##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727235.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3314180
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.973770887519688	31.0	31.0	33.0	28.0	34.0
2	31.21050636959972	31.0	31.0	34.0	28.0	34.0
3	31.364436150118582	31.0	31.0	34.0	28.0	34.0
4	35.2031009178741	35.0	35.0	37.0	33.0	37.0
5	34.98982010633098	35.0	35.0	37.0	32.0	37.0
6	35.00789003614771	36.0	35.0	37.0	32.0	37.0
7	34.91376931850412	36.0	35.0	37.0	32.0	37.0
8	34.92229721982512	36.0	35.0	37.0	32.0	37.0
9	36.47470324484488	38.0	35.0	39.0	32.0	39.0
10	36.19097665184148	38.0	35.0	39.0	32.0	39.0
11	36.340384046732524	38.0	35.0	39.0	32.0	39.0
12	36.17529705688889	38.0	35.0	39.0	31.0	39.0
13	36.245724734323424	38.0	35.0	39.0	32.0	39.0
14	37.2133321062827	39.0	36.0	40.0	31.0	41.0
15	37.213225895998406	39.0	36.0	40.0	31.0	41.0
16	37.13557501403062	39.0	36.0	40.0	31.0	41.0
17	37.10239968861076	39.0	36.0	40.0	31.0	41.0
18	37.00971944794791	39.0	36.0	40.0	31.0	41.0
19	36.99195970043872	39.0	36.0	40.0	31.0	41.0
20	36.911638474675485	39.0	36.0	40.0	31.0	41.0
21	36.78338140957944	39.0	36.0	40.0	30.0	41.0
22	36.71662734069966	39.0	35.0	40.0	30.0	41.0
23	36.586120548672675	38.0	35.0	40.0	30.0	41.0
24	36.42873531310912	38.0	35.0	40.0	30.0	41.0
25	36.30895213899064	38.0	35.0	40.0	30.0	41.0
26	36.04243432764666	38.0	34.0	40.0	29.0	41.0
27	35.86157903312433	38.0	34.0	40.0	27.0	41.0
28	35.610153039364185	38.0	34.0	40.0	27.0	41.0
29	35.55818875257228	38.0	34.0	40.0	27.0	41.0
30	35.45341683312313	38.0	34.0	40.0	27.0	41.0
31	35.652657067509914	38.0	34.0	40.0	27.0	41.0
32	35.60428824022835	38.0	34.0	40.0	27.0	41.0
33	35.618011091733095	38.0	34.0	40.0	27.0	41.0
34	35.677517515644894	38.0	34.0	40.0	27.0	41.0
35	35.44661213331805	38.0	34.0	40.0	26.0	41.0
36	35.414273515620756	38.0	34.0	40.0	26.0	41.0
37	35.32762976060443	38.0	34.0	40.0	26.0	41.0
38	35.228677983694304	38.0	33.0	40.0	26.0	41.0
39	35.175724311896154	38.0	33.0	40.0	26.0	41.0
40	34.954978607076264	38.0	33.0	40.0	25.0	41.0
41	34.88447247886355	38.0	33.0	40.0	25.0	41.0
42	34.663345684302	38.0	33.0	40.0	24.0	41.0
43	34.28862855970406	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	10.0
13	6.0
14	11.0
15	52.0
16	106.0
17	212.0
18	439.0
19	847.0
20	1783.0
21	3352.0
22	6012.0
23	10359.0
24	16596.0
25	24930.0
26	36098.0
27	49780.0
28	67785.0
29	88488.0
30	112000.0
31	136778.0
32	165254.0
33	198658.0
34	239779.0
35	286469.0
36	349712.0
37	440865.0
38	595815.0
39	481977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.9497070165169	20.453023070563457	12.967461031084612	25.629808881835025
2	18.972747406598316	23.23775413526121	32.50843345865342	25.28106499948705
3	20.865402603358902	24.485091334809816	29.445594385338154	25.203911676493128
4	15.531262635101292	16.466576951161375	33.8566704282809	34.145489985456436
5	14.592206820389961	37.456776638565195	33.36526682316591	14.585749717878931
6	34.808821488271604	35.35830884260964	15.32535951577766	14.507510153341096
7	28.709786432843114	31.21695260969531	21.315378162924162	18.757882794537412
8	27.91553265061041	33.18636887555896	20.18260927288198	18.71548920094865
9	27.81635276297606	13.340675521546807	18.825531504022113	40.01744021145502
10	17.338889257674598	26.076042942749034	32.12420568587102	24.46086211370535
11	37.53948186278355	21.423338503038458	20.345334290835137	20.691845343342848
12	22.004206168645034	25.63059338961674	28.515077636097015	23.850122805641213
13	30.683064890862898	18.477300569069875	24.661545238942967	26.178089301124256
14	23.26279803752361	20.585152285029782	24.06906082349178	32.082988853954824
15	26.40601898508832	27.074479961860852	21.426325667284214	25.093175385766614
16	25.412409706171662	25.394396200568465	24.339293580915943	24.85390051234393
17	24.83464989831572	26.0659046883392	24.602284728047362	24.497160685297718
18	25.48874834800765	23.426247216506045	26.43721825609955	24.647786179386756
19	25.252279598573402	25.723346348116277	25.493214007688174	23.531160045622144
