##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727230.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1649484
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16826898593742	31.0	31.0	33.0	30.0	34.0
2	31.38513923142025	31.0	31.0	34.0	30.0	34.0
3	31.546661865165106	31.0	31.0	34.0	30.0	34.0
4	35.354511471466225	37.0	35.0	37.0	33.0	37.0
5	35.17785501405288	37.0	35.0	37.0	32.0	37.0
6	35.19428015064105	37.0	35.0	37.0	32.0	37.0
7	35.07901501317988	37.0	35.0	37.0	32.0	37.0
8	35.10449813396189	37.0	35.0	37.0	32.0	37.0
9	36.65798455759498	39.0	35.0	39.0	32.0	39.0
10	36.37953687334949	38.0	35.0	39.0	32.0	39.0
11	36.5520950794309	38.0	35.0	39.0	32.0	39.0
12	36.417369916895225	38.0	35.0	39.0	32.0	39.0
13	36.48852125876941	38.0	35.0	39.0	32.0	39.0
14	37.46783115204512	39.0	36.0	41.0	32.0	41.0
15	37.46463803225736	39.0	36.0	41.0	32.0	41.0
16	37.40080413026134	39.0	36.0	41.0	32.0	41.0
17	37.42698989502171	39.0	36.0	41.0	32.0	41.0
18	37.37147010822778	39.0	36.0	41.0	32.0	41.0
19	37.39980078618525	39.0	36.0	41.0	32.0	41.0
20	37.32332232382976	39.0	36.0	41.0	31.0	41.0
21	37.224573866736506	39.0	36.0	41.0	31.0	41.0
22	37.16947178632833	39.0	36.0	40.0	31.0	41.0
23	37.047484546682476	39.0	36.0	40.0	31.0	41.0
24	36.91111523361245	39.0	36.0	40.0	31.0	41.0
25	36.77794934658354	39.0	35.0	40.0	30.0	41.0
26	36.529387978301095	38.0	35.0	40.0	30.0	41.0
27	36.34436466191851	38.0	35.0	40.0	30.0	41.0
28	36.08877442885169	38.0	35.0	40.0	29.0	41.0
29	36.01640634283206	38.0	35.0	40.0	29.0	41.0
30	35.88530291897345	38.0	34.0	40.0	29.0	41.0
31	35.94324285655393	38.0	34.0	40.0	29.0	41.0
32	35.849143125971516	38.0	34.0	40.0	29.0	41.0
33	35.78860176879557	38.0	34.0	40.0	29.0	41.0
34	35.79638238382427	38.0	34.0	40.0	28.0	41.0
35	35.5513396916854	38.0	34.0	40.0	27.0	41.0
36	35.47142803446411	38.0	34.0	40.0	27.0	41.0
37	35.356127734491515	38.0	34.0	40.0	26.0	41.0
38	35.21360437567142	38.0	34.0	40.0	26.0	41.0
39	35.10253873332509	38.0	33.0	40.0	25.0	41.0
40	34.872748083643124	38.0	33.0	40.0	24.0	41.0
41	34.74101658458039	38.0	33.0	40.0	24.0	41.0
42	34.47751175519132	38.0	33.0	40.0	23.0	41.0
43	34.07904775069052	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	4.0
15	7.0
16	37.0
17	82.0
18	149.0
19	306.0
20	655.0
21	1297.0
22	2389.0
23	4114.0
24	6997.0
25	10531.0
26	15811.0
27	22634.0
28	31111.0
29	40982.0
30	51805.0
31	64059.0
32	78095.0
33	94305.0
34	115614.0
35	139263.0
36	171482.0
37	223116.0
38	283083.0
39	291554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64631606005272	18.964415538435052	12.80121541039501	24.58805299111722
2	17.46867505231939	24.175924107175334	33.99560104857034	24.359799791934933
3	20.17618843226124	26.108225360173243	29.944879732085912	23.770706475479606
4	14.684410397433378	15.871327033181284	33.945949157433475	35.49831341195186
5	13.991527047246292	38.663060690494724	32.78322190454712	14.562190357711865
6	33.63124468015452	34.74456254198282	16.461208474892754	15.162984302969898
7	27.4181501608988	32.33156550775879	22.123342815086414	18.126941516255993
8	28.346137337494635	32.95570008560253	20.85900802917761	17.83915454772523
9	28.716071207723143	13.335928084176626	19.86275708039605	38.08524362770418
10	17.140451195646637	27.25822135892194	32.09264230510875	23.508685140322672
11	35.274607089247304	23.212713794131982	20.7794680033271	20.733211113293613
12	21.408816332865307	26.662338040259865	29.47242895353941	22.45641667333542
13	29.772644051109314	19.515193842438	25.639412082808928	25.07275002364376
14	22.672787368655893	21.535946999182777	26.259484784332553	29.531780847828777
15	25.687366473394103	27.71939588380366	23.381372598946097	23.21186504385614
