##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727233.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3953523
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174979885029124	31.0	31.0	33.0	30.0	34.0
2	31.38798003704544	31.0	31.0	34.0	30.0	34.0
3	31.531792024480445	31.0	31.0	34.0	30.0	34.0
4	35.35331829358271	37.0	35.0	37.0	33.0	37.0
5	35.17489312696549	37.0	35.0	37.0	32.0	37.0
6	35.18983372551519	37.0	35.0	37.0	32.0	37.0
7	35.07758497927039	37.0	35.0	37.0	32.0	37.0
8	35.109859990696904	37.0	35.0	37.0	32.0	37.0
9	36.63588273041538	39.0	35.0	39.0	32.0	39.0
10	36.37913754390704	38.0	35.0	39.0	32.0	39.0
11	36.54689500984312	38.0	35.0	39.0	32.0	39.0
12	36.41449638714635	38.0	35.0	39.0	32.0	39.0
13	36.4815168648317	38.0	35.0	39.0	32.0	39.0
14	37.44581857750669	39.0	36.0	41.0	32.0	41.0
15	37.447667561311775	39.0	36.0	41.0	32.0	41.0
16	37.396030831235834	39.0	36.0	41.0	32.0	41.0
17	37.430272696023266	39.0	36.0	41.0	32.0	41.0
18	37.38858228471164	39.0	36.0	41.0	32.0	41.0
19	37.421618642410834	39.0	36.0	41.0	32.0	41.0
20	37.35765569088633	39.0	36.0	41.0	31.0	41.0
21	37.264403925309146	39.0	36.0	41.0	31.0	41.0
22	37.19919221413408	39.0	36.0	40.0	31.0	41.0
23	37.08440320190372	39.0	36.0	40.0	31.0	41.0
24	36.951377037644654	39.0	36.0	40.0	31.0	41.0
25	36.81763657375966	39.0	35.0	40.0	30.0	41.0
26	36.56573921537829	38.0	35.0	40.0	30.0	41.0
27	36.37851177291747	38.0	35.0	40.0	30.0	41.0
28	36.14590202206993	38.0	35.0	40.0	29.0	41.0
29	36.05635353582109	38.0	35.0	40.0	29.0	41.0
30	35.92991010802264	38.0	34.0	40.0	29.0	41.0
31	35.96540225009441	38.0	35.0	40.0	29.0	41.0
32	35.89665166991567	38.0	34.0	40.0	29.0	41.0
33	35.808600582316075	38.0	34.0	40.0	29.0	41.0
34	35.82027624475689	38.0	34.0	40.0	29.0	41.0
35	35.57153885281558	38.0	34.0	40.0	27.0	41.0
36	35.48538961326392	38.0	34.0	40.0	27.0	41.0
37	35.35478154547223	38.0	34.0	40.0	26.0	41.0
38	35.209815144619114	38.0	34.0	40.0	26.0	41.0
39	35.07680137436914	38.0	33.0	40.0	25.0	41.0
40	34.816474825111676	38.0	33.0	40.0	24.0	41.0
41	34.677532165615325	38.0	33.0	40.0	24.0	41.0
42	34.38700748674031	38.0	33.0	40.0	23.0	41.0
43	33.99151288610184	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	10.0
15	23.0
16	80.0
17	167.0
18	319.0
19	734.0
20	1541.0
21	3111.0
22	5502.0
23	9935.0
24	16478.0
25	25321.0
26	37538.0
27	53439.0
28	73820.0
29	97458.0
30	124428.0
31	154199.0
32	186878.0
33	227733.0
34	277054.0
35	334083.0
36	412213.0
37	536721.0
38	672207.0
39	702527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.240320089196395	19.16789152358542	13.487944802648169	24.103843584570015
2	17.657289460564666	23.82313192562684	34.377111249890284	24.142467363918207
3	20.452720269997165	25.47740331850858	30.394182606247643	23.67569380524661
4	14.815140825031245	15.9485603093747	33.47035036851942	35.76594849707463
5	13.975181123266514	39.02807698349042	32.36194654742112	14.63479534582194
6	33.545498533839314	34.63741073467892	16.30750598896225	15.50958474251952
7	27.28009929371854	32.616504317794536	21.534616087980265	18.568780300506663
8	28.610836461555934	33.159564267110625	20.08982874261766	18.139770528715783
9	28.37618498741502	13.416818366808538	20.085655249760784	38.12134139601566
10	17.38188951980297	28.098103893666483	31.31720746281228	23.20279912371826
11	34.85498882895078	23.597333315121727	20.433496908959427	21.11418094696806
12	21.290024112671155	27.10734198333992	29.201246584375507	22.40138731961342
13	30.051273256788946	19.847412042373346	25.555156755127012	24.5461579457107
14	22.86770558815517	21.68643005238619	26.43442317143469	29.011441188023944
