FastQCFastQC Report
Thu 9 Feb 2017
SRR2727229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285331
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT460951.0756461986250303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT316080.7375859647714494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282010.6580821878169971No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177800.41490377289408914No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA118440.27638471800661374No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66470.15511053871917946No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT55420.12932489929015986No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48360.11285009255994462No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.11133329024059051No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46480.10846303354396661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC953.611036E-617.5263163
AAGACGG12950.017.4285725
CAAGACG14050.016.0640564
GAACAGT39150.015.6883771
ATACCGT10300.015.4466036
TATACCG1455.356378E-815.3103455
TATACTG5000.015.1700015
AACAGTG39350.015.13852
ACCGTCG9350.015.0374338
ACGGACC15250.014.9213118
TACGGAT9650.014.76165927
TACCGTC9800.014.5357157
GACGGAC15300.014.5098047
ACGAACG7400.014.515
CGCAAGA16450.014.2826762
TAATACC4150.014.265064
TCTAGCG8450.014.2307728
CGAACGA7550.014.2119216
AACGAAC7550.014.2119214
CCGTCGT9650.014.1865299
Produced by FastQC (version 0.11.5)