FastQCFastQC Report
Thu 9 Feb 2017
SRR2727227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5425028
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT378510.6977106846268812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT259460.47826481264244164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT235660.43439407133013874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139720.25754705782163706No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG100590.18541839784052727No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC99280.1830036637598921No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT94900.17492997271166158No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA76400.14082876622940932No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT65080.11996251447918795No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC61810.11393489581989255No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA61030.11249711522226245No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC58680.10816534034478716No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG56680.10447872342778691No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG56140.10348333686019685No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG55760.10278287964596682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG22400.018.582595
TTATGCG1002.879824E-718.54
CAAGACG22650.018.3774834
CTTATAC35850.016.77126937
GACGGAC24800.016.3366957
CGAACGA10650.016.1549316
ACGGACC25900.015.8571428
AGACGGA27100.015.5645756
CGCAAGA26150.015.56405452
CGGACCA26300.015.5456289
CTAGCGG13200.015.41666829
CGAGCCG20750.015.3349415
TATACTG7000.015.3285715
ACGAACG11300.015.22566415
TACGGAT24250.015.18144327
TATACCG1101.4537698E-515.1363645
ACGGATG24600.015.11585328
GCGCAAG26900.015.0613371
TCTAGCG13750.014.828
CGGATGG26150.014.785851529
Produced by FastQC (version 0.11.5)