FastQCFastQC Report
Thu 9 Feb 2017
SRR2727231.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727231.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4354622
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT551591.2666771076800696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT361370.8298538885809148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT320470.7359306961660507No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209010.4799727737562525No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA105040.24121496653440871No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG77280.1774666090420707No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC77250.177397716724896No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66400.15248166201337338No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT55280.1269455764472783No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51790.11893110354928625No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA45690.10492299905709382No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG44540.10228212689872968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG11450.016.6419225
CAAGACG11750.016.0595744
CTTATAC40450.015.41285637
TACCCCG750.00410685114.85
GACGGAC12700.014.421267
GAACAGT36500.014.2424651
CTAGACA3650.014.1917814
AGGGCGT7850.014.14012710
TACGGAT11050.013.89592627
TATACCG1203.304516E-513.8749995
GGGCGTC7750.013.84516211
ACGGACC13150.013.7870728
GGCGTCA7550.013.72185512
TCGTTAC6500.013.66153823
TAGACAG5700.013.6315795
AACAGTG37200.013.5766122
GCGTTAT9850.013.3350261
CGTTACG5850.013.28205124
CGTTATA1409.572645E-613.2142852
CGGACCA13750.013.1854559
Produced by FastQC (version 0.11.5)