##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727223.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3587562
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201864943379377	31.0	31.0	34.0	30.0	34.0
2	31.44297715272935	31.0	31.0	34.0	30.0	34.0
3	31.600124820142483	31.0	31.0	34.0	30.0	34.0
4	35.404463811357125	37.0	35.0	37.0	33.0	37.0
5	35.21988498038501	37.0	35.0	37.0	33.0	37.0
6	35.21785435345787	37.0	35.0	37.0	32.0	37.0
7	35.11699003390046	37.0	35.0	37.0	32.0	37.0
8	35.12520508356371	37.0	35.0	37.0	32.0	37.0
9	36.69396124722026	39.0	35.0	39.0	32.0	39.0
10	36.41095624270744	38.0	35.0	39.0	32.0	39.0
11	36.58084375963398	39.0	35.0	39.0	32.0	39.0
12	36.429294601737894	38.0	35.0	39.0	32.0	39.0
13	36.49571603222467	38.0	35.0	39.0	32.0	39.0
14	37.50362112208793	39.0	36.0	41.0	32.0	41.0
15	37.502343095394586	39.0	36.0	41.0	32.0	41.0
16	37.43983602234609	39.0	36.0	41.0	32.0	41.0
17	37.42001197470594	39.0	36.0	41.0	32.0	41.0
18	37.3496814828566	39.0	36.0	41.0	32.0	41.0
19	37.37566653900337	39.0	36.0	41.0	32.0	41.0
20	37.27927517350223	39.0	36.0	41.0	31.0	41.0
21	37.180844818849124	39.0	36.0	40.0	31.0	41.0
22	37.11622070921701	39.0	36.0	40.0	31.0	41.0
23	37.00804278783196	39.0	36.0	40.0	31.0	41.0
24	36.87518738352118	39.0	36.0	40.0	30.0	41.0
25	36.73437030495919	39.0	35.0	40.0	30.0	41.0
26	36.50848459204329	38.0	35.0	40.0	30.0	41.0
27	36.345354310253036	38.0	35.0	40.0	30.0	41.0
28	36.10106557043474	38.0	35.0	40.0	29.0	41.0
29	36.04911524873995	38.0	35.0	40.0	29.0	41.0
30	35.96257653526266	38.0	34.0	40.0	29.0	41.0
31	36.09217429552437	38.0	35.0	40.0	29.0	41.0
32	36.063757225659096	38.0	35.0	40.0	29.0	41.0
33	36.06444599424344	38.0	35.0	40.0	29.0	41.0
34	36.10795910983559	38.0	35.0	40.0	29.0	41.0
35	35.90097118879061	38.0	34.0	40.0	28.0	41.0
36	35.84320661217841	38.0	34.0	40.0	28.0	41.0
37	35.77680134865962	38.0	34.0	40.0	27.0	41.0
38	35.67555431794628	38.0	34.0	40.0	27.0	41.0
39	35.59502943781877	38.0	34.0	40.0	27.0	41.0
40	35.419276377662605	38.0	34.0	40.0	26.0	41.0
41	35.340500874967454	38.0	34.0	40.0	26.0	41.0
42	35.13553884225555	38.0	33.0	40.0	25.0	41.0
43	34.779491476384244	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	3.0
15	17.0
16	53.0
17	125.0
18	276.0
19	559.0
20	1212.0
21	2427.0
22	4546.0
23	8126.0
24	13665.0
25	21150.0
26	31457.0
27	45096.0
28	63092.0
29	83863.0
30	107481.0
31	134573.0
32	165332.0
33	201103.0
34	245426.0
35	298410.0
36	366722.0
37	469721.0
38	638640.0
39	684483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73183292720795	18.663677450034314	12.348748258566681	26.25574136419106
2	18.11631408739417	22.353815766807653	34.01647135296895	25.51339879282922
3	20.03065034137389	24.032755392101933	30.40410172702242	25.532492539501757
4	14.7127492152052	15.410409631945038	34.353664131797586	35.523177021052184
5	14.23359373301423	38.201932119918766	33.21247131060034	14.35200283646666
6	34.65835573015881	34.6830521674608	15.862555127967127	14.796036974413266
7	28.41155079689215	32.30547653253101	20.891820127429156	18.391152543147687
8	28.563938407196865	33.21860360880174	20.04288706369395	18.17457092030744
9	29.11208224415355	12.98656301967743	18.507192349567756	39.39416238660126
10	16.897519819866528	26.764861485320672	32.32574099067835	24.011877704134452
11	37.08000028989046	21.77559579458139	20.434517926101346	20.7098859894268
12	21.91649928279985	25.155495570529514	28.959583137517903	23.968422009152736
13	30.739454816390634	18.97982529639906	24.6530094810905	25.627710406119807
14	22.976578523242246	20.4975691012448	25.013226252257105	31.51262612325585
