FastQCFastQC Report
Thu 9 Feb 2017
SRR2727228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5530973
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT518740.9378819965311709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346030.6256222910507789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324760.587166127912756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205470.37148979031356694No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA91300.16507041346974574No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT81840.14796673207408534No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71730.12968785058252863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18200.018.3983525
CAAGACG19650.017.2290084
CTTATAC50450.016.53815737
TAATCCC12700.015.5866145
CGCTTCG12950.015.57142832
AGACGGA22100.015.4027156
TATACTG5450.015.2752295
CGCAAGA21750.015.140232
GACGGAC22050.015.1020417
ACGGACC22550.015.0133038
TACGGAT22800.015.01096427
ACGGATG22900.014.8646328
GTTACGG18050.014.86149625
GCGCAAG22750.014.81
TACGTCG750.00410703614.812
CGGACCA22750.014.7186819
TCGTTAC15050.014.50498423
CGTTACG12050.014.43153524
ACTAGAC3350.014.3582093
GTAAACG13750.014.26181827
Produced by FastQC (version 0.11.5)