##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5769319
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1453072711008	31.0	31.0	33.0	30.0	34.0
2	31.34304412704515	31.0	31.0	34.0	30.0	34.0
3	31.50503794295306	31.0	31.0	34.0	30.0	34.0
4	35.321406217960906	37.0	35.0	37.0	33.0	37.0
5	35.143117757919086	37.0	35.0	37.0	32.0	37.0
6	35.17765216310625	37.0	35.0	37.0	32.0	37.0
7	35.05677723835343	37.0	35.0	37.0	32.0	37.0
8	35.094475275158125	37.0	35.0	37.0	32.0	37.0
9	36.628160966658285	39.0	35.0	39.0	32.0	39.0
10	36.37597609007233	38.0	35.0	39.0	32.0	39.0
11	36.53049519362684	38.0	35.0	39.0	32.0	39.0
12	36.40180028180102	38.0	35.0	39.0	32.0	39.0
13	36.461033962587265	38.0	35.0	39.0	32.0	39.0
14	37.42114849256905	39.0	36.0	41.0	32.0	41.0
15	37.41832164246768	39.0	36.0	41.0	32.0	41.0
16	37.35622644544356	39.0	36.0	41.0	32.0	41.0
17	37.41241418614571	39.0	36.0	41.0	32.0	41.0
18	37.360154465371046	39.0	36.0	41.0	32.0	41.0
19	37.38993232996823	39.0	36.0	41.0	32.0	41.0
20	37.34361490498272	39.0	36.0	41.0	31.0	41.0
21	37.24553625826549	39.0	36.0	41.0	31.0	41.0
22	37.19170668149915	39.0	36.0	40.0	31.0	41.0
23	37.07822465701758	39.0	36.0	40.0	31.0	41.0
24	36.95160243349345	39.0	36.0	40.0	31.0	41.0
25	36.82431982006889	39.0	35.0	40.0	30.0	41.0
26	36.5724547385922	38.0	35.0	40.0	30.0	41.0
27	36.38441209439104	38.0	35.0	40.0	30.0	41.0
28	36.1668831278007	38.0	35.0	40.0	29.0	41.0
29	36.08192734705777	38.0	35.0	40.0	29.0	41.0
30	35.937452929886526	38.0	35.0	40.0	29.0	41.0
31	35.97126073285253	38.0	35.0	40.0	30.0	41.0
32	35.84984206974861	38.0	34.0	40.0	29.0	41.0
33	35.78410224846294	38.0	34.0	40.0	29.0	41.0
34	35.78699219093276	38.0	34.0	40.0	29.0	41.0
35	35.53785204111612	38.0	34.0	40.0	27.0	41.0
36	35.45962443747693	38.0	34.0	40.0	27.0	41.0
37	35.313812774089975	38.0	34.0	40.0	26.0	41.0
38	35.172249792393174	38.0	34.0	40.0	25.0	41.0
39	35.05088607511563	38.0	33.0	40.0	25.0	41.0
40	34.79639832708158	38.0	33.0	40.0	24.0	41.0
41	34.66216324665008	38.0	33.0	40.0	24.0	41.0
42	34.37175271466182	38.0	33.0	40.0	23.0	41.0
43	33.95880830995824	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	10.0
14	3.0
15	41.0
16	110.0
17	261.0
18	555.0
19	1152.0
20	2426.0
21	4592.0
22	8584.0
23	14819.0
24	23859.0
25	37187.0
26	55406.0
27	79012.0
28	109101.0
29	143457.0
30	181741.0
31	225229.0
32	273781.0
33	330472.0
34	403742.0
35	486430.0
36	604073.0
37	794134.0
38	993018.0
39	996123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84536545821093	18.932910452689477	13.08603320426553	24.13569088483407
2	17.10505174007539	24.879816838001155	33.56749730774117	24.447634114182282
3	20.58998644380732	26.410708092237577	29.90418799861821	23.095117465336898
4	14.849291571500899	16.313970505011078	33.3164278140973	35.52031010939073
5	13.785405175203522	39.1318282105739	32.595042846478066	14.487723767744512
6	33.46188692287599	34.481972655698186	16.51295412855486	15.543186292870962
7	26.992596526557122	32.24496686697338	22.172131580867692	18.590305025601808
8	28.91446980137517	32.50624553781824	20.811087062441857	17.76819759836473
9	28.408968198846345	13.178071796688656	20.004388732881644	38.408571271583355
10	17.273997156336822	28.091201058565147	31.82760391651077	22.807197868587263
11	34.64140221748875	23.526502868016138	20.467025657620944	21.365069256874165
12	21.371326494513475	27.53179361376967	29.427684619276555	21.6691952724403
13	30.364432959938597	19.609125444441535	25.42788845615921	24.598553139460655
14	22.728644403264926	21.701122784162223	26.628220765743755	28.942012046829095
15	25.792246883904323	27.46701993770842	23.78991350625611	22.950819672131146
16	22.757087968268007	26.3690393961575	26.90190991345772	23.971962722116768
17	23.135052854591677	27.075933918717272	27.021768080426824	22.76724514626423
18	22.92097212859958	24.39436959544099	29.402447671900273	23.282210604059163
19	22.644301693146108	27.06863669698278	29.422796694029223	20.864264915841886
20	22.61310217029081	25.42700446967831	29.27615893660933	22.68373442342155
