FastQCFastQC Report
Thu 9 Feb 2017
SRR2727230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649484
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT168451.0212284568992485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112630.682819596916369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98200.5953376934847504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66180.40121638039532365No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA35260.21376381947324133No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.15277504965189112No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG24770.15016817380465647No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC24330.1475006729377187No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT21450.13004066726321686No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.10797316009127703No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.10300190847562025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG5050.019.4158425
CAAGACG5400.018.8425924
ACCGTCG3900.018.025648
CGTCGTA4050.017.81481610
CCGTCGT3950.017.3291139
GTCGTAG4100.017.14634311
AGACGGA6000.016.9583346
ACACCGT904.4478427E-516.4444456
TACCGTC4450.016.2134847
TCGTTAC2750.016.14545423
CGGACCA6100.016.073779
GCGCAAG6250.015.9841
ATACCGT4650.015.9139796
ACGGACC6300.015.5634928
CGCAAGA6700.015.4626872
CTTATAC18150.015.28925637
TAGAGTG4000.015.2625015
ATCTCGT4500.015.21111120
ATTATAC1101.4525507E-515.1363643
GACGGAC6500.015.0846177
Produced by FastQC (version 0.11.5)