##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727226.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4674439
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.134244130686056	31.0	31.0	33.0	30.0	34.0
2	31.35485391936872	31.0	31.0	34.0	30.0	34.0
3	31.505703893023313	31.0	31.0	34.0	30.0	34.0
4	35.32953665669827	37.0	35.0	37.0	33.0	37.0
5	35.15233164878181	37.0	35.0	37.0	32.0	37.0
6	35.16198906435617	37.0	35.0	37.0	32.0	37.0
7	35.05800653297647	37.0	35.0	37.0	32.0	37.0
8	35.075450337462954	37.0	35.0	37.0	32.0	37.0
9	36.6106431167462	39.0	35.0	39.0	32.0	39.0
10	36.34793950675151	38.0	35.0	39.0	32.0	39.0
11	36.50423334222567	38.0	35.0	39.0	32.0	39.0
12	36.361778600597845	38.0	35.0	39.0	32.0	39.0
13	36.42524332866468	38.0	35.0	39.0	32.0	39.0
14	37.39254528725265	39.0	36.0	41.0	32.0	41.0
15	37.387032968020335	39.0	36.0	41.0	32.0	41.0
16	37.34038908198396	39.0	36.0	41.0	32.0	41.0
17	37.34960473331666	39.0	36.0	41.0	32.0	41.0
18	37.29666233744841	39.0	36.0	41.0	32.0	41.0
19	37.31097870781927	39.0	36.0	41.0	31.0	41.0
20	37.25175470254292	39.0	36.0	40.0	31.0	41.0
21	37.15367576729528	39.0	36.0	40.0	31.0	41.0
22	37.08773245302805	39.0	36.0	40.0	31.0	41.0
23	36.966950258629964	39.0	36.0	40.0	31.0	41.0
24	36.82266278370517	39.0	35.0	40.0	30.0	41.0
25	36.69969636998151	39.0	35.0	40.0	30.0	41.0
26	36.45182341667096	38.0	35.0	40.0	30.0	41.0
27	36.26725495829553	38.0	35.0	40.0	30.0	41.0
28	36.04253259054188	38.0	35.0	40.0	29.0	41.0
29	35.9651119631682	38.0	34.0	40.0	29.0	41.0
30	35.84665133078001	38.0	34.0	40.0	29.0	41.0
31	35.934889299015346	38.0	34.0	40.0	29.0	41.0
32	35.87207170742842	38.0	34.0	40.0	29.0	41.0
33	35.81159707079288	38.0	34.0	40.0	29.0	41.0
34	35.83602631246231	38.0	34.0	40.0	29.0	41.0
35	35.59765268088855	38.0	34.0	40.0	27.0	41.0
36	35.52728530632232	38.0	34.0	40.0	27.0	41.0
37	35.40986052016081	38.0	34.0	40.0	26.0	41.0
38	35.27865055036551	38.0	34.0	40.0	26.0	41.0
39	35.17606754521773	38.0	33.0	40.0	26.0	41.0
40	34.919888996305225	38.0	33.0	40.0	25.0	41.0
41	34.80160592533136	38.0	33.0	40.0	24.0	41.0
42	34.534963446950535	38.0	33.0	40.0	24.0	41.0
43	34.15126884744886	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	1.0
14	11.0
15	46.0
16	120.0
17	233.0
18	435.0
19	921.0
20	2039.0
21	4000.0
22	7166.0
23	12402.0
24	20187.0
25	30990.0
26	45337.0
27	64777.0
28	88466.0
29	116609.0
30	148059.0
31	182885.0
32	221910.0
33	268603.0
34	327699.0
35	393643.0
36	488342.0
37	631291.0
38	808613.0
39	809649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30562448242452	19.33592458902555	12.810328683292264	24.548122245257666
2	18.20490116567999	23.926315008068347	32.7813669191105	25.087416907141158
3	20.667806339969353	25.234172485725026	30.00890588154001	24.089115292765616
4	14.656047495752967	15.8432487834369	33.689368927479855	35.81133479333028
5	14.119576702145434	38.86825777382056	32.441390293038374	14.570775230995633
6	34.04797880558501	34.939764964309084	15.9499995614447	15.062256668661202
7	28.04351495441485	32.13324636389522	21.153640041083005	18.669598640606928
8	29.439211849807002	31.971109260383972	19.938948823591453	18.650730066217573
9	28.434791854166885	12.775287045140605	19.366174208284672	39.423746892407834
10	17.34218801443339	27.527538598749494	31.48067607685115	23.649597309965966
11	36.21080518967089	22.64896386496861	20.294028866351663	20.846202079008837
12	21.797139720937636	26.69128851611926	28.767473487192795	22.74409827575031
13	30.68607805129129	19.144072689792292	25.05603346198335	25.113815796933064
14	23.321044514646573	21.098275108521044	25.53793086186385	30.042749514968538
15	25.88684973747652	27.592145282032774	22.80658705782662	23.71441792266409
16	24.1386399523023	25.82928133194165	25.409808535313005	24.622270180443042
