FastQCFastQC Report
Thu 9 Feb 2017
SRR2727224.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727224.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551911
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT261681.686179168779653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178771.1519346148071636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111150.7162137519484042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80210.5168466490668602No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA37510.2417020048185753No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31850.20523084120158955No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.1351237281003872No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20480.13196633054343967No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19320.12449167510250267No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18180.11714589303123697No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15920.10258320225837693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG705.103824E-621.1428575
GCGTCGG450.003826402120.55555729
CCGTTGC450.003826402120.5555571
CGTTACA609.238805E-418.524
CGTACGC500.007035963718.53
CGGACCG701.21954654E-418.521
CGAGCGT650.001580271317.07692316
TAATACC1352.2208042E-816.4444454
AAGACGG3850.016.3376645
TCGTTAC1251.6587E-716.27999923
TACGCTG803.38352E-416.18755
CGACGTC700.002593042315.85714236
ATACCGT3200.015.6093766
GTTACAC2150.015.4883723
AACCGGG855.365653E-415.2352947
CGATAAC2450.015.1020410
CAAGACG4700.014.9574464
CCGATAA2600.014.9423079
TAAGACT1752.239176E-914.84
CGAACGA2500.014.79999916
Produced by FastQC (version 0.11.5)