07639909911834	38.0	35.0	40.0	29.0	41.0
35	35.85932410675706	38.0	34.0	40.0	28.0	41.0
36	35.831787266228034	38.0	34.0	40.0	27.0	41.0
37	35.757835985861966	38.0	34.0	40.0	27.0	41.0
38	35.64849059085889	38.0	34.0	40.0	27.0	41.0
39	35.59337420149305	38.0	34.0	40.0	27.0	41.0
40	35.395125663923054	38.0	34.0	40.0	26.0	41.0
41	35.32424542724521	38.0	34.0	40.0	26.0	41.0
42	35.115629316142446	38.0	33.0	40.0	25.0	41.0
43	34.74665827263876	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	5.0
14	10.0
15	27.0
16	101.0
17	223.0
18	359.0
19	674.0
20	1515.0
21	2935.0
22	5286.0
23	9132.0
24	14738.0
25	23046.0
26	33945.0
27	48099.0
28	66166.0
29	87928.0
30	113911.0
31	141745.0
32	175154.0
33	212853.0
34	261947.0
35	316872.0
36	390554.0
37	496768.0
38	675186.0
39	713989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.26045055155671	19.23392368341755	12.416280512383695	26.089345252642048
2	18.358535189404755	22.35091834725176	33.58025062394992	25.710295839393567
3	19.985062637533275	24.06054245350792	29.740483758584173	26.213911150374635
4	14.650636815141308	15.619772681077293	34.408501800471534	35.32108870330987
5	14.571362814192762	37.28092549430252	33.34648854666001	14.8012231448447
6	34.804977257826096	35.12125600387855	15.625098037975068	14.448668700320285
7	28.92261966432852	31.28786902152894	21.25861383069003	18.530897483452506
8	27.997167542846057	33.025464433074895	20.231136307255166	18.746231716823882
9	28.07836605396063	13.322566621664764	18.89555261518523	39.70351470918938
10	17.267627919949867	25.961483960789554	31.946868756051995	24.824019363208585
11	37.551675075194304	21.473262622084466	20.483510770534323	20.491551532186904
12	22.138088613411515	25.338601745820714	28.528411438128447	23.99489820263932
13	30.33726645212333	18.471791294517807	24.856551493960332	26.33439075939853
14	23.28773298765967	20.60551944242986	24.146301790084447	31.960445779826017
15	26.27322824453301	27.115346439511196	21.509670136321226	25.101755179634573
16	25.38370910053404	25.374112912856862	24.35665338754652	24.885524599062578
17	24.70904965315318	25.753979583847087	24.672193965316108	24.86477679768363
18	25.20789323344862	23.68713475499272	26.417540143832092	24.68743186772657
19	25.194184393909797	25.384579084581695	25.734075403362834	23.68716111814568
20	24.8986534492363	23.901757183234196	25.406091417396464	25.793497950133045
21	25.993647007399872	25.13673908361153	25.208525949119643	23.661087959868954
22	25.76592209213764	24.356943382229073	24.514410494854836	25.362724030778455
23	23.77197138121567	24.329499339998463	25.64781516688008	26.250714111905783
24	24.24542724521133	25.62519558164102	25.318618475877585	24.810758697270067
25	25.11330224063073	24.018493224537874	24.56700498500859	26.3011995498228
26	24.42221854895624	25.51528759695379	25.60663592195769	24.45585793213228
27	26.164902049023336	24.435927388495067	24.38987096027521	25.009299602206386
28	24.120993163243543	25.45515324505368	25.721552905707174	24.702300685995603
29	24.322855825452727	25.254318745809908	25.938495291409065	24.4843301373283
30	24.390398223334394	25.896736057121572	25.89172705805931	23.821138661484724
31	25.188305410799877	25.34643160224962	24.391848196747155	25.073414790203348
32	23.982692062819176	25.323601111786886	24.630540183640452	26.063166641753487
33	23.6652006117306	24.71983218271352	25.621979276979985	25.99298792857589
34	25.044415321948144	24.955518770169462	25.805835905717984	24.194230002164417