20	25.111128544617372	23.757822447784974	25.32626471706425	25.804784290533405
21	25.906076314503135	25.301673415445148	24.88413423531613	23.908116034735592
22	25.734872577832224	24.732120765920982	24.273666487637968	25.259340168608823
23	23.774870405349134	24.212686094297837	25.485761183761895	26.52668231659113
24	24.402899057987195	25.96953092469329	24.999577572732925	24.627992444586592
25	25.302940697246378	24.11217857810982	24.21473788388078	26.370142840763023
26	24.57690891864654	25.590764533006656	25.355442371868758	24.47688417647804
27	26.243505180768693	24.356190671599006	24.14455461079362	25.255749536838675
28	24.47359527847009	25.071028127621314	25.875208950630324	24.580167643278276
29	24.63091926207991	24.798472020228232	25.79911169580409	24.771497021887768
30	24.326379375893886	25.903451230772017	25.856320417116752	23.913848976217345
31	25.618946466395908	25.22418818531281	23.928694277317465	25.228171070973815
32	23.975493183834313	25.340838457778396	24.535299832839495	26.1483685255478
33	23.96327296646531	24.722827366045298	25.502296193930324	25.81160347355907
34	25.11740460687108	24.825477191944916	25.508873989946228	24.548244211237773
35	25.209433404341347	25.024742168500204	26.109625910481626	23.656198516676824
36	24.30589165344067	25.53397823896107	25.26760767369304	24.89252243390522
37	24.599146696920503	25.201045205752255	25.6852072005745	24.514600896752743
38	24.334224453711023	24.316391988365147	26.375121447839284	24.974262110084545
39	25.025526676281917	23.50315915249021	25.715984044318656	25.75533012690922
40	24.713835699931806	24.32864237911037	26.637629820951187	24.319892100006637
41	23.43044131580059	24.515415577910673	27.10054975891472	24.95359334737401
42	24.999185318842066	24.638221219125093	26.726943014561673	23.635650447471168
43	24.017796257294414	23.143522681326907	27.06820389960714	25.770477161771538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169.0
1	209.0
2	249.0
3	560.5
4	872.0
5	872.0
6	1529.0
7	2186.0
8	2195.0
9	2204.0
10	2953.5
11	3703.0
12	3703.0
13	6881.0
14	10059.0
15	16758.0
16	23457.0
17	20957.5
18	18458.0
19	18458.0
20	20552.5
21	22647.0
22	20162.5
23	17678.0
24	20791.5
25	23905.0
26	23905.0
27	28668.0
28	33431.0
29	38351.0
30	43271.0
31	49813.5
32	56356.0
33	56356.0
34	67614.5
35	78873.0
36	97589.0
37	116305.0
38	133565.5
39	150826.0
40	150826.0
41	166391.5
42	181957.0
43	191626.0
44	201295.0
45	223477.5
46	245660.0
47	245660.0
48	293523.0
49	341386.0
50	341153.0
51	340920.0
52	303671.5
53	266423.0
54	266423.0
55	256310.5
56	246198.0
57	239968.0
58	233738.0
59	220286.0
60	206834.0
61	206834.0
62	171683.0
63	136532.0
64	121033.0
65	105534.0
66	89383.0
67	73232.0
68	73232.0
69	61177.5
70	49123.0
71	42708.5
72	36294.0
73	26289.0
74	16284.0
75	16284.0
76	12764.0
77	9244.0
78	8325.0
79	7406.0
80	5983.0
81	4560.0
82	4560.0
83	3799.5
84	3039.0
85	2751.5
86	2464.0
87	1625.5
88	787.0
89	787.0
90	544.0
91	301.0
92	229.0
93	157.0
94	147.0
95	137.0
96	137.0
97	75.0
98	13.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3314180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.555485080949246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.20705572525806	37.44982300640757
2	9.160307783943853	8.346045291767151
3	2.99945416071593	4.099247678084571
4	1.5130077763358234	2.7570321272890723
5	0.915849156125168	2.0860976284130026
6	0.6040118253757775	1.6509631019773892
7	0.43366333822349	1.3829020614216394
8	0.3015495567448742	1.098978906676638
9	0.24687875944198537	1.012201347830636
>10	1.4184971671022593	11.543753137911034
>50	0.0886610354202967	2.808464975964663
>100	0.08116042896387238	7.985864152063302
>500	0.01648003336560607	5.31962028259688
>1k	0.012891639003592218	10.539008596087324
>5k	3.9871048464718196E-4	1.266325429161711
>10k+	1.3290349488239401E-4	0.6536722763474175
