FastQCFastQC Report
Thu 9 Feb 2017
SRR2727235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314180
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114340.3450023836967213No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG101590.30653132901652896No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC92080.2778364482315384No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT71260.21501547894200074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67080.2024030076821416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63420.1913595519857099No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA62470.18849308124483283No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG62000.18707493256250415No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG46340.13982342540236198No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG44140.13318528263401505No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG41680.12576263208395438No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG40630.12259442758087974No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT39810.12012021073085952No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC39400.11888310230584942No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC39210.118309808157674No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG39050.11782703413815786No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG38760.11695200622778486No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT37380.11278808030945814No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA37360.11272773355701864No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG36830.11112854461737141No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA35930.10841294075759313No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA35610.10744739271856085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35430.10690427194660519No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA34280.10343433368133295No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33530.10117133046485105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG16050.018.9034274
AAGACGG15950.018.789975
CTTATAC13550.017.61254537
CGCAAGA17850.016.8935592
AGACGGA17850.016.7899176
GCGCAAG17900.016.7430171
ACGGACC17550.016.6552728
GACGGAC17750.016.5718337
CGGACCA18150.016.1046859
TAGTACT1659.767973E-1015.696974
CGTTATA957.066094E-515.5789462
AGTACTA1557.223207E-915.5161295
CGTTACG2900.015.31034624
TCTTATA22600.014.98008837
TCGTTAC3250.014.823
GCAAGAC21650.014.782913
GCGGGTA7650.014.75163423
TAAGACT4200.014.5357144
TCTAGAC2950.014.4237293
TTACACT3350.014.3582094
Produced by FastQC (version 0.11.5)