##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1551911
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.336889808758364	31.0	30.0	31.0	27.0	34.0
2	30.525037196076322	31.0	30.0	31.0	27.0	34.0
3	30.531870062136296	31.0	30.0	33.0	27.0	34.0
4	34.36874795010797	35.0	35.0	37.0	30.0	37.0
5	34.12183108438564	35.0	33.0	37.0	30.0	37.0
6	34.32518166312372	35.0	35.0	37.0	30.0	37.0
7	34.256440607741034	35.0	35.0	37.0	30.0	37.0
8	34.346084279317566	35.0	35.0	37.0	30.0	37.0
9	35.737178871726535	37.0	35.0	39.0	30.0	39.0
10	35.389903802473206	37.0	34.0	39.0	30.0	39.0
11	35.65801518257168	37.0	35.0	39.0	30.0	39.0
12	35.44968171499526	37.0	35.0	39.0	30.0	39.0
13	35.61826483606341	37.0	35.0	39.0	30.0	39.0
14	36.31491754359625	38.0	35.0	40.0	30.0	41.0
15	36.413321382476184	38.0	35.0	40.0	30.0	41.0
16	36.277615791111735	38.0	35.0	40.0	30.0	41.0
17	36.35140223891705	38.0	35.0	40.0	30.0	41.0
18	36.31100043752509	38.0	35.0	40.0	30.0	41.0
19	36.35302475464121	38.0	35.0	40.0	30.0	41.0
20	36.27430761171227	38.0	34.0	40.0	30.0	41.0
21	36.171449909176495	38.0	34.0	40.0	29.0	41.0
22	36.09705131286523	38.0	34.0	40.0	29.0	41.0
23	35.935971199379345	38.0	34.0	40.0	29.0	41.0
24	35.77270088297589	38.0	34.0	40.0	27.0	41.0
25	35.642361578724554	38.0	34.0	40.0	27.0	41.0
26	35.28800942837572	38.0	34.0	40.0	27.0	41.0
27	35.06581176369006	38.0	33.0	40.0	26.0	41.0
28	34.8344917975322	37.0	33.0	40.0	25.0	41.0
29	34.74025314596005	37.0	33.0	40.0	25.0	41.0
30	34.58577972577036	37.0	33.0	40.0	25.0	41.0
31	34.58395938942375	37.0	33.0	39.0	25.0	40.0
32	34.48295101974276	37.0	33.0	39.0	25.0	40.0
33	34.330516376261265	37.0	33.0	39.0	25.0	41.0
34	34.34229733534977	37.0	33.0	40.0	25.0	41.0
35	34.030440534283215	37.0	33.0	40.0	24.0	41.0
36	33.888978169495545	36.0	32.0	40.0	23.0	41.0
37	33.71395137994382	36.0	32.0	39.0	22.0	41.0
38	33.54405052867078	36.0	32.0	39.0	21.0	41.0
39	33.36350731453028	36.0	31.0	39.0	21.0	41.0
40	33.0244666092321	36.0	31.0	39.0	19.0	40.0
41	32.80971267037865	36.0	31.0	39.0	18.0	40.0
42	32.440376413338136	35.0	30.0	39.0	16.0	40.0
43	32.03362048467986	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	11.0
14	20.0
15	52.0
16	111.0
17	223.0
18	454.0
19	887.0
20	1684.0
21	3181.0
22	5290.0
23	8541.0
24	12876.0
25	18711.0
26	25775.0
27	34443.0
28	44152.0
29	56073.0
30	67479.0
31	80169.0
32	94080.0
33	110344.0
34	129577.0
35	153348.0
36	188736.0
37	223564.0
38	213827.0
39	78295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.82676132845247	24.171489215554242	14.657799319677482	20.343950136315804
2	22.800598745675494	27.76286784486997	27.742441415777066	21.69409199367747
3	26.371099889104464	25.512674373723748	29.019640945904758	19.096584791267023
4	20.27242541614822	18.78000735866941	30.044377544846324	30.90318968033605
5	14.006408872673756	41.77507601917894	31.255593909702295	12.962921198445013
6	33.92623674940122	35.975709947284344	14.29966022536086	15.798393077953568
7	26.144991562016124	33.92262829505043	21.644153562929834	18.288226580003624
8	29.480749862588766	33.59496775266108	19.932650777009762	16.99163160774039
9	27.540819028926272	12.514699618728137	20.39949455864415	39.54498679370144
10	17.79257960024769	29.431971292168175	31.739320102763624	21.03612900482051
11	35.50364679417827	22.526742835123923	20.186466878577445	21.78314349212036
12	19.950886358818256	29.493186142762053	29.723933911158568	20.831993587261124
13	31.12543180633425	19.52064261417053	26.522977155262122	22.830948424233092
14	22.17975128728387	21.994689128435844	27.678971281213936	28.14658830306635
15	25.628467096373438	28.780516408479613	23.498383605760896	22.092632889386053
