FastQCFastQC Report
Thu 9 Feb 2017
SRR2727223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587562
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211150.5885612569204379No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT143960.40127529503322873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130570.36395189825290825No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68400.1906587258979775No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG39490.11007475271507504No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39400.10982388597047243No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37390.1042211953410143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCC10400.016.8990385
AAGACGG10100.016.6683185
TATACCG1901.8189894E-1216.5526315
CTTATAC18350.016.23160737
ACGCGTA700.00259393115.8571436
TTAATCC12600.015.5634924
TAGTACT2450.015.102044
TCTTATA29650.014.91231137
ACGGATG22050.014.598639528
CCCTATA1406.004593E-714.5357152
CAAGACG12400.014.4717754
TACGGAT21800.014.42660527
TACTTAC13150.014.3498131
CCTATAC3100.014.3225813
GGGCGTC11700.014.07264911
TAATACC2405.456968E-1213.8749994
GTACCGT1203.304198E-513.8749996
GTTACGG17750.013.86197325
ATACCGG1356.5757977E-613.7037046
ACGGACC12450.013.6706838
Produced by FastQC (version 0.11.5)