FastQCFastQC Report
Thu 9 Feb 2017
SRR2727225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105073
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT397131.2789715410877618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT245870.7918332354827085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172670.5560899856460702No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167500.5394398134923076No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA51780.16675936443362202No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43800.1410594855579885No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT42030.13535913648406978No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41580.13390989519409044No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37590.12105995575627368No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.11925645548429939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG10900.017.9908285
CAAGACG11000.017.6590924
TAACGTG752.0686946E-417.2666665
CGGACCA12050.015.8132789
GACGGAC12200.015.7704937
ACGGACC12100.015.7479348
CTTATAC21400.015.733644537
CGCAAGA12600.015.4166662
TACCGTC6550.015.2519087
AGACGGA13750.015.0690916
GCGCAAG13100.014.8282441
AGAGCGA12900.014.48449615
TGTGCGA2301.8189894E-1214.47826110
TAGCCCG908.279603E-414.3888895
ACCGTCG7400.014.258
GTAAGAC2750.014.1272743
GAACAGT21900.014.10730651
CGTTATA1051.6570627E-414.0952392
GGACCGT1051.6570627E-414.0952396
GACCGTG4500.013.97777757
Produced by FastQC (version 0.11.5)