17	23.759578422137928	26.312098628305986	26.032835170166948	23.89548777938914
18	23.582509045470484	23.996826143201353	28.231751446537224	24.18891336479094
19	23.691570261158613	25.711256473771506	28.3339669209503	22.26320634411958
20	23.577588668929042	24.509679129409967	27.915200091390645	23.997532110270345
21	24.476605641874887	25.4024921493253	27.627122741359976	22.493779467439836
22	24.507497049378546	25.05682500081828	26.775769242041665	23.65990870776151
23	22.785686153996235	25.333307376564335	27.564954853405936	24.316051616033498
24	22.85831519033621	26.46396711990466	27.31502111804219	23.36269657171695
25	23.687484209335068	25.071479165735184	26.74481365571355	24.4962229692162
26	23.120464295287626	26.350712887685564	27.509739671434367	23.019083145592447
27	24.173146766916844	25.79918146327292	26.606508289016073	23.421163480794167
28	22.72803217669543	26.587211855797026	27.77220967050805	22.91254629699949
29	22.899860282699162	26.479562574246877	27.79910059795411	22.82147654509985
30	22.803891547199566	27.197723619882513	27.666528539574482	22.331856293343435
31	23.33933547961584	26.782935877438984	26.640437494210534	23.23729114873464
32	22.36022333375192	26.977141000235537	26.767447387804182	23.89518827820836
33	22.11150899605279	26.525514612555646	27.54161515424632	23.82136123714525
34	23.106623062147136	26.667692101661828	27.53648084829003	22.689203987901006
35	23.07481175815964	26.673404017038195	27.92765078333464	22.324133441467524
36	22.160990869706502	27.252361192433998	27.3227439699181	23.263903967941392
37	22.54935832941664	26.920770599423804	27.62962571551367	22.900245355645886
38	22.332476688646487	26.23914869784374	27.97360282164341	23.45477179186636
39	22.558814009552805	25.815290348210766	27.820087073550432	23.805808568685997
40	22.62134557751208	26.179355426394483	28.418426253931223	22.780872742162213
41	21.504612639078186	26.375165019802377	28.84977213308378	23.27045020803566
42	22.42885189003429	26.363997904347453	28.60212744245887	22.605022763159386
43	21.66022489543665	25.270176806243487	28.732517420807074	24.337080877512786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1254.0
1	1263.5
2	1273.0
3	2972.5
4	4672.0
5	4672.0
6	7774.0
7	10876.0
8	10421.5
9	9967.0
10	13352.0
11	16737.0
12	16737.0
13	31257.0
14	45777.0
15	75563.0
16	105349.0
17	90174.5
18	75000.0
19	75000.0
20	76928.5
21	78857.0
22	60625.0
23	42393.0
24	48289.0
25	54185.0
26	54185.0
27	60863.5
28	67542.0
29	74083.5
30	80625.0
31	86407.5
32	92190.0
33	92190.0
34	104419.5
35	116649.0
36	137528.0
37	158407.0
38	179636.5
39	200866.0
40	200866.0
41	222706.0
42	244546.0
43	258747.5
44	272949.0
45	301324.0
46	329699.0
47	329699.0
48	372489.0
49	415279.0
50	413686.5
51	412094.0
52	383984.5
53	355875.0
54	355875.0
55	343311.5
56	330748.0
57	317873.5
58	304999.0
59	285270.0
60	265541.0
61	265541.0
62	224304.5
63	183068.0
64	162230.0
65	141392.0
66	118584.5
67	95777.0
68	95777.0
69	79166.5
70	62556.0
71	53001.0
72	43446.0
73	32013.5
74	20581.0
75	20581.0
76	16160.5
77	11740.0
78	10351.0
79	8962.0
80	7158.5
81	5355.0
82	5355.0
83	4390.5
84	3426.0
85	2925.0
86	2424.0
87	1594.5
88	765.0
89	765.0
90	535.5
91	306.0
92	221.5
93	137.0
94	138.5
95	140.0
96	140.0
97	74.5
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4674439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.10830639163942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03297330494229	34.50085934663351
2	9.933359759753715	8.564206320436897
3	3.4326600337577187	4.439284814206891
4	1.705483385170212	2.940820012550715
5	1.0068218846784607	2.1701193143263464
6	0.699526106949574	1.8093231448399774
7	0.513563785746086	1.5497205519314774
