##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727227.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5425028
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225650263924905	31.0	31.0	34.0	30.0	34.0
2	31.44100988971854	31.0	31.0	34.0	30.0	34.0
3	31.60390729780565	31.0	31.0	34.0	30.0	34.0
4	35.406935595539785	37.0	35.0	37.0	33.0	37.0
5	35.22880545501332	37.0	35.0	37.0	33.0	37.0
6	35.237242277827875	37.0	35.0	37.0	32.0	37.0
7	35.13184890474298	37.0	35.0	37.0	32.0	37.0
8	35.14628864588349	37.0	35.0	37.0	32.0	37.0
9	36.71093033989871	39.0	35.0	39.0	32.0	39.0
10	36.44675253289015	38.0	35.0	39.0	32.0	39.0
11	36.60120427028211	39.0	35.0	39.0	32.0	39.0
12	36.463388944720656	38.0	35.0	39.0	32.0	39.0
13	36.52479452640613	38.0	35.0	39.0	32.0	39.0
14	37.53242084649148	39.0	36.0	41.0	32.0	41.0
15	37.53130619786663	39.0	36.0	41.0	32.0	41.0
16	37.46985324315377	39.0	36.0	41.0	32.0	41.0
17	37.46618690262981	39.0	36.0	41.0	32.0	41.0
18	37.39174876148105	39.0	36.0	41.0	32.0	41.0
19	37.4019199532242	39.0	36.0	41.0	32.0	41.0
20	37.326468361084956	39.0	36.0	41.0	31.0	41.0
21	37.21909859267086	39.0	36.0	41.0	31.0	41.0
22	37.15743531646288	39.0	36.0	40.0	31.0	41.0
23	37.037570497332	39.0	36.0	40.0	31.0	41.0
24	36.90214262488599	39.0	36.0	40.0	31.0	41.0
25	36.770877680262664	39.0	35.0	40.0	30.0	41.0
26	36.54380051126003	38.0	35.0	40.0	30.0	41.0
27	36.35908662591235	38.0	35.0	40.0	30.0	41.0
28	36.10953325955184	38.0	35.0	40.0	29.0	41.0
29	36.04403866671287	38.0	35.0	40.0	29.0	41.0
30	35.9336960104169	38.0	34.0	40.0	29.0	41.0
31	36.050876972432214	38.0	35.0	40.0	29.0	41.0
32	35.980494478553844	38.0	35.0	40.0	29.0	41.0
33	35.95758049543707	38.0	35.0	40.0	29.0	41.0
34	35.9908723051752	38.0	35.0	40.0	29.0	41.0
35	35.7621039006619	38.0	34.0	40.0	27.0	41.0
36	35.69883104750796	38.0	34.0	40.0	27.0	41.0
37	35.60864552219822	38.0	34.0	40.0	27.0	41.0
38	35.484588282309325	38.0	34.0	40.0	27.0	41.0
39	35.39206470455083	38.0	34.0	40.0	26.0	41.0
40	35.18061676363698	38.0	34.0	40.0	25.0	41.0
41	35.077001814552844	38.0	33.0	40.0	25.0	41.0
42	34.84180634643729	38.0	33.0	40.0	24.0	41.0
43	34.4626763216706	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	3.0
13	10.0
14	14.0
15	50.0
16	147.0
17	300.0
18	567.0
19	1140.0
20	2258.0
21	4405.0
22	7710.0
23	13320.0
24	21340.0
25	33484.0
26	49282.0
27	70638.0
28	96736.0
29	128423.0
30	163263.0
31	203819.0
32	248688.0
33	304206.0
34	371902.0
35	450183.0
36	557939.0
37	723510.0
38	957228.0
39	1014459.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.391075585232	18.6334337813556	12.195255029098467	25.780235604313933
2	18.104127757497288	23.24721641989682	33.29499866175806	25.35365716084783
3	20.28186766962309	24.87332784273187	29.841689296350175	25.003115191294867
4	14.786283130704577	15.607698245981402	33.844857574928646	35.76116104838537
5	14.403280499197423	37.959085188131745	32.859738235452426	14.777896077218402
6	34.57163723394607	34.57106580832394	15.901226684912961	14.956070272817026
7	28.283337892449588	31.927282218635554	21.415649836277343	18.373730052637516
8	28.51601871916606	32.6091773166885	20.45100965377506	18.42379431037038
9	28.896625049677162	12.961444622958629	19.021634542715724	39.120295784648484
10	17.25823350589158	26.435605493649067	31.933973428339907	24.372187572119444
11	36.826703935905954	22.004052329315165	20.392244980118075	20.776998754660806
12	21.985029386023445	25.669747695311436	28.707151373227934	23.638071545437185
13	30.30876522664952	18.702299785365163	25.038009020414272	25.95092596757104
14	23.05796394046261	20.92252795745939	24.944018722115352	31.075489379962644
15	26.39872089139448	27.156210069330516	22.175056055010224	24.27001298426478
16	24.530970162734643	25.79197747919458	25.087925813470456	24.589126544600322
17	24.31924775319132	25.888566842419987	25.19695750879074	24.595227895597958
18	24.352445738528907	24.15622555312157	27.28544442535596	24.205884282993562
19	24.703725031465275	25.721784293094892	26.892008667973695	22.682482007466138
