##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727225.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3105073
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60388113258529	31.0	30.0	31.0	27.0	34.0
2	30.826293938983078	31.0	31.0	33.0	27.0	34.0
3	30.931835096952632	31.0	31.0	34.0	27.0	34.0
4	34.78966967926358	35.0	35.0	37.0	32.0	37.0
5	34.575911741849545	35.0	35.0	37.0	30.0	37.0
6	34.65353149507274	35.0	35.0	37.0	31.0	37.0
7	34.575540414025696	35.0	35.0	37.0	30.0	37.0
8	34.619024415851094	35.0	35.0	37.0	30.0	37.0
9	36.066818074808545	37.0	35.0	39.0	31.0	39.0
10	35.759414029879494	37.0	35.0	39.0	30.0	39.0
11	35.97383314337537	37.0	35.0	39.0	30.0	39.0
12	35.79354237404402	37.0	35.0	39.0	30.0	39.0
13	35.914858684481814	37.0	35.0	39.0	30.0	39.0
14	36.725105979795	38.0	36.0	40.0	31.0	41.0
15	36.7687091414598	38.0	36.0	40.0	31.0	41.0
16	36.6770977687159	38.0	36.0	40.0	31.0	41.0
17	36.707348265242075	38.0	36.0	40.0	31.0	41.0
18	36.649645596093876	38.0	36.0	40.0	30.0	41.0
19	36.68480161335982	38.0	35.0	40.0	30.0	41.0
20	36.597184349611105	38.0	35.0	40.0	30.0	41.0
21	36.494009319587654	38.0	35.0	40.0	30.0	41.0
22	36.417062014323015	38.0	35.0	40.0	30.0	41.0
23	36.276689147082855	38.0	35.0	40.0	30.0	41.0
24	36.117931204838015	38.0	34.0	40.0	29.0	41.0
25	35.985824165808665	38.0	34.0	40.0	29.0	41.0
26	35.670787128032096	38.0	34.0	40.0	27.0	41.0
27	35.466486295169226	38.0	34.0	40.0	27.0	41.0
28	35.21612374330652	38.0	33.0	40.0	27.0	41.0
29	35.150502097696254	38.0	33.0	40.0	27.0	41.0
30	35.032044335189546	37.0	33.0	40.0	26.0	41.0
31	35.07031171247826	37.0	33.0	40.0	26.0	41.0
32	35.00326626781399	37.0	33.0	40.0	26.0	41.0
33	34.910748636183435	37.0	33.0	40.0	25.0	41.0
34	34.946523962560626	37.0	33.0	40.0	25.0	41.0
35	34.6733867448527	37.0	33.0	40.0	25.0	41.0
36	34.57128544159831	37.0	33.0	40.0	24.0	41.0
37	34.43905376781802	37.0	33.0	40.0	24.0	41.0
38	34.30435194277236	37.0	33.0	40.0	24.0	41.0
39	34.16863919141353	37.0	33.0	40.0	23.0	41.0
40	33.89035716712618	37.0	32.0	40.0	22.0	41.0
41	33.75208473359564	37.0	32.0	40.0	22.0	41.0
42	33.44995785928382	37.0	31.0	40.0	20.0	41.0
43	33.093400380602965	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	6.0
14	25.0
15	60.0
16	116.0
17	239.0
18	529.0
19	1194.0
20	2398.0
21	4682.0
22	8051.0
23	13330.0
24	20753.0
25	30779.0
26	43701.0
27	58884.0
28	78243.0
29	98673.0
30	120928.0
31	145589.0
32	172880.0
33	204209.0
34	241204.0
35	286592.0
36	347910.0
37	431507.0
38	514969.0
39	277618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18446587246097	22.559308589524303	14.801262321368934	22.454963216645794
2	20.000882426918785	26.06312315362634	30.927388824674974	23.008605594779898
3	23.148924357011897	25.68090991741579	30.05826272039337	21.111903005178945
4	17.19772771847876	18.038384282752773	32.52754444098416	32.23634355778431
5	13.909238204705654	40.32156409849301	32.2300957175564	13.539101979244933
6	33.42726563916533	35.91570955014584	15.413325226170205	15.243699584518625
7	26.556766942355303	33.45141966066498	21.558494760026576	18.433318636953143
8	28.696813247224785	33.63418509001237	20.194694295432022	17.474307367330816
9	27.973609638163094	13.068162970725647	20.1190439000951	38.83918349101616
10	17.179274046053024	28.839611822330745	32.185265853653036	21.79584827796319
11	35.555621397628975	22.923841081997107	20.460388531928235	21.060148988445683
12	20.422611642302773	27.907846288960037	29.650446221393185	22.01909584734401
13	30.595931238975705	19.89293005349633	25.766479564248566	23.7446591432794
14	22.327268956317614	21.900612320547697	26.953794645085637	28.818324078049052