21	23.16169378049645	26.674881385480674	28.85628269125004	21.307142142772832
22	23.354385500264417	26.33924385183069	27.30700105159725	22.999369596307638
23	21.43100424850836	26.543947387897948	28.48133375880238	23.54371460479131
24	21.624527955552466	28.165057262390935	28.17862905483299	22.031785727223614
25	22.45774587953968	26.26257969094793	27.368949437533267	23.910724991979123
26	22.1082938904921	27.19166681544217	28.598227971100226	22.1018113229655
27	23.055563403583683	26.862841177615593	27.398606317314055	22.682989101486676
28	21.513249657368576	27.78866968527828	28.78975837529525	21.9083222820579
29	22.05220408162558	27.268348309393186	28.984044737342487	21.69540287163875
30	21.756640601776397	28.414428115346023	28.794455636791795	21.034475646085784
31	22.601281017742302	27.49995276738901	27.43831637668155	22.46044983818714
32	20.645278931534207	28.161139988965765	28.17916637994883	23.014414699551196
33	20.963808726818538	27.577414249411415	28.72117142421835	22.737605599551696
34	21.54703180739356	27.650022472322988	28.767554714863223	22.035391005420223
35	21.547725130123677	27.922792967419554	29.163043333190625	21.366438569266148
36	21.277814591288852	27.902634609041378	28.430773198708547	22.388777600961223
37	21.132372815578407	27.61161239307447	28.961321084862874	22.294693706484246
38	21.240565827613274	27.02138675292526	29.189146933979554	22.54890048548191
39	21.33204976185231	26.133517664736516	29.23982882555116	23.294603747860016
40	21.227618025628328	26.878995597227334	29.77113936670862	22.122247010435718
41	20.150402499844436	26.96791423736493	30.242408159437883	22.63927510335275
42	21.09245475939188	27.250859243525973	29.78523115119826	21.87145484588389
43	20.150870492687265	26.033471194780528	30.109186196845762	23.706472115686445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2528.0
1	2565.0
2	2602.0
3	6492.0
4	10382.0
5	10382.0
6	16149.0
7	21916.0
8	21261.0
9	20606.0
10	26735.0
11	32864.0
12	32864.0
13	56150.0
14	79436.0
15	122735.5
16	166035.0
17	144715.5
18	123396.0
19	123396.0
20	124392.0
21	125388.0
22	95156.0
23	64924.0
24	70244.5
25	75565.0
26	75565.0
27	82662.5
28	89760.0
29	97062.0
30	104364.0
31	110639.0
32	116914.0
33	116914.0
34	129858.5
35	142803.0
36	167780.5
37	192758.0
38	220026.5
39	247295.0
40	247295.0
41	271209.5
42	295124.0
43	316179.5
44	337235.0
45	376336.0
46	415437.0
47	415437.0
48	491794.0
49	568151.0
50	565117.5
51	562084.0
52	487864.5
53	413645.0
54	413645.0
55	391346.0
56	369047.0
57	350629.0
58	332211.0
59	305672.5
60	279134.0
61	279134.0
62	233577.5
63	188021.0
64	162907.0
65	137793.0
66	116105.5
67	94418.0
68	94418.0
69	78057.0
70	61696.0
71	52412.0
72	43128.0
73	31577.0
74	20026.0
75	20026.0
76	15784.0
77	11542.0
78	10112.5
79	8683.0
80	6865.0
81	5047.0
82	5047.0
83	4228.5
84	3410.0
85	3016.5
86	2623.0
87	1680.5
88	738.0
89	738.0
90	520.5
91	303.0
92	222.0
93	141.0
94	137.5
95	134.0
96	134.0
97	69.5
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5769319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.88732026902854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.82106982472445	35.90907496152229
2	9.359643276887319	8.215393241932272
3	3.099906263515609	4.081397369726314
4	1.5348262631377811	2.694376470705764
5	0.8619999593315602	1.8915434143536878
6	0.5967918500411259	1.5714957034020574
7	0.4169682754153541	1.2809734171624703
8	0.3257384655860135	1.1436630690492249
9	0.25465262391887117	1.005841913694838
>10	1.498930220466126	12.301927743166274
>50	0.12016107605524007	3.63461884889022
>100	0.08470544354807241	7.606806320299089
>500	0.01369230564685886	4.2095301656927635
>1k	0.010080707412187771	8.440202767578436
>5k	5.556295424038071E-4	1.7772028994451234
>10k+	2.7781477120190353E-4	4.235951693379169