20	24.030051089137235	24.211782870060762	26.551623328027063	25.206542712774937
21	24.99756683283478	25.71350783811623	26.669337006186883	22.619588322862114
22	25.08847880600801	24.612499695854105	25.38438511285103	24.914636385286858
23	22.89376202297942	24.917677844243386	26.853262324176026	25.33529780860117
24	23.24386159850235	26.035091431786157	26.53090454095352	24.190142428757973
25	24.267358620084543	24.573605887379752	25.547130816652007	25.6119046758837
26	23.665794904653026	25.89577417849272	26.661595110661178	23.776835806193077
27	25.223630182185236	25.253399613790013	25.33480011531738	24.188170088707377
28	23.095622732269767	26.551475863350383	26.307034728668683	24.045866675711167
29	23.526330186682905	26.058188086771167	26.89879204310098	23.51668968344495
30	24.03777455157835	26.40675771627354	26.501614369547955	23.053853362600158
31	24.521292793327518	25.98814236534816	25.434080708892193	24.056484132432125
32	22.984895193167667	26.2180397962923	25.598688154236253	25.198376856303785
33	22.713136227130992	25.359150220054165	26.611162191236616	25.316551361578227
34	24.007581159028117	25.820769957316347	26.924414030674125	23.247234852981403
35	24.226086943698725	25.66416247068218	27.004542649365128	23.10520793625397
36	23.08308823475197	26.29053711796511	26.663641183050114	23.962733464232812
37	23.33206390824158	26.006004024311025	27.138882969820617	23.523049097626778
38	23.5098694421485	24.99288482935019	27.33449486343665	24.16275086506466
39	23.256580426865998	24.594674903060408	27.349702158219273	24.799042511854317
40	23.896982651518112	24.80593648548911	27.734843027538293	23.56223783545449
41	22.13763689330267	25.35857879443203	28.39375206911374	24.110032243151554
42	23.507159778714506	25.38195194568581	28.059136284642218	23.051751990957467
43	22.211811625672713	24.15677854565912	28.104666003567168	25.526743825100993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1465.0
1	1691.0
2	1917.0
3	4171.0
4	6425.0
5	6425.0
6	9354.0
7	12283.0
8	11863.5
9	11444.0
10	14427.0
11	17410.0
12	17410.0
13	29632.5
14	41855.0
15	62440.5
16	83026.0
17	74226.0
18	65426.0
19	65426.0
20	67444.0
21	69462.0
22	54404.0
23	39346.0
24	43313.0
25	47280.0
26	47280.0
27	53618.5
28	59957.0
29	66391.5
30	72826.0
31	79708.0
32	86590.0
33	86590.0
34	103688.5
35	120787.0
36	148877.5
37	176968.0
38	205859.0
39	234750.0
40	234750.0
41	256806.5
42	278863.0
43	294739.0
44	310615.0
45	350552.5
46	390490.0
47	390490.0
48	456791.0
49	523092.0
50	533218.5
51	543345.0
52	494418.0
53	445491.0
54	445491.0
55	424547.0
56	403603.0
57	389145.5
58	374688.0
59	348210.0
60	321732.0
61	321732.0
62	264266.5
63	206801.0
64	182375.5
65	157950.0
66	134815.5
67	111681.0
68	111681.0
69	93961.5
70	76242.0
71	66698.0
72	57154.0
73	42101.0
74	27048.0
75	27048.0
76	21182.5
77	15317.0
78	13727.0
79	12137.0
80	9918.5
81	7700.0
82	7700.0
83	6568.5
84	5437.0
85	4791.0
86	4145.0
87	2685.5
88	1226.0
89	1226.0
90	893.0
91	560.0
92	394.5
93	229.0
94	238.0
95	247.0
96	247.0
97	130.0
98	13.0
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5425028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.099059784129956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32665180910823	33.0134986496153
2	9.84245695264112	8.090314534386458
3	3.4992396797089924	4.314463823860789
4	1.6625800850682568	2.733219132484966
5	1.003617280097151	2.0623863297549354
6	0.6444752961084041	1.5892397234492497
7	0.48280451327435414	1.3889968088577311
8	0.3717447059539165	1.222268631554706
9	0.2745007713891772	1.0153551252702226
>10	1.639734384117741	12.4656459136822
>50	0.12437084237090575	3.523323468022261
>100	0.0953557174262299	8.190076470994018
>500	0.016459454262696415	4.707031677332051
>1k	0.015016431891546805	11.679526116141156
>5k	7.666046310999588E-4	1.9401473398331235
>10k+	2.2547195032351732E-4	2.0645062547607176
