##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5530973
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200026649922176	31.0	31.0	34.0	30.0	34.0
2	31.40963172302595	31.0	31.0	34.0	30.0	34.0
3	31.56279591312415	31.0	31.0	34.0	30.0	34.0
4	35.37246665279328	37.0	35.0	37.0	33.0	37.0
5	35.19784023534376	37.0	35.0	37.0	32.0	37.0
6	35.21086940760694	37.0	35.0	37.0	32.0	37.0
7	35.10379493806967	37.0	35.0	37.0	32.0	37.0
8	35.12656561512775	37.0	35.0	37.0	32.0	37.0
9	36.67988381067852	39.0	35.0	39.0	32.0	39.0
10	36.41917344380455	38.0	35.0	39.0	32.0	39.0
11	36.57574951098116	39.0	35.0	39.0	32.0	39.0
12	36.43773853171946	38.0	35.0	39.0	32.0	39.0
13	36.5058198620749	38.0	35.0	39.0	32.0	39.0
14	37.497009838956004	39.0	36.0	41.0	32.0	41.0
15	37.500866122470676	39.0	36.0	41.0	32.0	41.0
16	37.43863584219268	39.0	36.0	41.0	32.0	41.0
17	37.45034264314796	39.0	36.0	41.0	32.0	41.0
18	37.392033011189895	39.0	36.0	41.0	32.0	41.0
19	37.41970843104821	39.0	36.0	41.0	32.0	41.0
20	37.34707166352105	39.0	36.0	41.0	31.0	41.0
21	37.24684318654241	39.0	36.0	41.0	31.0	41.0
22	37.17637981599259	39.0	36.0	40.0	31.0	41.0
23	37.060100094504165	39.0	36.0	40.0	31.0	41.0
24	36.925319288306056	39.0	36.0	40.0	31.0	41.0
25	36.79197963902554	39.0	35.0	40.0	30.0	41.0
26	36.56521031652116	38.0	35.0	40.0	30.0	41.0
27	36.376407008314814	38.0	35.0	40.0	30.0	41.0
28	36.135305668640946	38.0	35.0	40.0	29.0	41.0
29	36.07217446188944	38.0	35.0	40.0	29.0	41.0
30	35.961620857668265	38.0	35.0	40.0	29.0	41.0
31	36.0369289454134	38.0	35.0	40.0	30.0	41.0
32	35.97337538982743	38.0	35.0	40.0	29.0	41.0
33	35.924617603448795	38.0	34.0	40.0	29.0	41.0
34	35.95113680721276	38.0	35.0	40.0	29.0	41.0
35	35.70866626902717	38.0	34.0	40.0	27.0	41.0
36	35.63915499135505	38.0	34.0	40.0	27.0	41.0
37	35.534900278847864	38.0	34.0	40.0	27.0	41.0
38	35.3968075779795	38.0	34.0	40.0	26.0	41.0
39	35.290069577269676	38.0	34.0	40.0	26.0	41.0
40	35.06945920726787	38.0	33.0	40.0	25.0	41.0
41	34.95510844113685	38.0	33.0	40.0	25.0	41.0
42	34.702846858952306	38.0	33.0	40.0	24.0	41.0
43	34.33212890390172	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	4.0
14	9.0
15	56.0
16	140.0
17	272.0
18	565.0
19	1048.0
20	2319.0
21	4423.0
22	7916.0
23	13714.0
24	22145.0
25	34750.0
26	50713.0
27	72992.0
28	100570.0
29	132488.0
30	169847.0
31	209992.0
32	255933.0
33	311072.0
34	381835.0
35	460709.0
36	571505.0
37	739377.0
38	958593.0
39	1027980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40138706155318	18.936324585204087	12.644972231829735	25.017316121412996
2	17.768772329931824	23.493009277029557	34.01461189559233	24.72360649744629
3	20.247215092172752	25.326881906673567	30.186316223203402	24.23958677795028
4	14.457546619735803	15.714667202316843	34.22687111291268	35.60091506503467
5	13.90319569450077	38.76330620308578	32.922688286491365	14.410809815922082
6	33.874980044198374	35.04016381927737	16.141084037112456	14.943772099411804
7	27.69689890006695	32.49878457190082	21.58329104119655	18.22102548683568
8	28.487121524549114	33.100776301023345	20.380048863011986	18.03205331141555
9	28.64662329756446	13.144920432625506	19.405120943457867	38.80333532635216
10	17.044849070859684	27.128698693701814	31.959801647919818	23.866650587518688
11	36.11841894726299	22.620414165825796	20.59404376047397	20.667123126437247
12	21.56242671949402	26.25684486255854	29.300070710885773	22.88065770706167
13	30.036378770968508	19.25554509125248	25.594520168512847	25.113555969266166
14	22.672701530092446	21.161791966802223	26.021081643320258	30.14442485978507
15	25.696852976863205	27.71129058124131	23.06107804178397	23.53077840011152
16	23.711108334826438	26.260659742869834	25.854673309741344	24.17355861256238
17	23.404127989776843	26.490221521602074	26.059031566416973	24.046618922204104
18	23.278200779501184	24.893739311329128	28.128830135312537	23.69922977385715
