##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727222.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2446817
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.618227272411463	31.0	30.0	31.0	27.0	34.0
2	30.853499873509133	31.0	31.0	33.0	27.0	34.0
3	30.957896319994507	31.0	31.0	34.0	27.0	34.0
4	34.82273664111374	35.0	35.0	37.0	32.0	37.0
5	34.598605044839886	35.0	35.0	37.0	31.0	37.0
6	34.67072813373456	35.0	35.0	37.0	31.0	37.0
7	34.600362838741106	35.0	35.0	37.0	30.0	37.0
8	34.63069408133097	35.0	35.0	37.0	30.0	37.0
9	36.109612610996244	38.0	35.0	39.0	31.0	39.0
10	35.78544124877341	37.0	35.0	39.0	30.0	39.0
11	35.99379643021934	37.0	35.0	39.0	30.0	39.0
12	35.80610769011332	37.0	35.0	39.0	30.0	39.0
13	35.920494667153285	37.0	35.0	39.0	30.0	39.0
14	36.76120568068638	38.0	36.0	40.0	31.0	41.0
15	36.78969330358584	38.0	36.0	40.0	31.0	41.0
16	36.70345555061944	38.0	36.0	40.0	31.0	41.0
17	36.70783307456177	38.0	36.0	40.0	31.0	41.0
18	36.6395958504457	38.0	35.0	40.0	30.0	41.0
19	36.66386370537723	38.0	35.0	40.0	30.0	41.0
20	36.5665446169452	38.0	35.0	40.0	30.0	41.0
21	36.45832974022986	38.0	35.0	40.0	30.0	41.0
22	36.37247084681854	38.0	35.0	40.0	30.0	41.0
23	36.23437592594787	38.0	34.0	40.0	29.0	41.0
24	36.08040813841002	38.0	34.0	40.0	29.0	41.0
25	35.94852782206434	38.0	34.0	40.0	28.0	41.0
26	35.64239867550373	38.0	34.0	40.0	27.0	41.0
27	35.444929064985246	38.0	34.0	40.0	27.0	41.0
28	35.19707440319403	38.0	33.0	40.0	26.0	41.0
29	35.12505144438673	38.0	33.0	40.0	26.0	41.0
30	35.018774187035646	38.0	33.0	40.0	26.0	41.0
31	35.119280273105836	38.0	33.0	40.0	26.0	41.0
32	35.07735846203455	38.0	33.0	40.0	26.0	41.0
33	35.016876211012104	38.0	33.0	40.0	26.0	41.0
34	35.06275990398955	38.0	33.0	40.0	26.0	41.0
35	34.80637579353094	38.0	33.0	40.0	25.0	41.0
36	34.719870754535386	37.0	33.0	40.0	25.0	41.0
37	34.60725751047177	37.0	33.0	40.0	24.0	41.0
38	34.48994183054965	37.0	33.0	40.0	24.0	41.0
39	34.37266007224897	37.0	33.0	40.0	24.0	41.0
40	34.117849843286194	37.0	33.0	40.0	23.0	41.0
41	33.999969347932435	37.0	32.0	40.0	23.0	41.0
42	33.73192355619566	37.0	32.0	40.0	22.0	41.0
43	33.364979072811735	36.0	31.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	5.0
14	8.0
15	38.0
16	92.0
17	183.0
18	366.0
19	829.0
20	1772.0
21	3354.0
22	6063.0
23	9924.0
24	15694.0
25	23620.0
26	33126.0
27	45572.0
28	59857.0
29	76223.0
30	94334.0
31	113550.0
32	135145.0
33	159993.0
34	189837.0
35	224965.0
36	274624.0
37	341311.0
38	418735.0
39	217594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.857782580389134	22.53119052221723	14.521314834742444	23.08971206265119
2	20.576855563779393	25.283255756356116	31.03730274883655	23.102585931027946
3	22.84155292365551	25.127011950628102	29.98892029931131	22.04251482640508
4	17.321524249668037	17.936731680383126	32.75124375872818	31.99050031122066
5	14.098765866021038	39.71915349615439	32.64788498690339	13.534195650921177
6	33.76823849106819	36.33250872460017	15.134192708322692	14.765060076008954
7	27.417170961293795	32.9782325363932	21.178984779000636	18.425611723312368
8	28.559185259870272	33.82422960115121	19.77066531743077	17.84591982154775
9	27.837022548069594	13.065954666818156	19.46357246986595	39.6334503152463
10	17.045819119288446	28.258222825818198	32.39384065093548	22.302117403957876
11	36.419683204751315	22.37584584380442	20.530141812812317	20.674329138631943