16	23.198042539363826	26.57521988694646	26.19958726486586	24.02715030882385
17	23.197254414107686	26.831481845231597	26.343450436621392	23.627813304039325
18	23.033445610869823	25.070143147796525	28.57996803848961	23.31644320284404
19	23.359123216715044	26.61256489908359	28.577482412681782	21.45082947151958
20	22.862786180405507	25.28554384280175	28.29915294722471	23.552517029568033
21	23.77398022654357	26.599288019768608	28.233496050886213	21.3932357028016
22	24.03472843628674	25.672695218625947	26.781405578956814	23.511170766130498
23	21.840223973072792	26.34569356235041	28.081569751510173	23.73251271306663
24	22.01870403107881	27.229181974484142	27.89799719184909	22.854116802587964
25	22.954754335295156	25.959027186683837	26.97049501541088	24.115723462610124
26	22.463267300561874	27.01372065445921	28.01942910631446	22.503582938664454
27	23.697168326579707	26.7055636793082	26.833421845862098	22.763846148249996
28	21.940194630563255	27.779475278329464	27.739947765483024	22.540382325624257
29	22.36717664433241	27.348795138358422	28.309337950534836	21.974690266774335
30	22.792461157549877	27.679443995819298	27.80299778597428	21.725097060656545
31	22.86745430692265	27.255371982995896	27.182258209233918	22.694915500847536
32	21.59820889441789	27.70545212927194	27.193291962819888	23.50304701349028
33	21.344250686881473	26.871191233137147	28.044103489333633	23.74045459064774
34	22.369965395238754	27.20669009217428	28.341347960938084	22.08199655164888
35	22.46435855091653	27.096170681255472	28.356019215706247	22.083451552121755
36	21.6525895370916	27.45852642402109	28.073567248909352	22.815316789977956
37	21.736918939498658	27.10872006033402	28.518373018471234	22.635987981696093
38	21.882115861687655	26.37091357054691	28.65932618928101	23.087644378484423
39	21.728431436740216	26.002737825889792	28.84144374846922	23.42738698890077
40	22.16123345240087	26.273004163726355	29.147539472950328	22.41822291092245
41	20.647972335591007	26.6224467772952	29.7045621539827	23.025018733131088
42	21.56898763492098	26.80789871256708	29.310135775794127	22.31297787671781
43	20.50101728783062	25.808434637741257	29.311227026148785	24.37932104827934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	854.0
1	1026.5
2	1199.0
3	2284.0
4	3369.0
5	3369.0
6	4916.5
7	6464.0
8	6089.5
9	5715.0
10	7306.5
11	8898.0
12	8898.0
13	14553.0
14	20208.0
15	29540.5
16	38873.0
17	34305.0
18	29737.0
19	29737.0
20	30136.0
21	30535.0
22	23604.0
23	16673.0
24	18068.0
25	19463.0
26	19463.0
27	21561.0
28	23659.0
29	25726.5
30	27794.0
31	29810.5
32	31827.0
33	31827.0
34	36314.0
35	40801.0
36	48791.0
37	56781.0
38	65722.5
39	74664.0
40	74664.0
41	81221.5
42	87779.0
43	93381.0
44	98983.0
45	110450.0
46	121917.0
47	121917.0
48	137706.5
49	153496.0
50	153662.5
51	153829.0
52	139721.0
53	125613.0
54	125613.0
55	118424.5
56	111236.0
57	106230.5
58	101225.0
59	92733.5
60	84242.0
61	84242.0
62	69764.5
63	55287.0
64	48313.0
65	41339.0
66	34803.0
67	28267.0
68	28267.0
69	23527.0
70	18787.0
71	16032.0
72	13277.0
73	9817.5
74	6358.0
75	6358.0
76	4943.5
77	3529.0
78	3092.5
79	2656.0
80	2160.5
81	1665.0
82	1665.0
83	1398.0
84	1131.0
85	995.5
86	860.0
87	566.0
88	272.0
89	272.0
90	192.0
91	112.0
92	79.0
93	46.0
94	54.5
95	63.0
96	63.0
97	32.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1649484.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.01515082197344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.23213089396255	43.8134699250567
2	8.261429387032518	8.594389911431598
3	2.7395161490945057	4.2748903702314776
4	1.3619805606855586	2.8337449712262113
5	0.8150801096148069	2.1198257416802413