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37851	0.6977106846268812	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25946	0.47826481264244164	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23566	0.43439407133013874	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13972	0.25754705782163706	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	10059	0.18541839784052727	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	9928	0.1830036637598921	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	9490	0.17492997271166158	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	7640	0.14082876622940932	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	6508	0.11996251447918795	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	6181	0.11393489581989255	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6103	0.11249711522226245	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	5868	0.10816534034478716	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	5668	0.10447872342778691	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	5614	0.10348333686019685	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	5576	0.10278287964596682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.686616917000244E-5
2	0.0	0.0	0.0	0.0	3.686616917000244E-5
3	0.0	0.0	0.0	0.0	3.686616917000244E-5
4	0.0	0.0	0.0	0.0	3.686616917000244E-5
5	0.0	0.0	0.0	0.0	3.686616917000244E-5
6	0.0	0.0	0.0	0.0	9.21654229250061E-5
7	0.0	0.0	0.0	0.0	9.21654229250061E-5
8	0.0	0.0	0.0	0.0	1.1059850751000733E-4
9	0.0	1.843308458500122E-5	0.0	0.0	1.1059850751000733E-4
10	0.0	1.843308458500122E-5	0.0	1.843308458500122E-5	1.1059850751000733E-4
11	0.0	1.843308458500122E-5	0.0	1.843308458500122E-5	1.1059850751000733E-4
12	0.0	1.843308458500122E-5	0.0	5.5299253755003664E-5	1.1059850751000733E-4
13	0.0	1.843308458500122E-5	0.0	5.5299253755003664E-5	1.2903159209500854E-4
14	0.0	1.843308458500122E-5	0.0	1.2903159209500854E-4	2.764962687750183E-4
15	0.0	1.843308458500122E-5	0.0	2.580631841900171E-4	2.764962687750183E-4
16	1.843308458500122E-5	1.843308458500122E-5	0.0	7.926226371550524E-4	3.1336243794502077E-4
17	1.843308458500122E-5	1.843308458500122E-5	0.0	0.001493079851385099	3.1336243794502077E-4
18	1.843308458500122E-5	1.843308458500122E-5	0.0	0.0017880092047451183	3.31795522530022E-4
19	1.843308458500122E-5	1.843308458500122E-5	0.0	0.002506899503560166	3.31795522530022E-4
20	1.843308458500122E-5	1.843308458500122E-5	0.0	0.0035575853249052354	3.502286071150232E-4
21	1.843308458500122E-5	1.843308458500122E-5	0.0	0.0061197840822204055	3.686616917000244E-4
22	1.843308458500122E-5	1.843308458500122E-5	0.0	0.009548337815030632	3.686616917000244E-4
23	1.843308458500122E-5	1.843308458500122E-5	0.0	0.01452527065298096	5.345594529650354E-4
24	1.843308458500122E-5	1.843308458500122E-5	0.0	0.020921551003976385	5.345594529650354E-4
25	1.843308458500122E-5	1.843308458500122E-5	0.0	0.024423837075126617	5.529925375500366E-4
26	1.843308458500122E-5	1.843308458500122E-5	0.0	0.03225789802375213	5.529925375500366E-4
27	1.843308458500122E-5	1.843308458500122E-5	0.0	0.06390750425619923	5.529925375500366E-4
28	1.843308458500122E-5	1.843308458500122E-5	0.0	0.11762151273689278	5.714256221350378E-4
29	1.843308458500122E-5	1.843308458500122E-5	0.0	0.18200827719230206	5.714256221350378E-4
30	1.843308458500122E-5	1.843308458500122E-5	0.0	0.27697552897422834	5.714256221350378E-4
31	1.843308458500122E-5	1.843308458500122E-5	0.0	0.5666883193966925	6.082917913050402E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	2240	0.0	18.58259	5
TTATGCG	100	2.879824E-7	18.5	4
CAAGACG	2265	0.0	18.377483	4
CTTATAC	3585	0.0	16.771269	37
GACGGAC	2480	0.0	16.336695	7
CGAACGA	1065	0.0	16.15493	16
ACGGACC	2590	0.0	15.857142	8
AGACGGA	2710	0.0	15.564575	6
CGCAAGA	2615	0.0	15.5640545	2
CGGACCA	2630	0.0	15.545628	9
CTAGCGG	1320	0.0	15.416668	29
CGAGCCG	2075	0.0	15.33494	15
TATACTG	700	0.0	15.328571	5
ACGAACG	1130	0.0	15.225664	15
TACGGAT	2425	0.0	15.181443	27
TATACCG	110	1.4537698E-5	15.136364	5
ACGGATG	2460	0.0	15.115853	28
GCGCAAG	2690	0.0	15.061337	1
TCTAGCG	1375	0.0	14.8	28
CGGATGG	2615	0.0	14.7858515	29
>>END_MODULE