12	20.835313797476477	27.102639878666857	29.419077928590493	22.642968395266177
13	30.787958396561738	19.661911781714775	25.259510621350106	24.290619200373385
14	22.514188842075235	21.202035133808536	26.139960610049712	30.143815414066523
15	25.67409005250495	28.34911642350041	22.669410912217792	23.307382611776852
16	23.749303687198513	26.463278618711577	25.684716102593697	24.102701591496217
17	23.15583878974194	27.151070145417496	26.28725401204912	23.40583705279144
18	22.876087586443937	25.157091846263942	28.11967548043029	23.84714508686183
19	23.62689976406082	26.312470446298192	28.238809849694523	21.82181993994647
20	23.384421474920273	24.908769229574588	28.06683131595048	23.639977979554665
21	24.176716117306686	25.959889930468847	27.771794948294048	22.091599003930412
22	24.37199022239914	25.697794318087542	26.9154170499878	23.01479840952552
23	22.91720222640271	25.802256564344617	27.710122988355895	23.570418220896784
24	22.819279087892554	26.58466080626381	27.53360794861242	23.06245215723121
25	23.621586739016447	25.50452281474258	27.021309726064512	23.85258072017646
26	22.903797055521522	26.520741028037655	27.68523350949417	22.890228406946658
27	24.11038504309885	26.160027496948075	26.88926061900011	22.840326840952958
28	22.937105635607402	26.779730564239173	27.667332702036973	22.615831098116452
29	23.04083223224295	26.758601072331935	27.79366008982282	22.4069066056023
30	23.24468891625324	27.07059825070694	27.379979785983178	22.304733047056647
31	23.297206125345703	26.94619172582175	27.093076433586983	22.663525715245562
32	22.753969749270176	26.961272543063092	27.02376189146961	23.260995816197124
33	22.419494388015124	26.681603078611925	27.514848883263436	23.38405365010951
34	23.15040315642731	26.808339160631956	27.83739037288036	22.203867310060378
35	23.075244286761126	26.60930506858502	28.0503200688895	22.26513057576435
36	22.27882183260947	27.05727481867258	27.605824219792492	23.058079128925456
37	22.45697164928967	26.972675112196786	27.854310314175518	22.71604292433803
38	22.610804159036004	26.03370010916223	27.97107425688149	23.384421474920273
39	22.480798523142514	26.05274526047514	28.177914408801314	23.288541807581034
40	22.586119027291375	25.93614479546284	28.534336650431968	22.943399526813817
41	21.653887479120833	26.128149346681834	28.96346559632371	23.25449757787362
42	22.10553547731604	26.1900665231605	28.72090556833633	22.98349243118713
43	21.585472064318665	25.25869323288174	28.912746641861652	24.243088060937946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1296.0
1	1119.0
2	942.0
3	1272.5
4	1603.0
5	1603.0
6	3055.5
7	4508.0
8	4554.0
9	4600.0
10	6325.5
11	8051.0
12	8051.0
13	15736.0
14	23421.0
15	36793.0
16	50165.0
17	41326.0
18	32487.0
19	32487.0
20	36167.5
21	39848.0
22	32295.0
23	24742.0
24	27814.0
25	30886.0
26	30886.0
27	35060.5
28	39235.0
29	43133.5
30	47032.0
31	51637.5
32	56243.0
33	56243.0
34	63150.0
35	70057.0
36	80570.0
37	91083.0
38	101931.5
39	112780.0
40	112780.0
41	124470.0
42	136160.0
43	144923.5
44	153687.0
45	168165.0
46	182643.0
47	182643.0
48	200394.0
49	218145.0
50	215862.5
51	213580.0
52	204035.0
53	194490.0
54	194490.0
55	183262.5
56	172035.0
57	162177.0
58	152319.0
59	139095.0
60	125871.0
61	125871.0
62	106811.5
63	87752.0
64	76124.5
65	64497.0
66	53802.0
67	43107.0
68	43107.0
69	34879.5
70	26652.0
71	21766.0
72	16880.0
73	12661.0
74	8442.0
75	8442.0
76	6502.0
77	4562.0