6	0.5159873867748227	1.6103497047197008
7	0.36120312730164156	1.315162460077435
8	0.2641255917577042	1.0990825992975974
9	0.195099007730864	0.913329388110443
>10	1.0451345691349114	9.876130441124484
>50	0.0982229546964719	3.5912512970560364
>100	0.09545073814443679	10.509661332592247
>500	0.009837760174638558	3.4480290115757803
>1k	0.004333299124543174	3.2870223036095823
>5k	2.3423238511044182E-4	1.0013727830300292
>10k+	2.3423238511044182E-4	1.7122877591804393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16845	1.0212284568992485	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11263	0.682819596916369	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9820	0.5953376934847504	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6618	0.40121638039532365	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3526	0.21376381947324133	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2520	0.15277504965189112	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	2477	0.15016817380465647	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	2433	0.1475006729377187	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2145	0.13004066726321686	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1781	0.10797316009127703	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1699	0.10300190847562025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.06250197031314E-5
2	0.0	0.0	0.0	0.0	6.06250197031314E-5
3	0.0	0.0	0.0	0.0	6.06250197031314E-5
4	0.0	0.0	0.0	0.0	6.06250197031314E-5
5	0.0	0.0	0.0	0.0	6.06250197031314E-5
6	0.0	0.0	0.0	0.0	1.212500394062628E-4
7	0.0	0.0	0.0	6.06250197031314E-5	1.212500394062628E-4
8	0.0	0.0	0.0	6.06250197031314E-5	1.212500394062628E-4
9	0.0	0.0	0.0	6.06250197031314E-5	1.818750591093942E-4
10	0.0	0.0	0.0	1.212500394062628E-4	1.818750591093942E-4
11	0.0	0.0	0.0	3.637501182187884E-4	1.818750591093942E-4
12	0.0	0.0	0.0	6.668752167344454E-4	1.818750591093942E-4
13	0.0	0.0	0.0	7.881252561407082E-4	1.818750591093942E-4
14	0.0	0.0	0.0	0.0012731254137657595	2.425000788125256E-4
15	0.0	0.0	0.0	0.0017581255713908107	3.03125098515657E-4
16	0.0	0.0	0.0	0.003637501182187884	3.03125098515657E-4
17	0.0	0.0	0.0	0.006305002049125666	3.03125098515657E-4
18	0.0	0.0	0.0	0.007396252403782031	3.03125098515657E-4
19	0.0	0.0	0.0	0.010063753270719813	3.03125098515657E-4
20	0.0	0.0	0.0	0.012428129039141938	3.03125098515657E-4
21	0.0	0.0	0.0	0.018248130930642554	3.03125098515657E-4
22	0.0	0.0	0.0	0.027463133925518527	3.03125098515657E-4
23	0.0	0.0	0.0	0.03964876288584794	3.03125098515657E-4
24	0.0	0.0	0.0	0.0575331436982717	3.03125098515657E-4
25	0.0	0.0	0.0	0.06456564598383495	3.03125098515657E-4
26	0.0	0.0	0.0	0.07941877581110214	3.637501182187884E-4
27	0.0	0.0	0.0	0.14022567057334293	3.637501182187884E-4
28	0.0	0.0	0.0	0.23680132696043127	3.637501182187884E-4
29	0.0	0.0	0.0	0.34950323858855253	3.637501182187884E-4
30	0.0	0.0	0.0	0.5187682935996955	3.637501182187884E-4
31	0.0	0.0	0.0	0.9550865604031321	3.637501182187884E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	505	0.0	19.415842	5
CAAGACG	540	0.0	18.842592	4
ACCGTCG	390	0.0	18.02564	8
CGTCGTA	405	0.0	17.814816	10
CCGTCGT	395	0.0	17.329113	9
GTCGTAG	410	0.0	17.146343	11
AGACGGA	600	0.0	16.958334	6
ACACCGT	90	4.4478427E-5	16.444445	6
TACCGTC	445	0.0	16.213484	7
TCGTTAC	275	0.0	16.145454	23
CGGACCA	610	0.0	16.07377	9
GCGCAAG	625	0.0	15.984	1
ATACCGT	465	0.0	15.913979	6
ACGGACC	630	0.0	15.563492	8
CGCAAGA	670	0.0	15.462687	2
CTTATAC	1815	0.0	15.289256	37
TAGAGTG	400	0.0	15.262501	5
ATCTCGT	450	0.0	15.211111	20
ATTATAC	110	1.4525507E-5	15.136364	3
GACGGAC	650	0.0	15.084617	7
>>END_MODULE