##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6158102
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210270144924525	31.0	31.0	34.0	30.0	34.0
2	31.43418394174049	31.0	31.0	34.0	30.0	34.0
3	31.589729757642857	31.0	31.0	34.0	30.0	34.0
4	35.404165439286324	37.0	35.0	37.0	33.0	37.0
5	35.22459079112363	37.0	35.0	37.0	33.0	37.0
6	35.240933976085486	37.0	35.0	37.0	32.0	37.0
7	35.13297149024164	37.0	35.0	37.0	32.0	37.0
8	35.14895384974137	37.0	35.0	37.0	32.0	37.0
9	36.70503606468357	39.0	35.0	39.0	32.0	39.0
10	36.44655398692649	38.0	35.0	39.0	32.0	39.0
11	36.60424413236416	39.0	35.0	39.0	32.0	39.0
12	36.46566214720055	38.0	35.0	39.0	32.0	39.0
13	36.530577440906306	38.0	35.0	39.0	32.0	39.0
14	37.53515579962787	39.0	36.0	41.0	32.0	41.0
15	37.53384013450898	39.0	36.0	41.0	32.0	41.0
16	37.4959545976991	39.0	36.0	41.0	32.0	41.0
17	37.48534451037024	39.0	36.0	41.0	32.0	41.0
18	37.43547817168342	39.0	36.0	41.0	32.0	41.0
19	37.45388887680003	39.0	36.0	41.0	32.0	41.0
20	37.381461365855905	39.0	36.0	41.0	32.0	41.0
21	37.275442660741895	39.0	36.0	41.0	31.0	41.0
22	37.201043275996405	39.0	36.0	40.0	31.0	41.0
23	37.08659518793291	39.0	36.0	40.0	31.0	41.0
24	36.949678163823855	39.0	36.0	40.0	31.0	41.0
25	36.822972240472794	39.0	35.0	40.0	30.0	41.0
26	36.58420597775093	39.0	35.0	40.0	30.0	41.0
27	36.40372796683134	38.0	35.0	40.0	30.0	41.0
28	36.18627508930511	38.0	35.0	40.0	29.0	41.0
29	36.10521082632279	38.0	35.0	40.0	29.0	41.0
30	35.9889899517741	38.0	35.0	40.0	29.0	41.0
31	36.09612458514003	38.0	35.0	40.0	30.0	41.0
32	36.047896251150114	38.0	35.0	40.0	30.0	41.0
33	35.99895227458071	38.0	35.0	40.0	29.0	41.0
34	36.026420315870055	38.0	35.0	40.0	29.0	41.0
35	35.79184787780391	38.0	34.0	40.0	28.0	41.0
36	35.74471452405303	38.0	34.0	40.0	27.0	41.0
37	35.62957174142293	38.0	34.0	40.0	27.0	41.0
38	35.51348662298871	38.0	34.0	40.0	27.0	41.0
39	35.420423695482796	38.0	34.0	40.0	26.0	41.0
40	35.19079986008676	38.0	34.0	40.0	25.0	41.0
41	35.08850876455116	38.0	33.0	40.0	25.0	41.0
42	34.83881088686092	38.0	33.0	40.0	24.0	41.0
43	34.460656384061195	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	9.0
14	6.0
15	48.0
16	119.0
17	294.0
18	510.0
19	1099.0
20	2194.0
21	4537.0
22	8153.0
23	14608.0
24	23225.0
25	36445.0
26	54637.0
27	78124.0
28	107965.0
29	143228.0
30	184452.0
31	229547.0
32	283474.0
33	344450.0
34	420903.0
35	513187.0
36	639278.0
37	829079.0
38	1085114.0
39	1153411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60817699999123	19.471307880252713	13.151893229439851	24.768621890316204
2	17.89850184358752	23.03781262473405	34.32153283592899	24.742152695749436
3	20.084402629251674	24.902413113650926	30.137662546024735	24.875521711072665
4	14.497096670370189	15.918671045072005	34.24345683134187	35.34077545321595
5	14.145007666323162	38.2502108604242	33.02962503706499	14.575156436187644
6	33.80947571183459	35.36955055307626	15.993905264966383	14.827068470122775
7	27.97777626937651	31.976881837942923	21.559337601098523	18.486004291582052
8	28.131800999723616	33.22754316183785	20.04807650149348	18.592579336945054
9	27.66595941411818	13.578323321049247	19.87584811034309	38.879869154489484
10	17.16171638599036	27.279736516218794	32.04363942006807	23.51490767772278
11	35.64642157599857	22.7448002647569	20.928396444229083	20.68038171501544
12	21.47120979808389	26.4900126694881	29.375106160956737	22.663671371471274
13	30.07439629937926	19.5502445396325	25.494754715007968	24.88060444598027
14	22.813262917697692	21.234627162719942	25.92988878716202	30.022221132420345
15	25.270838319988854	27.972628579390207	23.000690797261882	23.755842303359053
16	23.882520945577063	26.330401802373522	25.434784938606082	24.35229231344333
17	23.346755217760276	26.760501855928986	26.19018002624835	23.70256290006239
18	23.23147619185262	24.809056426801636	28.0071034874057	23.95236389394005