19	23.827308504308373	26.00287508183461	28.182473499689838	21.987342914167183
20	23.345874225023337	24.7173688969373	27.790697947720954	24.14605893031841
21	24.40990039184787	25.942686756923237	27.822898430348513	21.824514420880377
22	24.494171278724377	24.972929717791065	26.699280578661295	23.833618424823264
23	22.44536359154167	25.565447526140517	27.83018467094307	24.159004211374743
24	22.531026638531774	26.27007942363848	27.768242585888597	23.43065135194115
25	23.403007029685373	25.21095293721376	26.936255157998424	24.449784875102445
26	22.814683781678198	26.413562315346685	27.79395234798651	22.977801554988606
27	24.203589495012903	26.029271883988585	26.63444569337077	23.132692927627744
28	22.35340870403815	27.173356297345876	27.351390071873432	23.121844926742547
29	22.740881215655907	26.778452905121757	28.029570927212987	22.45109495200935
30	23.40754511005568	26.866466352303654	27.44799151975611	22.27799701788456
31	23.314179982437086	26.622422492389674	26.98884988229015	23.074547642883086
32	22.355415584201914	26.852960591201587	26.77962810521765	24.011995719378852
33	21.87647634512047	26.05125716578258	27.740652503637243	24.3316139854597
34	23.122730846814836	26.517088403794414	28.081550931454558	22.278629817936192
35	23.26823869868828	26.193329817375716	27.995164684405438	22.54326679953057
36	22.067310760692557	26.887421074013563	27.80228722866664	23.242980936627244
37	22.357747904392227	26.450047758323898	28.27108358692042	22.921120750363453
38	22.432653350504513	25.621061610678623	28.293611268758678	23.65267377005818
39	22.16949892903111	25.485931679652023	28.61858121527623	23.725988176040637
40	22.850102504568365	25.422705914492806	28.863836435289052	22.86335514564978
41	21.24552045363447	26.035328684482824	29.43145808160698	23.28769278027573
42	22.16993284906652	26.046104365362115	29.11438909573415	22.669573689837215
43	21.049876757669946	25.02349948191756	29.039393249614488	24.88723051079801
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2988.0
1	3299.0
2	3610.0
3	6681.5
4	9753.0
5	9753.0
6	14329.0
7	18905.0
8	17802.0
9	16699.0
10	21420.0
11	26141.0
12	26141.0
13	42735.0
14	59329.0
15	86658.5
16	113988.0
17	99451.0
18	84914.0
19	84914.0
20	86485.5
21	88057.0
22	68102.5
23	48148.0
24	53971.5
25	59795.0
26	59795.0
27	66521.5
28	73248.0
29	80523.5
30	87799.0
31	95615.5
32	103432.0
33	103432.0
34	119452.5
35	135473.0
36	163003.5
37	190534.0
38	219508.0
39	248482.0
40	248482.0
41	272231.0
42	295980.0
43	313641.0
44	331302.0
45	369415.5
46	407529.0
47	407529.0
48	454342.0
49	501155.0
50	501953.0
51	502751.0
52	471682.0
53	440613.0
54	440613.0
55	419391.5
56	398170.0
57	378293.5
58	358417.0
59	330505.0
60	302593.0
61	302593.0
62	249586.0
63	196579.0
64	172665.0
65	148751.0
66	125589.0
67	102427.0
68	102427.0
69	84758.0
70	67089.0
71	57510.0
72	47931.0
73	34892.5
74	21854.0
75	21854.0
76	17223.5
77	12593.0
78	11211.5
79	9830.0
80	7751.5
81	5673.0
82	5673.0
83	4771.0
84	3869.0
85	3410.5
86	2952.0
87	1904.5
88	857.0
89	857.0
90	608.0
91	359.0
92	274.0
93	189.0
94	195.5
95	202.0
96	202.0
97	104.0
98	6.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5530973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.761603524666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05352580240472	34.232171311140505
2	10.022030567872081	8.571161953108582
3	3.388515113018615	4.346950194007225
4	1.7653343670611172	3.0195411317093876
5	0.9972499520963041	2.132200353326716
6	0.6795378459877779	1.7434876770080943
7	0.4929753463971062	1.4756291417047558
8	0.3627767018594565	1.2410330794320032
9	0.2756336991973607	1.0607885066803127
>10	1.7216720381375177	13.575135724246671
>50	0.1244544525365394	3.638662984514699
>100	0.09024259131015577	7.966247198396896
>500	0.013619771850030785	4.131525007753694
>1k	0.011837741725850833	9.22804091775779