15	25.530671903687935	28.524321328355246	23.444827223063676	22.500179544893147
16	23.017107810347774	26.581661687180947	26.711578117487093	23.689652384984186
17	22.568358296246174	27.451399693340544	27.14358084334893	22.83666116706435
18	22.132716364478387	25.20530113140657	29.328779065741774	23.333203438373268
19	22.901522766131425	26.655669609055888	29.500884520267316	20.941923104545367
20	22.575024806180082	25.143627863177453	29.407682202640643	22.87366512800182
21	23.368436104400768	26.41219707233936	29.12079683794874	21.098569985311133
22	23.662599880904573	26.03210939002078	27.964978601147216	22.34031212792743
23	22.11510003146464	26.264567692933465	28.940446810751308	22.679885464850585
24	21.831564024420683	27.190149796800267	28.815747649089086	22.16253852968996
25	22.731091990429857	26.068759091976258	28.097890130119325	23.10225878747456
26	21.974201572716648	27.055724615814185	28.928949496517475	22.041124314951695
27	23.225734145380798	26.76632723288631	27.92762038122775	22.080318240505132
28	21.945152336193065	27.5314622232714	28.722223277842424	21.801162162693117
29	22.241763720208834	27.30840788606258	29.068302097889486	21.381526295839098
30	22.484559944323372	27.593103286138522	28.541647813111	21.38068895642711
31	22.42146964016627	27.51201018462368	28.27392463880881	21.792595536401237
32	21.7600359154197	27.61094505668627	28.204328851527805	22.424690176366223
33	21.37943294730913	27.180617009648405	28.826021159566938	22.613928883475527
34	22.194776097051502	27.463058034384375	29.031716806658007	21.310449061906116
35	22.09764472526089	27.30850450214858	29.150908851418304	21.442941921172224
36	21.359530033593412	27.77541784041792	28.702739033832696	22.162313092155966
37	21.41585721173061	27.627208764496036	29.06572566892952	21.89120835484383
38	21.7529185304178	26.553514200793348	29.003955784614405	22.689611484174446
39	21.48941425853756	26.572644185821076	29.357023168215367	22.580918387425996
40	21.830436836750696	26.467912348598567	29.714921356116264	21.98672945853447
41	20.682830967259065	26.7318997009088	30.111240540882612	22.47402879094952
42	21.101114208909095	26.84358789632321	29.73846347573793	22.316834419029764
43	20.44689448525043	25.888344654054833	29.933821201627143	23.730939659067595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5135.0
1	4107.5
2	3080.0
3	3755.0
4	4430.0
5	4430.0
6	7375.5
7	10321.0
8	10008.5
9	9696.0
10	13238.5
11	16781.0
12	16781.0
13	30897.0
14	45013.0
15	65979.0
16	86945.0
17	71151.0
18	55357.0
19	55357.0
20	59921.0
21	64485.0
22	50516.5
23	36548.0
24	39613.5
25	42679.0
26	42679.0
27	47809.0
28	52939.0
29	56948.0
30	60957.0
31	66289.0
32	71621.0
33	71621.0
34	79653.0
35	87685.0
36	100054.0
37	112423.0
38	124549.0
39	136675.0
40	136675.0
41	151177.0
42	165679.0
43	175994.0
44	186309.0
45	206212.5
46	226116.0
47	226116.0
48	251935.5
49	277755.0
50	272879.0
51	268003.0
52	252888.5
53	237774.0
54	237774.0
55	222697.5
56	207621.0
57	196028.0
58	184435.0
59	166441.5
60	148448.0
61	148448.0
62	124555.5
63	100663.0
64	88054.0
65	75445.0
66	62426.5
67	49408.0
68	49408.0
69	39801.5
70	30195.0
71	25305.0
72	20415.0
73	15060.5
74	9706.0
75	9706.0
76	7418.5
77	5131.0
78	4468.5
79	3806.0
80	2989.5
81	2173.0
82	2173.0
83	1771.5
84	1370.0
85	1263.0
86	1156.0
87	769.0
88	382.0
89	382.0
90	267.0
91	152.0
92	123.5
93	95.0
94	76.5
95	58.0
96	58.0
97	31.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3105073.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.559980381413595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.75013179165464	41.83845039945447
2	8.783828650230664	8.882204084587219
3	3.007876855626471	4.562345844305471
4	1.466741847621344	2.9663375616133396