19	23.451170506756792	25.993219988886185	28.233358265257703	22.32225123909932
20	23.530691761844803	25.14123669922973	27.679340160328618	23.648731378596846
21	24.413220177255916	25.747040240645575	27.52620856231352	22.313531019784993
22	24.399384745494636	25.34688447836687	26.890947892711097	23.362782883427393
23	22.914089438596502	25.676628935344038	27.429393017523907	23.979888608535553
24	22.929370120858668	26.497726085082707	27.366321636114506	23.20658215794412
25	23.494576738092356	25.55236986980729	26.907641347934803	24.045412044165555
26	23.13160775836451	26.539313574214912	27.482298929118095	22.846779738302484
27	23.892020625835688	26.125322380174932	26.771560458076205	23.211096535913175
28	22.81931997878567	26.64393996721717	27.72146352886003	22.81527652513713
29	22.86506459295413	26.69622880556379	27.754282082368885	22.684424519113193
30	22.860810035299835	27.128001452395562	27.59376184415263	22.417426668151975
31	23.151711355219515	26.709106149914376	26.94063852791006	23.198543966956052
32	22.390226728949926	26.844586205295073	27.00908494208118	23.75610212367382
33	22.17680057913948	26.49590734287935	27.612127892652637	23.715164185328533
34	23.131981250066985	26.465703231287822	27.67066865732331	22.73164686132188
35	23.036887014862696	26.3410057189699	27.92210976693793	22.69999749922947
36	22.137177981137697	26.780589214014316	27.684114358612444	23.398118446235543
37	22.444545413505654	26.27190975401187	27.95432748596889	23.329217346513584
38	22.0274850919975	25.792833571123055	28.457242182737474	23.722439154141973
39	22.299500722787638	25.39461022243542	28.54580843253327	23.76008062224367
40	22.24104115196533	25.679194660952355	29.015449890242156	23.064314296840163
41	21.231346931246023	25.898694110620447	29.562063116200417	23.307895841933117
42	21.819385258639755	25.948108686734972	29.281538370101696	22.950967684523576
43	21.07605557686443	25.20667894101137	29.31393471559906	24.40333076652514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3075.0
1	2835.0
2	2595.0
3	5102.0
4	7609.0
5	7609.0
6	11486.0
7	15363.0
8	14597.0
9	13831.0
10	18659.5
11	23488.0
12	23488.0
13	41487.5
14	59487.0
15	90050.0
16	120613.0
17	104548.5
18	88484.0
19	88484.0
20	90336.0
21	92188.0
22	73788.0
23	55388.0
24	64692.5
25	73997.0
26	73997.0
27	83207.0
28	92417.0
29	101800.5
30	111184.0
31	121767.0
32	132350.0
33	132350.0
34	152060.5
35	171771.0
36	201096.0
37	230421.0
38	260511.5
39	290602.0
40	290602.0
41	319347.5
42	348093.0
43	369183.5
44	390274.0
45	422471.0
46	454668.0
47	454668.0
48	490224.5
49	525781.0
50	523312.0
51	520843.0
52	497988.5
53	475134.0
54	475134.0
55	454092.5
56	433051.0
57	411425.0
58	389799.0
59	357795.5
60	325792.0
61	325792.0
62	276078.5
63	226365.0
64	199565.0
65	172765.0
66	145033.5
67	117302.0
68	117302.0
69	96424.5
70	75547.0
71	63694.5
72	51842.0
73	38537.0
74	25232.0
75	25232.0
76	19961.5
77	14691.0
78	12730.5
79	10770.0
80	8654.5
81	6539.0
82	6539.0
83	5375.0
84	4211.0
85	3538.5
86	2866.0
87	1886.5
88	907.0
89	907.0
90	662.0
91	417.0
92	296.0
93	175.0
94	166.5
95	158.0
96	158.0
97	84.5
98	11.0
99	8.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6158102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.75860308513499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.35416514988879	36.76894349331148
2	9.97011322574575	9.124369076215096
3	3.3896640263030178	4.653188723146811
4	1.6691323343021802	3.055086559275933
5	0.9838352138372924	2.250946252557979
6	0.6446566739138632	1.7699153320684662
7	0.46490744357139585	1.4891460627195812
8	0.34188716672957314	1.2515423329823927
9	0.2683460667715425	1.105122704297053
>10	1.6922453012161887	14.484841895709883
>50	0.12578585819970933	3.929820963849423
>100	0.07622972942986701	6.840837908145936
>500	0.010190554239177857	3.2855507598634683
>1k	0.008486185335812527	6.938605519811871
>5k	2.1304230967873284E-4	0.6115895549338103