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11434	0.3450023836967213	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	10159	0.30653132901652896	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	9208	0.2778364482315384	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7126	0.21501547894200074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6708	0.2024030076821416	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6342	0.1913595519857099	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6247	0.18849308124483283	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	6200	0.18707493256250415	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	4634	0.13982342540236198	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	4414	0.13318528263401505	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	4168	0.12576263208395438	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	4063	0.12259442758087974	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	3981	0.12012021073085952	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	3940	0.11888310230584942	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	3921	0.118309808157674	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	3905	0.11782703413815786	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	3876	0.11695200622778486	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3738	0.11278808030945814	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	3736	0.11272773355701864	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	3683	0.11112854461737141	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	3593	0.10841294075759313	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	3561	0.10744739271856085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3543	0.10690427194660519	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA	3428	0.10343433368133295	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	3353	0.10117133046485105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0173376219758733E-5
2	0.0	0.0	0.0	0.0	3.0173376219758733E-5
3	0.0	0.0	0.0	0.0	3.0173376219758733E-5
4	0.0	0.0	0.0	0.0	3.0173376219758733E-5
5	0.0	0.0	0.0	0.0	3.0173376219758733E-5
6	0.0	0.0	0.0	0.0	3.0173376219758733E-5
7	0.0	0.0	0.0	0.0	3.0173376219758733E-5
8	0.0	0.0	0.0	0.0	3.0173376219758733E-5
9	0.0	0.0	0.0	0.0	6.0346752439517466E-5
10	0.0	0.0	0.0	0.0	9.05201286592762E-5
11	0.0	0.0	0.0	3.0173376219758733E-5	9.05201286592762E-5
12	0.0	0.0	0.0	3.0173376219758733E-5	9.05201286592762E-5
13	0.0	0.0	0.0	3.0173376219758733E-5	9.05201286592762E-5
14	0.0	0.0	0.0	6.0346752439517466E-5	1.2069350487903493E-4
15	0.0	0.0	0.0	6.0346752439517466E-5	1.5086688109879366E-4
16	0.0	0.0	0.0	1.810402573185524E-4	2.1121363353831114E-4
17	0.0	0.0	0.0	4.224272670766223E-4	2.1121363353831114E-4
18	0.0	0.0	0.0	4.8277401951613973E-4	2.4138700975806987E-4
19	0.0	0.0	0.0	8.146811579334858E-4	2.4138700975806987E-4
20	0.0	0.0	0.0	0.0010862415439113144	2.4138700975806987E-4
21	0.0	0.0	0.0	0.0018405759494052828	2.4138700975806987E-4
22	0.0	0.0	0.0	0.003288898007953702	2.4138700975806987E-4
23	0.0	0.0	0.0	0.004918260323820673	2.715603859778286E-4
24	0.0	0.0	0.0	0.007603690807379201	2.715603859778286E-4
25	0.0	0.0	0.0	0.00905201286592762	2.715603859778286E-4
26	0.0	0.0	0.0	0.012009003735463975	2.715603859778286E-4
27	0.0	0.0	0.0	0.023716273708730366	2.715603859778286E-4
28	0.0	0.0	0.0	0.04658769288330748	2.715603859778286E-4
29	0.0	0.0	0.0	0.0759765613213525	2.715603859778286E-4
30	0.0	0.0	0.0	0.12473673729248261	2.715603859778286E-4
31	0.0	0.0	0.0	0.32517847552033985	3.017337621975873E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	1605	0.0	18.903427	4
AAGACGG	1595	0.0	18.78997	5
CTTATAC	1355	0.0	17.612545	37
CGCAAGA	1785	0.0	16.893559	2
AGACGGA	1785	0.0	16.789917	6
GCGCAAG	1790	0.0	16.743017	1
ACGGACC	1755	0.0	16.655272	8
GACGGAC	1775	0.0	16.571833	7
CGGACCA	1815	0.0	16.104685	9
TAGTACT	165	9.767973E-10	15.69697	4
CGTTATA	95	7.066094E-5	15.578946	2
AGTACTA	155	7.223207E-9	15.516129	5
CGTTACG	290	0.0	15.310346	24
TCTTATA	2260	0.0	14.980088	37
TCGTTAC	325	0.0	14.8	23
GCAAGAC	2165	0.0	14.78291	3
GCGGGTA	765	0.0	14.751634	23
TAAGACT	420	0.0	14.535714	4
TCTAGAC	295	0.0	14.423729	3
TTACACT	335	0.0	14.358209	4
>>END_MODULE