15	25.52452584694714	27.961618030298546	23.56839709798071	22.9454590247736
16	22.975685230615834	26.703449050378612	26.309395443001087	24.011470276004466
17	22.7983497250427	27.102839669833717	26.6557194684336	23.443091136689986
18	22.330311471565995	25.26038168995096	28.962775731923145	23.446531106559895
19	22.865960309324116	26.439279599486333	29.369400405663505	21.32535968552605
20	22.599817934535857	25.3891276211116	28.931386006860212	23.07966843749233
21	23.49469068473865	26.26788815949724	28.982049680753093	21.255371475011025
22	23.926331021724167	25.558596724996917	27.66497121681093	22.850101036467983
23	22.06477615028419	26.366812587153284	28.59439542908945	22.974015833473082
24	21.926317363020274	26.996352367243087	28.568469185584604	22.508861084152034
25	22.72178510154108	26.04236778184925	27.720592494339858	23.515254622269808
26	22.1954444175486	27.03717671555218	28.584960805843295	22.182418061055927
27	23.18071755242097	26.86237059958928	27.619113383177485	22.33779846481227
28	21.75128360199245	27.784156055244903	28.392018966375055	22.07254137638759
29	21.960312359381746	27.51540840915811	28.903613308939903	21.620665922520242
30	22.447498092207887	27.72185718914497	28.334500646638457	21.49614407200869
31	22.38722273779614	27.559166849415067	27.961061564584295	22.092548848204498
32	21.488935311619535	27.77469613810265	27.894538617835284	22.841829932442533
33	21.126752013330897	27.230826784111283	28.583746698830385	23.058674503727435
34	22.126037966643928	27.475317583835984	28.725771925444725	21.672872524075363
35	21.961981756524498	27.231889127747582	28.779622630246493	22.02650648548143
36	21.09118879541108	27.62966093785214	28.682443481421505	22.596706785315273
37	21.305731622150674	27.297729139301836	29.06101722438443	22.335522014163065
38	21.291592334229495	26.524520029351038	29.127160762691908	23.05672687372756
39	21.034682231518573	26.396962911307202	29.673407742916886	22.894947114257334
40	21.501632847462883	26.4311602588375	29.87512656433262	22.192080329367
41	20.26572249611296	26.89813616867791	30.311926856123005	22.524214479086123
42	20.771625712054792	27.00146173425575	30.039334537828665	22.18757801586079
43	19.919752585225886	26.259945876121122	29.864705479138483	23.955596059514512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2846.0
1	2775.0
2	2704.0
3	4856.5
4	7009.0
5	7009.0
6	10332.5
7	13656.0
8	13126.0
9	12596.0
10	17325.0
11	22054.0
12	22054.0
13	38977.5
14	55901.0
15	83909.5
16	111918.0
17	95457.5
18	78997.0
19	78997.0
20	79404.5
21	79812.0
22	61265.0
23	42718.0
24	48372.0
25	54026.0
26	54026.0
27	58990.0
28	63954.0
29	68675.5
30	73397.0
31	78033.0
32	82669.0
33	82669.0
34	92455.5
35	102242.0
36	119106.5
37	135971.0
38	153663.0
39	171355.0
40	171355.0
41	189294.5
42	207234.0
43	221449.5
44	235665.0
45	262001.5
46	288338.0
47	288338.0
48	315081.0
49	341824.0
50	339691.0
51	337558.0
52	319606.5
53	301655.0
54	301655.0
55	286215.5
56	270776.0
57	255766.5
58	240757.0
59	220362.5
60	199968.0
61	199968.0
62	168310.5
63	136653.0
64	119399.0
65	102145.0
66	85071.0
67	67997.0
68	67997.0
69	55899.5
70	43802.0
71	36600.0
72	29398.0
73	21702.0
74	14006.0
75	14006.0
76	10994.0
77	7982.0
78	6938.0
79	5894.0
80	4644.5
81	3395.0
82	3395.0
83	2787.0
84	2179.0
85	1891.5
86	1604.0
87	1025.0
88	446.0
89	446.0
90	337.5
91	229.0
92	160.0
93	91.0
94	90.5
95	90.0
96	90.0
97	48.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3953523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.88780798333807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7443225709812	37.051799698818954
2	9.589783200222682	8.801082601873194