>5k	4.242918181307241E-4	1.1064207276725069
>10k+	1.6971672725228965E-4	2.5310040915403675
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	51874	0.9378819965311709	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34603	0.6256222910507789	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32476	0.587166127912756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20547	0.37148979031356694	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	9130	0.16507041346974574	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	8184	0.14796673207408534	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7173	0.12968785058252863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.616000295065624E-5
2	0.0	0.0	0.0	0.0	3.616000295065624E-5
3	0.0	0.0	0.0	0.0	3.616000295065624E-5
4	0.0	0.0	0.0	1.808000147532812E-5	3.616000295065624E-5
5	0.0	0.0	0.0	1.808000147532812E-5	3.616000295065624E-5
6	1.808000147532812E-5	0.0	0.0	1.808000147532812E-5	1.0848000885196872E-4
7	1.808000147532812E-5	0.0	0.0	1.808000147532812E-5	1.0848000885196872E-4
8	1.808000147532812E-5	0.0	0.0	1.808000147532812E-5	1.808000147532812E-4
9	1.808000147532812E-5	0.0	0.0	3.616000295065624E-5	1.808000147532812E-4
10	1.808000147532812E-5	1.808000147532812E-5	0.0	3.616000295065624E-5	1.808000147532812E-4
11	1.808000147532812E-5	1.808000147532812E-5	0.0	7.232000590131248E-5	1.808000147532812E-4
12	1.808000147532812E-5	1.808000147532812E-5	0.0	2.3504001917926557E-4	1.9888001622860932E-4
13	1.808000147532812E-5	1.808000147532812E-5	0.0	3.2544002655590616E-4	2.1696001770393745E-4
14	1.808000147532812E-5	1.808000147532812E-5	0.0	5.062400413091873E-4	3.2544002655590616E-4
15	1.808000147532812E-5	1.808000147532812E-5	0.0	7.955200649144373E-4	3.616000295065624E-4
16	1.808000147532812E-5	1.808000147532812E-5	0.0	0.001663360135730187	4.1584003393254677E-4
17	1.808000147532812E-5	3.616000295065624E-5	0.0	0.0026396802153979054	4.1584003393254677E-4
18	1.808000147532812E-5	3.616000295065624E-5	0.0	0.003182080259657749	5.062400413091873E-4
19	1.808000147532812E-5	3.616000295065624E-5	0.0	0.0045380803703073585	5.243200427845155E-4
20	1.808000147532812E-5	3.616000295065624E-5	0.0	0.005622880458827045	5.243200427845155E-4
21	1.808000147532812E-5	3.616000295065624E-5	0.0	0.008768800715534139	5.424000442598436E-4
22	1.808000147532812E-5	3.616000295065624E-5	0.0	0.013776961124200028	5.604800457351718E-4
23	1.808000147532812E-5	3.616000295065624E-5	0.0	0.022437281830882196	6.689600545871405E-4
24	1.808000147532812E-5	3.616000295065624E-5	0.0	0.032236642630510036	6.870400560624686E-4
25	1.808000147532812E-5	3.616000295065624E-5	0.0	0.03673856299786674	6.870400560624686E-4
26	1.808000147532812E-5	3.616000295065624E-5	0.0	0.04666448380782188	6.870400560624686E-4
27	1.808000147532812E-5	3.616000295065624E-5	0.0	0.09488384774252198	6.870400560624686E-4
28	1.808000147532812E-5	3.616000295065624E-5	0.0	0.17029553389611557	6.870400560624686E-4
29	1.808000147532812E-5	3.616000295065624E-5	0.0	0.2632809814837281	6.870400560624686E-4
30	1.808000147532812E-5	3.616000295065624E-5	0.0	0.4067638731919321	6.870400560624686E-4
31	1.808000147532812E-5	3.616000295065624E-5	0.0	0.779031103568938	6.870400560624686E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1820	0.0	18.398352	5
CAAGACG	1965	0.0	17.229008	4
CTTATAC	5045	0.0	16.538157	37
TAATCCC	1270	0.0	15.586614	5
CGCTTCG	1295	0.0	15.571428	32
AGACGGA	2210	0.0	15.402715	6
TATACTG	545	0.0	15.275229	5
CGCAAGA	2175	0.0	15.14023	2
GACGGAC	2205	0.0	15.102041	7
ACGGACC	2255	0.0	15.013303	8
TACGGAT	2280	0.0	15.010964	27
ACGGATG	2290	0.0	14.86463	28
GTTACGG	1805	0.0	14.861496	25
GCGCAAG	2275	0.0	14.8	1
TACGTCG	75	0.004107036	14.8	12
CGGACCA	2275	0.0	14.718681	9
TCGTTAC	1505	0.0	14.504984	23
CGTTACG	1205	0.0	14.431535	24
ACTAGAC	335	0.0	14.358209	3
GTAAACG	1375	0.0	14.261818	27
>>END_MODULE