16	22.605871084102116	26.308789614868378	27.26412790424193	23.82121139678757
17	22.108484313855627	28.294083874655186	27.615887766759823	21.98154404472937
18	21.641898278960582	24.7213918839418	29.9655714792923	23.67113835780531
19	22.41842476791517	26.74251294049723	30.401163468781395	20.437898822806204
20	22.273893283828777	25.483420118808358	30.504068854463952	21.738617742898917
21	23.062598306217303	26.572915586009767	29.822586475641966	20.541899632130967
22	23.220532620749516	26.75217844322258	29.10211990249441	20.925169033533493
23	21.97142748521017	26.495204944097956	29.960029924396437	21.57333764629544
24	21.59795245990266	27.811259795181552	29.588423562949163	21.00236418196662
25	21.929350330012483	27.105613659546197	29.11371850576483	21.851317504676494
26	21.48667030519147	27.63212581133841	29.810150195468683	21.071053688001438
27	22.054421935278505	27.32637374179318	29.17390236940134	21.445301953526975
28	21.40064733093586	27.804107323164796	30.14457658976578	20.650668756133566
29	21.60188309767764	27.668403664900886	29.885992173520258	20.843721063901217
30	21.33356874202193	28.4466055076612	29.723805037788892	20.496020712527972
31	21.79074702093097	28.33364799914428	29.187885130010677	20.687719849914075
32	21.012094121376805	28.418446676388015	29.20966472948513	21.35979447275005
33	20.715363187708576	28.23989262270839	29.822328728902626	21.222415460680413
34	21.287174328940257	28.348146253232304	29.616389084167842	20.748290333659597
35	21.10391639726763	28.487393929162174	29.957645767057517	20.45104390651268
36	20.69906070644515	28.88361510421667	29.119646680769712	21.29767750856847
37	20.880901031051394	28.82330236721049	29.331192317085193	20.96460428465292
38	20.925169033533493	28.137696040559028	29.530430546597064	21.406704379310412
39	21.03831985210492	28.041427633414546	29.15766432482275	21.762588189657784
40	20.942373628384615	28.339640610834	29.509617497395148	21.208368263386237
41	20.219200714473963	28.411551951110596	29.759309651133343	21.609937683282094
42	20.801515035333857	28.642493029561617	29.2271270710756	21.32886486402893
43	20.44137840378733	27.860424985711163	29.603372873831034	22.094823736670467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434.0
1	433.0
2	432.0
3	725.5
4	1019.0
5	1019.0
6	2664.0
7	4309.0
8	4558.5
9	4808.0
10	6703.5
11	8599.0
12	8599.0
13	18114.0
14	27629.0
15	42681.5
16	57734.0
17	45871.5
18	34009.0
19	34009.0
20	40051.5
21	46094.0
22	34989.5
23	23885.0
24	23636.5
25	23388.0
26	23388.0
27	26480.0
28	29572.0
29	31148.5
30	32725.0
31	35274.0
32	37823.0
33	37823.0
34	41629.0
35	45435.0
36	51227.0
37	57019.0
38	62811.0
39	68603.0
40	68603.0
41	75141.5
42	81680.0
43	86763.5
44	91847.0
45	99511.0
46	107175.0
47	107175.0
48	117257.0
49	127339.0
50	124120.5
51	120902.0
52	115077.5
53	109253.0
54	109253.0
55	103763.0
56	98273.0
57	93125.5
58	87978.0
59	79927.5
60	71877.0
61	71877.0
62	61186.5
63	50496.0
64	43750.5
65	37005.0
66	31118.5
67	25232.0
68	25232.0
69	20513.5
70	15795.0
71	13092.5
72	10390.0
73	7851.5
74	5313.0
75	5313.0
76	4157.5
77	3002.0
78	2502.0
79	2002.0
80	1579.5
81	1157.0
82	1157.0
83	900.0
84	643.0
85	631.0
86	619.0
87	415.0
88	211.0
89	211.0
90	149.5
91	88.0
92	77.5
93	67.0
94	53.0
95	39.0
96	39.0
97	22.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1551911.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.96165331663299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.200543789113	49.10125491020695
2	7.2641565943643736	8.275567391318337
3	2.3637603560268268	4.039310937708032
4	1.203013317966599	2.7410251021322307
5	0.6951454228786523	1.9798316291329017
6	0.442028662931859	1.5107210072363624
7	0.3291859715580034	1.3125684032010196