3	3.3225912497384136	4.573992878253468
4	1.658295018186203	3.0438209349701837
5	1.0147283899850017	2.328183075743677
6	0.6836447713690424	1.8822575998437407
7	0.46784201729078423	1.5027771259183924
8	0.374795559675906	1.375883734033256
9	0.26815374379679185	1.1074488754824374
>10	1.657896291873	13.912333744052408
>50	0.11563854471475557	3.6641397399386997
>100	0.08299546086554027	7.94501430215747
>500	0.0127832231629465	4.086478522304626
>1k	0.006142587739710578	4.843438826466272
>5k	1.6601588485704266E-4	0.48766029771020525
>10k+	2.2135451314272354E-4	3.393688042433085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50812	1.2852334487493813	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32575	0.8239486655319825	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30866	0.7807213970931749	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19390	0.4904486454233351	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	8778	0.22202981998587085	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5312	0.13436117609534584	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5114	0.1293529846670931	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	4827	0.12209363648573691	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4759	0.12037365155078142	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4191	0.10600671856468269	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4053	0.10251616090256715	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4015	0.10155499285068026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.5293896102286493E-5
7	0.0	0.0	0.0	0.0	2.5293896102286493E-5
8	0.0	0.0	0.0	0.0	5.058779220457299E-5
9	0.0	0.0	0.0	0.0	5.058779220457299E-5
10	0.0	0.0	0.0	0.0	5.058779220457299E-5
11	0.0	0.0	0.0	7.588168830685948E-5	7.588168830685948E-5
12	0.0	0.0	0.0	1.0117558440914597E-4	7.588168830685948E-5
13	0.0	0.0	0.0	1.2646948051143247E-4	7.588168830685948E-5
14	0.0	0.0	0.0	4.047023376365839E-4	1.2646948051143247E-4
15	0.0	0.0	0.0	7.588168830685947E-4	1.5176337661371896E-4
16	0.0	0.0	0.0	0.0014164581817280436	2.0235116881829195E-4
17	0.0	0.0	0.0	0.002402920129717217	2.0235116881829195E-4
18	0.0	0.0	0.0	0.002782328571251514	2.782328571251514E-4
19	0.0	0.0	0.0	0.0037687905192406875	2.782328571251514E-4
20	0.0	0.0	0.0	0.005084073116559585	3.288206493297244E-4
21	0.0	0.0	0.0	0.007031703116435645	3.541145454320109E-4
22	0.0	0.0	0.0	0.010901669220085479	3.541145454320109E-4
23	0.0	0.0	0.0	0.017629845583293686	3.541145454320109E-4
24	0.0	0.0	0.0	0.0245350792192179	3.541145454320109E-4
25	0.0	0.0	0.0	0.028076224673538007	3.541145454320109E-4
26	0.0	0.0	0.0	0.035386160647098805	3.794084415342974E-4
27	0.0	0.0	0.0	0.0681670499956621	3.794084415342974E-4
28	0.0	0.0	0.0	0.13342530193956126	3.794084415342974E-4
29	0.0	0.0	0.0	0.2226368734923257	3.794084415342974E-4
30	0.0	0.0	0.0	0.37606964724879555	3.794084415342974E-4
31	0.0	0.0	0.0	0.8198257604673098	4.047023376365839E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3495	0.0	17.891273	37
TATACCG	170	5.456968E-12	17.411764	5
ATACCGA	160	6.311893E-10	16.1875	6
AAGACGG	1045	0.0	15.7559805	5
GGAGTGT	1680	0.0	15.747025	6
GGACCGT	170	1.4897523E-9	15.235293	6
GAACAGT	3110	0.0	15.168811	1
CAAGACG	1125	0.0	14.964445	4
TAGTACG	75	0.0041067614	14.8	4
TCTTATA	5700	0.0	14.767544	37
TCGTTAC	750	0.0	14.553334	23
TGGAGTG	1900	0.0	14.5078945	5
ACCGTCG	945	0.0	14.486773	8
AACAGTG	3160	0.0	14.343354	2
CGTCGTA	915	0.0	14.153005	10
TAGTACC	275	0.0	14.127274	4
ATACCGT	1025	0.0	14.078049	6
CGAGCCG	870	0.0	13.821838	15
ACGAACG	790	0.0	13.816456	15
CGCATCG	745	0.0	13.657718	13
>>END_MODULE