15	26.53885842251646	27.23091614862684	22.069137759849166	24.161087669007532
16	24.575240790263695	25.875984861028183	24.903653233031235	24.645121115676886
17	24.291844991110956	25.525691263314755	25.072570174396986	25.109893571177306
18	24.0485599970119	24.989226667023452	26.731050222964786	24.23116311299986
19	24.93816134745546	25.30523514297453	26.803216223162135	22.95338728640787
20	24.17354738398946	24.04449038093279	26.364255168273047	25.417707066804702
21	24.824295719488614	25.38916400608547	26.94188978476191	22.844650489664012
22	25.46827065288349	24.229992401525045	25.274991763208554	25.02674518238291
23	23.649012895108154	24.915806333103095	26.53278187248053	24.90239889930822
24	23.465740801134586	25.193153456302635	26.57554071539391	24.76556502716887
25	24.681413171396063	24.028908768684694	25.596798048368225	25.692880011551022
26	23.7723278371217	25.278336653136584	26.741001270500693	24.208334239241022
27	25.536450659249933	24.98256476125012	25.367282851139578	24.113701728360372
28	23.476583819317966	26.435250456995586	25.601146405274665	24.48701931841178
29	23.910416042984064	25.903357210272603	26.493590912157057	23.692635834586273
30	24.987526347976704	25.71473329241418	25.868514606855577	23.429225752753542
31	24.524955945012238	25.585676289357508	25.8546054395715	24.034762326058758
32	23.719227709514147	25.768781138834672	25.57212948514897	24.939861666502207
33	23.29885866780839	25.041852935224533	26.32941256485602	25.329875832111053
34	24.37621983954563	25.4340412792866	26.921764697028234	23.267974184139536
35	24.316931665571214	25.184512490655216	26.752680511166076	23.74587533260749
36	23.42314920271761	25.60964800050842	26.82941786093174	24.137784935842223
37	23.66361891446057	25.513426666911958	27.217871077907503	23.605083340719965
38	23.970596187605956	24.75717492826605	26.727259347713016	24.544969536414982
39	23.19413573897817	24.91168096885852	27.358523699381365	24.53565959278195
40	24.29613759985193	24.386226635247002	27.4520691210354	23.86556664386567
41	22.65513460115811	25.192456604234298	27.916256220798413	24.23615257380918
42	23.395191497735787	25.01821571306642	27.756704971231162	23.82988781796663
43	22.521255381788524	23.986317170267718	27.642337609775108	25.85008983816865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	507.0
1	625.0
2	743.0
3	1567.5
4	2392.0
5	2392.0
6	3676.0
7	4960.0
8	4760.5
9	4561.0
10	5884.0
11	7207.0
12	7207.0
13	12858.0
14	18509.0
15	29863.0
16	41217.0
17	36640.5
18	32064.0
19	32064.0
20	33450.5
21	34837.0
22	28878.0
23	22919.0
24	27103.5
25	31288.0
26	31288.0
27	36488.5
28	41689.0
29	47481.0
30	53273.0
31	59451.0
32	65629.0
33	65629.0
34	76230.5
35	86832.0
36	103261.0
37	119690.0
38	136485.5
39	153281.0
40	153281.0
41	170671.5
42	188062.0
43	201278.5
44	214495.0
45	240589.5
46	266684.0
47	266684.0
48	305074.5
49	343465.0
50	352575.5
51	361686.0
52	339929.5
53	318173.0
54	318173.0
55	301278.0
56	284383.0
57	268861.5
58	253340.0
59	230989.0
60	208638.0
61	208638.0
62	175206.0
63	141774.0
64	123861.5
65	105949.0
66	88152.0
67	70355.0
68	70355.0
69	57365.5
70	44376.0
71	36795.5
72	29215.0
73	21803.0
74	14391.0
75	14391.0
76	11224.0
77	8057.0
78	6802.0
79	5547.0
80	4407.0
81	3267.0
82	3267.0
83	2592.0
84	1917.0
85	1651.0
86	1385.0
87	917.0
88	449.0
89	449.0
90	318.5
91	188.0
92	136.0
93	84.0
94	82.5
95	81.0
96	81.0
97	41.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3587562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.745346703854715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92830934001226	37.36276531551822
