FastQCFastQC Report
Thu 9 Feb 2017
SRR2727221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462727
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT413120.7562523259902975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT259650.4753120556820796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT246660.45153272349139906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127430.23327177067424382No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT80020.1464836152346621No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG67170.12296056530007814No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC60840.11137294614942317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18300.017.9945355
CTTATAC23900.017.72594337
CAAGACG19450.017.5012864
ACGCGTA1251.6609374E-716.2799996
TCTATAC4000.015.7249993
ACGGACC21800.014.8509178
TATACAG9050.014.7182325
GACGGAC21800.014.6811927
TCTTATA38350.014.66492937
TCGTTTA14300.014.36013930
CGCATCG15150.014.28712913
GCGCAAG23750.014.2547371
CGGACCA22450.014.0913139
AGACGGA23550.013.9044596
TAAGTCG800.006302613813.8755
CGCGAAC800.006302613813.87514
CGCAAGA24150.013.8654242
ACGGATG16550.013.74924528
GTCGGGT8650.013.68786230
CGCTTCG11100.013.66666732
Produced by FastQC (version 0.11.5)