>10k+	1.4202820645248856E-4	2.4404928611110686
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	56824	0.9227518478907949	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	37285	0.6054625272527152	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35025	0.5687629077920437	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21073	0.3421995933162523	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	8882	0.1442327522343735	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6626	0.10759808785239348	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6247	0.10144359414637821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6238769672863488E-5
2	0.0	0.0	0.0	0.0	1.6238769672863488E-5
3	1.6238769672863488E-5	0.0	0.0	0.0	1.6238769672863488E-5
4	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	3.2477539345726976E-5
5	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	3.2477539345726976E-5
6	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	6.495507869145395E-5
7	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	6.495507869145395E-5
8	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	6.495507869145395E-5
9	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	1.299101573829079E-4
10	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	1.299101573829079E-4
11	1.6238769672863488E-5	1.6238769672863488E-5	0.0	0.0	1.299101573829079E-4
12	1.6238769672863488E-5	1.6238769672863488E-5	0.0	8.119384836431744E-5	1.461489270557714E-4
13	1.6238769672863488E-5	3.2477539345726976E-5	0.0	1.7862646640149838E-4	1.461489270557714E-4
14	1.6238769672863488E-5	3.2477539345726976E-5	0.0	3.2477539345726977E-4	2.598203147658158E-4
15	1.6238769672863488E-5	3.2477539345726976E-5	0.0	5.521181688773586E-4	3.085366237844063E-4
16	4.871630901859047E-5	3.2477539345726976E-5	0.0	9.093711016803554E-4	4.059692418215872E-4
17	4.871630901859047E-5	3.2477539345726976E-5	0.0	0.0014777280402305775	4.059692418215872E-4
18	4.871630901859047E-5	3.2477539345726976E-5	0.0	0.0016888320459778029	4.059692418215872E-4
19	4.871630901859047E-5	3.2477539345726976E-5	0.0	0.0021759951361637075	4.059692418215872E-4
20	4.871630901859047E-5	4.871630901859047E-5	0.0	0.0028742622320968377	4.059692418215872E-4
21	4.871630901859047E-5	4.871630901859047E-5	0.0	0.003881065951814374	4.059692418215872E-4
22	4.871630901859047E-5	4.871630901859047E-5	0.0	0.0064467915601268055	4.059692418215872E-4
23	4.871630901859047E-5	4.871630901859047E-5	0.0	0.010344096281614043	5.358793992044952E-4
24	4.871630901859047E-5	4.871630901859047E-5	0.0	0.015735367813004723	5.358793992044952E-4
25	8.119384836431744E-5	4.871630901859047E-5	0.0	0.018869450359867375	5.521181688773586E-4
26	8.119384836431744E-5	4.871630901859047E-5	0.0	0.024065856655183692	5.683569385502221E-4
27	8.119384836431744E-5	4.871630901859047E-5	0.0	0.05521181688773586	5.683569385502221E-4
28	8.119384836431744E-5	4.871630901859047E-5	0.0	0.11417478956990319	5.683569385502221E-4
29	8.119384836431744E-5	4.871630901859047E-5	0.0	0.19442678929319457	5.683569385502221E-4
30	8.119384836431744E-5	4.871630901859047E-5	0.0	0.32346005311376785	5.683569385502221E-4
31	8.119384836431744E-5	4.871630901859047E-5	0.0	0.71647075673641	5.683569385502221E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	1990	0.0	17.75628	4
AAGACGG	1925	0.0	17.683117	5
CTTATAC	5205	0.0	17.415945	37
CGCAAGA	2265	0.0	15.110375	2
CTAGCGG	1010	0.0	14.653466	29
GACGGAC	2255	0.0	14.603105	7
GTACTAG	305	0.0	14.557376	1
ACGGACC	2225	0.0	14.550562	8
CGGACCA	2285	0.0	14.330417	9
TCTTATA	8445	0.0	14.173476	37
AGACGGA	2405	0.0	14.153846	6
GCGCAAG	2430	0.0	14.084362	1
TCTAGCG	1025	0.0	14.07805	28
ATACCGT	1600	0.0	13.990625	6
TACCGTC	1545	0.0	13.889967	7
ACCGTCG	1625	0.0	13.889231	8
CGTTATA	230	4.0017767E-11	13.673913	2
CGTCGTA	1540	0.0	13.214285	10
CCGTCGT	1620	0.0	13.132716	9
GCGTTAG	155	1.8911287E-6	13.129032	1
>>END_MODULE