5	0.8714249673590687	2.2029614626774245
6	0.5809341991809497	1.762321302808862
7	0.4261892488597309	1.5083684042782173
8	0.3033475119455651	1.2269795402174717
9	0.23359044006607227	1.0629295260323743
>10	1.3928876650008755	12.750256201157683
>50	0.09649194725113597	3.3914621309341233
>100	0.0726135050003629	7.607359303100876
>500	0.009464783440767205	3.283555368043782
>1k	0.004156830565093174	3.6080890574028883
>5k	6.395123946297191E-5	0.16742407530520173
>10k+	2.5580495785188764E-4	3.1789557380806333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39713	1.2789715410877618	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24587	0.7918332354827085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17267	0.5560899856460702	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16750	0.5394398134923076	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	5178	0.16675936443362202	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4380	0.1410594855579885	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4203	0.13535913648406978	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4158	0.13390989519409044	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3759	0.12105995575627368	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3703	0.11925645548429939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.220536199954075E-5
2	0.0	0.0	0.0	0.0	3.220536199954075E-5
3	0.0	0.0	0.0	0.0	3.220536199954075E-5
4	0.0	0.0	0.0	0.0	3.220536199954075E-5
5	0.0	0.0	0.0	0.0	3.220536199954075E-5
6	0.0	0.0	0.0	0.0	6.44107239990815E-5
7	0.0	0.0	0.0	0.0	6.44107239990815E-5
8	0.0	0.0	0.0	0.0	6.44107239990815E-5
9	0.0	0.0	0.0	3.220536199954075E-5	9.661608599862226E-5
10	0.0	0.0	0.0	6.44107239990815E-5	9.661608599862226E-5
11	0.0	0.0	0.0	9.661608599862226E-5	1.28821447998163E-4
12	0.0	0.0	0.0	1.28821447998163E-4	1.932321719972445E-4
13	0.0	0.0	0.0	2.2543753399678526E-4	1.932321719972445E-4
14	0.0	0.0	0.0	3.2205361999540754E-4	2.8984825799586674E-4
15	0.0	0.0	0.0	6.763126019903558E-4	2.8984825799586674E-4
16	0.0	0.0	0.0	0.0014492412899793337	2.8984825799586674E-4
17	0.0	0.0	0.0	0.0022865807019673934	2.8984825799586674E-4
18	0.0	0.0	0.0	0.0026408396839623415	2.8984825799586674E-4
19	0.0	0.0	0.0	0.0036714112679476457	3.2205361999540754E-4
20	0.0	0.0	0.0	0.00473418821393249	3.2205361999540754E-4
21	0.0	0.0	0.0	0.00640886703790861	3.2205361999540754E-4
22	0.0	0.0	0.0	0.010209099753854417	3.2205361999540754E-4
23	0.0	0.0	0.0	0.015361957673780938	3.86464343994489E-4
24	0.0	0.0	0.0	0.022350521227681282	3.86464343994489E-4
25	0.0	0.0	0.0	0.02537782525563811	3.86464343994489E-4
26	0.0	0.0	0.0	0.03191551374154489	4.1866970599402977E-4
27	0.0	0.0	0.0	0.060288437663140286	4.1866970599402977E-4
28	0.0	0.0	0.0	0.11004572195243074	4.1866970599402977E-4
29	0.0	0.0	0.0	0.1766142052054815	4.1866970599402977E-4
30	0.0	0.0	0.0	0.28562935557392694	4.1866970599402977E-4
31	0.0	0.0	0.0	0.5804050339557234	4.1866970599402977E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1090	0.0	17.990828	5
CAAGACG	1100	0.0	17.659092	4
TAACGTG	75	2.0686946E-4	17.266666	5
CGGACCA	1205	0.0	15.813278	9
GACGGAC	1220	0.0	15.770493	7
ACGGACC	1210	0.0	15.747934	8
CTTATAC	2140	0.0	15.7336445	37
CGCAAGA	1260	0.0	15.416666	2
TACCGTC	655	0.0	15.251908	7
AGACGGA	1375	0.0	15.069091	6
GCGCAAG	1310	0.0	14.828244	1
AGAGCGA	1290	0.0	14.484496	15
TGTGCGA	230	1.8189894E-12	14.478261	10
TAGCCCG	90	8.279603E-4	14.388889	5
ACCGTCG	740	0.0	14.25	8
GTAAGAC	275	0.0	14.127274	3
GAACAGT	2190	0.0	14.1073065	1
CGTTATA	105	1.6570627E-4	14.095239	2
GGACCGT	105	1.6570627E-4	14.095239	6
GACCGTG	450	0.0	13.9777775	7
>>END_MODULE