8	0.22589948654688186	1.0294086589671105
9	0.1690385909106908	0.8665845851328225
>10	0.9524487961450969	9.764505616068593
>50	0.07745457388816515	3.07726887674129
>100	0.0684549558279926	7.774351552638202
>500	0.00636783311726673	2.5319959862985195
>1k	0.0020468035019785918	1.9032557381851496
>5k	1.1371130566547733E-4	0.519534926353903
>10k+	3.4113391699643194E-4	3.57281467867863
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26168	1.686179168779653	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17877	1.1519346148071636	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11115	0.7162137519484042	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8021	0.5168466490668602	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3751	0.2417020048185753	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3185	0.20523084120158955	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2097	0.1351237281003872	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2048	0.13196633054343967	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1932	0.12449167510250267	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1818	0.11714589303123697	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1592	0.10258320225837693	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.288733696713278E-4
2	0.0	0.0	0.0	0.0	1.288733696713278E-4
3	0.0	0.0	0.0	0.0	1.288733696713278E-4
4	0.0	0.0	0.0	0.0	1.288733696713278E-4
5	0.0	0.0	0.0	0.0	1.288733696713278E-4
6	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
7	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
8	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
9	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
10	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
11	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
12	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
13	0.0	0.0	0.0	6.44366848356639E-5	1.288733696713278E-4
14	0.0	0.0	0.0	3.866201090139834E-4	1.933100545069917E-4
15	0.0	0.0	0.0	8.376769028636307E-4	1.933100545069917E-4
16	0.0	0.0	0.0	0.0023197206540839003	1.933100545069917E-4
17	0.0	0.0	0.0	0.003866201090139834	1.933100545069917E-4
18	0.0	0.0	0.0	0.004381694568825145	1.933100545069917E-4
19	0.0	0.0	0.0	0.00612148505938807	1.933100545069917E-4
20	0.0	0.0	0.0	0.008119022289293652	2.577467393426556E-4
21	0.0	0.0	0.0	0.013660577185160746	2.577467393426556E-4
22	0.0	0.0	0.0	0.02364826333468865	2.577467393426556E-4
23	0.0	0.0	0.0	0.03479580981125851	3.866201090139834E-4
24	0.0	0.0	0.0	0.05238702477139475	3.866201090139834E-4
25	0.0	0.0	0.0	0.06031273700618141	3.866201090139834E-4
26	0.0	0.0	0.0	0.07384444082167083	3.866201090139834E-4
27	0.0	0.0	0.0	0.09936136801659373	3.866201090139834E-4
28	0.0	0.0	0.0	0.14723782484949202	3.866201090139834E-4
29	0.0	0.0	0.0	0.19685407217295323	3.866201090139834E-4
30	0.0	0.0	0.0	0.2784309151749037	3.866201090139834E-4
31	0.0	0.0	0.0	0.4657483579921787	3.866201090139834E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	70	5.103824E-6	21.142857	5
GCGTCGG	45	0.0038264021	20.555557	29
CCGTTGC	45	0.0038264021	20.555557	1
CGTTACA	60	9.238805E-4	18.5	24
CGTACGC	50	0.0070359637	18.5	3
CGGACCG	70	1.21954654E-4	18.5	21
CGAGCGT	65	0.0015802713	17.076923	16
TAATACC	135	2.2208042E-8	16.444445	4
AAGACGG	385	0.0	16.337664	5
TCGTTAC	125	1.6587E-7	16.279999	23
TACGCTG	80	3.38352E-4	16.1875	5
CGACGTC	70	0.0025930423	15.857142	36
ATACCGT	320	0.0	15.609376	6
GTTACAC	215	0.0	15.488372	3
AACCGGG	85	5.365653E-4	15.235294	7
CGATAAC	245	0.0	15.10204	10
CAAGACG	470	0.0	14.957446	4
CCGATAA	260	0.0	14.942307	9
TAAGACT	175	2.239176E-9	14.8	4
CGAACGA	250	0.0	14.799999	16
>>END_MODULE