35	25.264125838710754	24.82357118960828	26.183488071859628	23.728814899821337
36	23.909007050297994	25.538750803087545	25.734998113716408	24.81724403289805
37	24.427649358465857	25.163998583771424	26.012892109060147	24.395459948702577
38	24.011375173238868	24.34803263652884	26.61771556425188	25.022876625980413
39	24.44278180826448	23.81768508844706	26.155938577017185	25.583594526271273
40	24.524744850814873	24.28341654862565	26.955559369425018	24.23627923113446
41	23.033855824661835	24.631146536158514	27.575647090180173	24.75935054899948
42	24.519366767611178	24.593552680038584	27.229525254977826	23.657555297372408
43	23.396322814698934	23.257968987968646	27.380111584681217	25.965596612651204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	346.0
1	418.0
2	490.0
3	1115.5
4	1741.0
5	1741.0
6	2673.5
7	3606.0
8	3515.5
9	3425.0
10	4413.0
11	5401.0
12	5401.0
13	9574.0
14	13747.0
15	22076.0
16	30405.0
17	27673.5
18	24942.0
19	24942.0
20	26822.0
21	28702.0
22	24771.0
23	20840.0
24	24663.0
25	28486.0
26	28486.0
27	33859.5
28	39233.0
29	44837.5
30	50442.0
31	57000.5
32	63559.0
33	63559.0
34	76162.5
35	88766.0
36	109727.0
37	130688.0
38	151681.5
39	172675.0
40	172675.0
41	190487.5
42	208300.0
43	219295.5
44	230291.0
45	255151.0
46	280011.0
47	280011.0
48	324134.0
49	368257.0
50	369949.5
51	371642.0
52	341613.0
53	311584.0
54	311584.0
55	301114.5
56	290645.0
57	281767.0
58	272889.0
59	255904.0
60	238919.0
61	238919.0
62	198100.5
63	157282.0
64	139638.5
65	121995.0
66	103885.0
67	85775.0
68	85775.0
69	71265.5
70	56756.0
71	48874.5
72	40993.0
73	29694.0
74	18395.0
75	18395.0
76	14500.5
77	10606.0
78	9489.5
79	8373.0
80	6753.0
81	5133.0
82	5133.0
83	4311.0
84	3489.0
85	3156.0
86	2823.0
87	1819.0
88	815.0
89	815.0
90	585.0
91	355.0
92	259.5
93	164.0
94	169.5
95	175.0
96	175.0
97	91.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3793173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.235701868919016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72700911904342	34.902888990404676
2	9.640533474751786	8.336304623434042
3	3.3026041323580246	4.283712229730745
4	1.6867348829537356	2.917086661251749
5	0.9968371945826933	2.154947787841347
6	0.6820968605325511	1.7694561904626607
7	0.4798504321351359	1.4522669157826686
8	0.3496630160491325	1.209434073318987
9	0.27994465490696563	1.0893243275419484
>10	1.6260379566156349	12.83273728619375
>50	0.11200824699527094	3.3554917132638784
>100	0.0859263139703285	7.892328192149263
>500	0.01638567723443711	5.0459084568887675
>1k	0.01400119435068669	11.28586606797712
>5k	3.057029334211068E-4	1.0826539122493088
>10k+	6.114058668422135E-5	0.38959257150918825
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14738	0.38854014831382594	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9762	0.25735709918846306	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9176	0.2419082915543267	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7777	0.20502624056429802	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	7273	0.19173921147282236	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	6968	0.18369844982024283	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	4924	0.12981216517148045	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	4791	0.1263058658278966	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4683	0.12345864530829466	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	4675	0.12324774008462046	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	