8	0.35866608902262204	1.2369190126302567
9	0.29465983123302253	1.1432057657491708
>10	1.7854963421526961	14.271092970733894
>50	0.12667405152940117	3.7701734969259286
>100	0.08538520647467754	7.532969624381434
>500	0.013733481700102886	4.205451870597241
>1k	0.010548905975496695	8.255603720364366
>5k	2.487949522522802E-4	0.7042548726511739
>10k+	1.9903596180182416E-4	2.9059951610407664
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	53623	1.1471537012248956	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33351	0.7134759914505249	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31417	0.6721020426194458	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17085	0.36549840526317706	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	8627	0.18455690618703122	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6817	0.14583568210003384	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6333	0.13548149842152182	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	6020	0.12878550773686426	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5035	0.10771346037460323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1392941484528947E-5
2	0.0	0.0	0.0	0.0	2.1392941484528947E-5
3	0.0	0.0	0.0	0.0	2.1392941484528947E-5
4	0.0	0.0	0.0	0.0	4.278588296905789E-5
5	0.0	0.0	0.0	0.0	4.278588296905789E-5
6	0.0	0.0	0.0	0.0	1.0696470742264473E-4
7	0.0	0.0	0.0	0.0	1.0696470742264473E-4
8	0.0	0.0	0.0	0.0	1.4975059039170263E-4
9	0.0	0.0	0.0	2.1392941484528947E-5	1.4975059039170263E-4
10	0.0	0.0	0.0	8.557176593811579E-5	1.4975059039170263E-4
11	0.0	0.0	0.0	1.0696470742264473E-4	1.4975059039170263E-4
12	0.0	0.0	0.0	1.2835764890717369E-4	1.7114353187623157E-4
13	0.0	0.0	0.0	1.4975059039170263E-4	2.1392941484528946E-4
14	0.0	0.0	0.0	2.1392941484528946E-4	2.1392941484528946E-4
15	2.1392941484528947E-5	0.0	0.0	3.6368000523699206E-4	2.781082392988763E-4
16	4.278588296905789E-5	0.0	0.0	8.985035423502157E-4	3.4228706375246315E-4
17	4.278588296905789E-5	0.0	0.0	0.0014975059039170262	3.6368000523699206E-4
18	4.278588296905789E-5	0.0	0.0	0.0017542212017313736	3.8507294672152103E-4
19	4.278588296905789E-5	0.0	0.0	0.0024174023877517707	4.0646588820604995E-4
20	4.278588296905789E-5	0.0	0.0	0.0031019765152566973	4.278588296905789E-4
21	4.278588296905789E-5	0.0	0.0	0.004856197716988071	4.278588296905789E-4
22	4.278588296905789E-5	0.0	0.0	0.007637280109976834	4.278588296905789E-4
23	4.278588296905789E-5	0.0	0.0	0.01238651311954226	4.706447126596368E-4
24	4.278588296905789E-5	0.0	0.0	0.01790589202255073	4.706447126596368E-4
25	4.278588296905789E-5	0.0	0.0	0.020216329702879853	4.706447126596368E-4
26	4.278588296905789E-5	0.0	0.0	0.024430739175332054	4.920376541441658E-4
27	4.278588296905789E-5	0.0	0.0	0.04304259826687224	5.134305956286947E-4
28	4.278588296905789E-5	0.0	0.0	0.07622305050937664	5.134305956286947E-4
29	4.278588296905789E-5	0.0	0.0	0.12016415231859909	5.348235371132236E-4
30	4.278588296905789E-5	0.0	0.0	0.19576680752492437	5.348235371132236E-4
31	4.278588296905789E-5	0.0	0.0	0.4173976813046443	5.348235371132236E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1625	0.0	19.923077	5
CAAGACG	1770	0.0	19.231638	4
ACGGACC	1855	0.0	17.053907	8
GACGGAC	1920	0.0	16.572918	7
CGCAAGA	1970	0.0	16.52792	2
TAGGCCG	160	6.311893E-10	16.1875	5
CTTATAC	2345	0.0	16.093815	37
CGGACCA	1975	0.0	15.924051	9
AGACGGA	2055	0.0	15.754259	6
GCAAGAC	2290	0.0	15.591702	3
GCGCAAG	2140	0.0	15.387852	1
GTACTAG	185	3.0559022E-10	15.0	1
GCCGATA	100	1.0945037E-4	14.8	2
GAACAGT	3155	0.0	14.366085	1
CAGAGCG	2260	0.0	14.243363	14
AGCGAAA	2225	0.0	14.134831	17
GCGAAAG	2240	0.0	14.122768	18
GAGCGAA	2300	0.0	13.995651	16
AGAGCGA	2300	0.0	13.915216	15
TTAGAGT	935	0.0	13.850268	4
>>END_MODULE