2	9.915250098061914	9.269836069786672
3	3.414601905044714	4.788502497208738
4	1.749358138474857	3.270974107688683
5	1.0495296536405956	2.45303137677031
6	0.7074049294240519	1.984073321156591
7	0.5069916333557524	1.6589649774017767
8	0.40109755133916347	1.499955527953309
9	0.30575697946386615	1.2863444410945628
>10	1.8007117328172524	15.453992305469185
>50	0.12244733571239745	3.944151948366387
>100	0.08123074200319476	7.728450511053331
>500	0.012099003640090165	3.8897082548867568
>1k	0.004971372779554465	3.8579018208727183
>5k	5.9896057584993554E-5	0.19151055929551264
>10k+	1.7968817275498065E-4	1.3598369654772597
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21115	0.5885612569204379	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14396	0.40127529503322873	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13057	0.36395189825290825	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6840	0.1906587258979775	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	3949	0.11007475271507504	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3940	0.10982388597047243	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	3739	0.1042211953410143	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7874082733622444E-5
2	0.0	0.0	0.0	0.0	2.7874082733622444E-5
3	0.0	0.0	0.0	0.0	2.7874082733622444E-5
4	0.0	0.0	0.0	0.0	2.7874082733622444E-5
5	0.0	0.0	0.0	0.0	2.7874082733622444E-5
6	0.0	0.0	0.0	0.0	5.574816546724489E-5
7	0.0	0.0	0.0	0.0	5.574816546724489E-5
8	0.0	0.0	0.0	0.0	5.574816546724489E-5
9	0.0	0.0	0.0	0.0	5.574816546724489E-5
10	0.0	0.0	0.0	0.0	8.362224820086734E-5
11	0.0	0.0	0.0	0.0	8.362224820086734E-5
12	0.0	0.0	0.0	2.7874082733622444E-5	8.362224820086734E-5
13	0.0	0.0	0.0	2.7874082733622444E-5	8.362224820086734E-5
14	0.0	0.0	0.0	1.393704136681122E-4	1.951185791353571E-4
15	0.0	0.0	0.0	2.2299266186897955E-4	2.50866744602602E-4
16	0.0	0.0	0.0	7.52600233807806E-4	3.066149100698469E-4
17	0.0	0.0	0.0	0.00125433372301301	3.3448899280346935E-4
18	0.0	0.0	0.0	0.0015330745503492344	3.902371582707142E-4
19	0.0	0.0	0.0	0.0018675635431527036	3.902371582707142E-4
20	0.0	0.0	0.0	0.002369297032357908	3.902371582707142E-4
21	0.0	0.0	0.0	0.003930245665440765	3.902371582707142E-4
22	0.0	0.0	0.0	0.0063552908632659175	3.902371582707142E-4
23	0.0	0.0	0.0	0.010229788363239437	4.738594064715815E-4
24	0.0	0.0	0.0	0.014410900773282803	4.738594064715815E-4
25	0.0	0.0	0.0	0.016445708812837242	4.738594064715815E-4
26	0.0	0.0	0.0	0.020013591402740914	4.738594064715815E-4
27	0.0	0.0	0.0	0.03581819631270484	4.738594064715815E-4
28	0.0	0.0	0.0	0.0650859831830084	4.738594064715815E-4
29	0.0	0.0	0.0	0.10416544717554707	4.738594064715815E-4
30	0.0	0.0	0.0	0.16769048172547263	4.738594064715815E-4
31	0.0	0.0	0.0	0.396230086058443	4.738594064715815E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCCC	1040	0.0	16.899038	5
AAGACGG	1010	0.0	16.668318	5
TATACCG	190	1.8189894E-12	16.552631	5
CTTATAC	1835	0.0	16.231607	37
ACGCGTA	70	0.002593931	15.857143	6
TTAATCC	1260	0.0	15.563492	4
TAGTACT	245	0.0	15.10204	4
TCTTATA	2965	0.0	14.912311	37
ACGGATG	2205	0.0	14.5986395	28
CCCTATA	140	6.004593E-7	14.535715	2
CAAGACG	1240	0.0	14.471775	4
TACGGAT	2180	0.0	14.426605	27
TACTTAC	1315	0.0	14.34981	31
CCTATAC	310	0.0	14.322581	3
GGGCGTC	1170	0.0	14.072649	11
TAATACC	240	5.456968E-12	13.874999	4
GTACCGT	120	3.304198E-5	13.874999	6
GTTACGG	1775	0.0	13.861973	25
ATACCGG	135	6.5757977E-6	13.703704	6
ACGGACC	1245	0.0	13.670683	8
>>END_MODULE