4632	0.12211412450737152	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	4543	0.11976780389399587	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	4540	0.11968871443511803	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	4538	0.11963598812919948	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	4472	0.11789602003388719	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	4296	0.11325610511305442	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	4275	0.1127024789009096	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	4211	0.11101523711151587	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	4188	0.1104088845934525	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4160	0.10967071631059273	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA	4095	0.10795711136823974	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3962	0.10445081202465588	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	3862	0.10181449672872817	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	3834	0.1010763284458684	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	3829	0.10094451268107202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6363152959277102E-5
2	0.0	0.0	0.0	0.0	2.6363152959277102E-5
3	0.0	0.0	0.0	0.0	2.6363152959277102E-5
4	0.0	0.0	0.0	0.0	2.6363152959277102E-5
5	0.0	0.0	0.0	0.0	2.6363152959277102E-5
6	0.0	0.0	0.0	0.0	7.908945887783131E-5
7	0.0	0.0	0.0	0.0	7.908945887783131E-5
8	0.0	0.0	0.0	0.0	7.908945887783131E-5
9	0.0	0.0	0.0	2.6363152959277102E-5	1.0545261183710841E-4
10	0.0	0.0	0.0	2.6363152959277102E-5	1.0545261183710841E-4
11	0.0	0.0	0.0	2.6363152959277102E-5	1.0545261183710841E-4
12	0.0	0.0	0.0	5.2726305918554204E-5	1.0545261183710841E-4
13	0.0	0.0	0.0	7.908945887783131E-5	1.318157647963855E-4
14	0.0	0.0	0.0	7.908945887783131E-5	2.1090522367421682E-4
15	0.0	0.0	0.0	7.908945887783131E-5	2.63631529592771E-4
16	0.0	0.0	0.0	2.899946825520481E-4	3.427209884706023E-4
17	0.0	0.0	0.0	6.854419769412046E-4	3.427209884706023E-4
18	0.0	0.0	0.0	8.963472006154214E-4	3.954472943891565E-4
19	0.0	0.0	0.0	0.0012390681890860238	3.954472943891565E-4
20	0.0	0.0	0.0	0.0016081523305159031	3.954472943891565E-4
21	0.0	0.0	0.0	0.002319957460416385	4.2181044734843363E-4
22	0.0	0.0	0.0	0.0034535730376653003	4.481736003077107E-4
23	0.0	0.0	0.0	0.005298993744814697	5.799893651040962E-4
24	0.0	0.0	0.0	0.007777130122986745	5.799893651040962E-4
25	0.0	0.0	0.0	0.009174377229828432	5.799893651040962E-4
26	0.0	0.0	0.0	0.012179776667186022	5.799893651040962E-4
27	0.0	0.0	0.0	0.02657405818295132	5.799893651040962E-4
28	0.0	0.0	0.0	0.05536262121448191	5.799893651040962E-4
29	0.0	0.0	0.0	0.0972800344197325	5.799893651040962E-4
30	0.0	0.0	0.0	0.16368881672415153	5.799893651040962E-4
31	0.0	0.0	0.0	0.41624782207402616	5.799893651040962E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1785	0.0	19.070028	5
CAAGACG	1855	0.0	18.450136	4
CTTATAC	2035	0.0	18.272728	37
GCGCAAG	2100	0.0	16.209524	1
CGCAAGA	2110	0.0	15.78199	2
ACGGACC	2145	0.0	15.43823	8
CGGACCA	2115	0.0	15.394799	9
TCGTTAC	590	0.0	15.364407	23
GACGGAC	2195	0.0	15.170842	7
CGTAGAC	110	1.4535408E-5	15.136364	3
AGACGGA	2255	0.0	14.849224	6
GCAAGAC	2495	0.0	14.829659	3
CGACGGT	1365	0.0	14.772894	7
ACCGTCG	1270	0.0	14.712599	8
CGAGCCG	1675	0.0	14.689552	15
CGTTACA	215	1.2732926E-11	14.627907	24
ACGACGG	1395	0.0	14.455197	6
TACCGAA	90	8.2802004E-4	14.388889	2
ACGAACG	1095	0.0	14.36073	15
CGAACGA	1110	0.0	14.166667	16
>>END_MODULE