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	76089	1.3188558302981686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	48719	0.8444497522151228	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	43890	0.7607483656216617	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27053	0.46891149544686295	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	16578	0.2873476054972866	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	16092	0.27892373432635637	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	14774	0.2560787503689777	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	9706	0.16823476046306332	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	9189	0.15927356417629188	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8636	0.14968837743241445	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	8109	0.14055385046311358	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	8001	0.13868187909179575	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7514	0.1302406748526126	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	7343	0.12727672018135935	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6699	0.11611422422646417	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	6656	0.11536890229158762	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6327	0.10966632283636941	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6161	0.10678903350638091	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	5964	0.10337441906055117	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	5902	0.10229976882886872	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	5826	0.10098245564164506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.199920475882855E-5
2	0.0	0.0	0.0	1.7333068252942853E-5	5.199920475882855E-5
3	0.0	0.0	0.0	1.7333068252942853E-5	5.199920475882855E-5
4	0.0	0.0	0.0	1.7333068252942853E-5	5.199920475882855E-5
5	0.0	0.0	0.0	1.7333068252942853E-5	5.199920475882855E-5
6	0.0	0.0	0.0	1.7333068252942853E-5	1.039984095176571E-4
7	0.0	0.0	0.0	1.7333068252942853E-5	1.039984095176571E-4
8	0.0	0.0	0.0	1.7333068252942853E-5	1.039984095176571E-4
9	0.0	0.0	0.0	1.7333068252942853E-5	1.3866454602354283E-4
10	0.0	0.0	0.0	3.4666136505885707E-5	1.3866454602354283E-4
11	0.0	0.0	0.0	5.199920475882855E-5	1.5599761427648567E-4
12	0.0	0.0	0.0	6.933227301177141E-5	1.5599761427648567E-4
13	0.0	0.0	0.0	1.039984095176571E-4	1.5599761427648567E-4
14	0.0	0.0	0.0	2.4266295554119992E-4	2.079968190353142E-4
15	0.0	0.0	0.0	6.066573888529999E-4	2.599960237941428E-4
16	0.0	0.0	0.0	0.001889304439570771	3.293282968059142E-4
17	0.0	0.0	0.0	0.003050620012517942	3.46661365058857E-4
18	0.0	0.0	0.0	0.003778608879141542	3.639944333117999E-4
19	0.0	0.0	0.0	0.005737245591724084	3.639944333117999E-4
20	0.0	0.0	0.0	0.007227889461477169	3.639944333117999E-4
21	0.0	0.0	0.0	0.012046482435795283	3.639944333117999E-4
22	1.7333068252942853E-5	0.0	0.0	0.01797439177830174	3.639944333117999E-4
23	1.7333068252942853E-5	0.0	0.0	0.026900921928567304	3.8132750156474274E-4
24	1.7333068252942853E-5	0.0	0.0	0.039051402773880244	3.8132750156474274E-4
25	1.7333068252942853E-5	0.0	0.0	0.0438873288164513	3.8132750156474274E-4
26	1.7333068252942853E-5	0.0	0.0	0.056020476593511295	4.333267063235713E-4
27	1.7333068252942853E-5	0.0	0.0	0.09794916869738006	4.333267063235713E-4
28	3.4666136505885707E-5	0.0	0.0	0.17606930731339349	4.333267063235713E-4
29	3.4666136505885707E-5	0.0	0.0	0.27173051100138507	4.333267063235713E-4
30	3.4666136505885707E-5	0.0	0.0	0.4245388407193293	4.333267063235713E-4
31	3.4666136505885707E-5	0.0	0.0	0.8425604477755521	4.5065977457651416E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1570	0.0	17.085987	5
CAAGACG	1665	0.0	16.777779	4
CTTATAC	5520	0.0	16.723732	37
TCGTTAC	780	0.0	16.365385	23
GAACAGT	5245	0.0	15.448998	1
ACGGACC	1690	0.0	15.215977	8
GACGGAC	1685	0.0	15.151335	7
CGGACCA	1795	0.0	14.325906	9
AACAGTG	5650	0.0	14.21062	2
AGACGGA	1950	0.0	13.661539	6
GGAGTGT	1585	0.0	13.656153	6
CTAGCGG	1020	0.0	13.6029415	29
TCTAGCG	1010	0.0	13.554455	28
CGCAAGA	1980	0.0	13.547978	2
ACAGTGG	6110	0.0	13.534369	3
CGCTTCG	1215	0.0	13.399177	32
CGAACGA	985	0.0	13.335025	16
ACGAACG	975	0.0	13.282052	15
GGCGTCA	815	0.0	13.165644	12
AGAGCGA	2010	0.0	13.069652	15
>>END_MODULE