FastQCFastQC Report
Thu 9 Feb 2017
SRR2727233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3953523
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT508121.2852334487493813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT325750.8239486655319825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT308660.7807213970931749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193900.4904486454233351No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA87780.22202981998587085No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53120.13436117609534584No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.1293529846670931No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA48270.12209363648573691No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.12037365155078142No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41910.10600671856468269No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40530.10251616090256715No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT40150.10155499285068026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC34950.017.89127337
TATACCG1705.456968E-1217.4117645
ATACCGA1606.311893E-1016.18756
AAGACGG10450.015.75598055
GGAGTGT16800.015.7470256
GGACCGT1701.4897523E-915.2352936
GAACAGT31100.015.1688111
CAAGACG11250.014.9644454
TAGTACG750.004106761414.84
TCTTATA57000.014.76754437
TCGTTAC7500.014.55333423
TGGAGTG19000.014.50789455
ACCGTCG9450.014.4867738
AACAGTG31600.014.3433542
CGTCGTA9150.014.15300510
TAGTACC2750.014.1272744
ATACCGT10250.014.0780496
CGAGCCG8700.013.82183815
ACGAACG7900.013.81645615
CGCATCG7450.013.65771813
Produced by FastQC (version 0.11.5)