78	3791.5
79	3021.0
80	2368.0
81	1715.0
82	1715.0
83	1351.5
84	988.0
85	872.5
86	757.0
87	523.5
88	290.0
89	290.0
90	208.0
91	126.0
92	92.0
93	58.0
94	54.0
95	50.0
96	50.0
97	29.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2446817.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.879250895326436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50382679791886	43.62740557071709
2	8.809365563878783	9.3166530376199
3	3.0594943404583987	4.853513065257928
4	1.5544867816624988	3.2880038616399916
5	0.8879498626972312	2.347706178601878
6	0.5940507372093391	1.8847774786467764
7	0.4182206622294318	1.548063672934779
8	0.33200932325664195	1.4045123443260406
9	0.2551031150520222	1.2140695462513689
>10	1.422408478182392	13.551005784837116
>50	0.089816019321068	3.2767918732533254
>100	0.06355243378420482	6.820835240048149
>500	0.0067622555069982715	2.3668295834382613
>1k	0.0026427205429645387	2.086640692749552
>5k	7.772707479307467E-5	0.3540482770119887
>10k+	2.3318122437922405E-4	2.0591437926658487
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24356	0.9954156767751736	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15454	0.6315960695058109	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10289	0.4205054975504911	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8614	0.35204921332490335	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2867	0.11717263694015531	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2843	0.11619177077811704	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2457	0.10041617333866816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	8.173884683652271E-5	0.0
13	0.0	0.0	0.0	8.173884683652271E-5	0.0
14	0.0	0.0	0.0	8.173884683652271E-5	8.173884683652271E-5
15	0.0	0.0	0.0	3.6782481076435224E-4	8.173884683652271E-5
16	0.0	0.0	0.0	8.173884683652272E-4	1.2260827025478406E-4
17	0.0	0.0	0.0	0.0012260827025478407	1.2260827025478406E-4
18	0.0	0.0	0.0	0.0013078215493843633	1.2260827025478406E-4
19	0.0	0.0	0.0	0.0020026017474948066	1.2260827025478406E-4
20	0.0	0.0	0.0	0.0025747736753504655	1.2260827025478406E-4
21	0.0	0.0	0.0	0.0037191175310617836	1.2260827025478406E-4
22	0.0	0.0	0.0	0.005885196972229636	1.2260827025478406E-4
23	0.0	0.0	0.0	0.008541709494416624	2.452165405095681E-4
24	0.0	0.0	0.0	0.011729524521041009	2.860859639278295E-4
25	0.0	0.0	0.0	0.013159954340680157	2.860859639278295E-4
26	0.0	0.0	0.0	0.01687907187174194	3.2695538734609083E-4
27	0.0	0.0	0.0	0.034902487599195196	3.2695538734609083E-4
28	0.0	0.0	0.0	0.06653542132492948	3.2695538734609083E-4
29	0.0	0.0	0.0	0.10765006128370042	3.2695538734609083E-4
30	0.0	0.0	0.0	0.18325849460748392	3.2695538734609083E-4
31	0.0	0.0	0.0	0.4187072429200876	3.2695538734609083E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	665	0.0	17.526316	5
ACGTTAT	75	2.0684679E-4	17.266666	13
CAAGACG	715	0.0	16.81818	4
CTTATAC	1195	0.0	16.25523	37
CCGATAA	485	0.0	16.020618	9
TTAGTAC	130	2.59226E-7	15.653846	3
AACGTTA	95	7.064883E-5	15.578948	12
ACGAACG	500	0.0	15.54	15
CGAACGA	505	0.0	15.386139	16
GGGTAAG	1350	0.0	15.074074	1
ATAACGA	520	0.0	14.942307	12
AACCGAG	100	1.0941652E-4	14.8	7
GACGGAC	775	0.0	14.799999	7
ACGGACC	795	0.0	14.660378	8
CGATAAC	530	0.0	14.6603775	10
TCGTTAC	505	0.0	14.653466	23
TCTTATA	1925	0.0	14.607793	37
ATACCGT	545	0.0	14.59633	6
CCCGTAA	140	6.0023194E-7	14.535715	1
GCACCCG	1360	0.0	14.419118	9
>>END_MODULE