FastQCFastQC Report
Thu 9 Feb 2017
SRR2727232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5769319
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT760891.3188558302981686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT487190.8444497522151228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT438900.7607483656216617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270530.46891149544686295No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG165780.2873476054972866No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC160920.27892373432635637No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA147740.2560787503689777No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA97060.16823476046306332No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG91890.15927356417629188No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86360.14968837743241445No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT81090.14055385046311358No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG80010.13868187909179575No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT75140.1302406748526126No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT73430.12727672018135935No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66990.11611422422646417No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT66560.11536890229158762No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63270.10966632283636941No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT61610.10678903350638091No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59640.10337441906055117No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA59020.10229976882886872No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC58260.10098245564164506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15700.017.0859875
CAAGACG16650.016.7777794
CTTATAC55200.016.72373237
TCGTTAC7800.016.36538523
GAACAGT52450.015.4489981
ACGGACC16900.015.2159778
GACGGAC16850.015.1513357
CGGACCA17950.014.3259069
AACAGTG56500.014.210622
AGACGGA19500.013.6615396
GGAGTGT15850.013.6561536
CTAGCGG10200.013.602941529
TCTAGCG10100.013.55445528
CGCAAGA19800.013.5479782
ACAGTGG61100.013.5343693
CGCTTCG12150.013.39917732
CGAACGA9850.013.33502516
ACGAACG9750.013.28205215
GGCGTCA8150.013.16564412
AGAGCGA20100.013.06965215
Produced by FastQC (version 0.11.5)