##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5462727
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.975220434775526	31.0	31.0	33.0	28.0	34.0
2	31.217554162966593	31.0	31.0	34.0	28.0	34.0
3	31.36394844552913	31.0	31.0	34.0	28.0	34.0
4	35.20577799329895	35.0	35.0	37.0	33.0	37.0
5	35.00947457194914	35.0	35.0	37.0	32.0	37.0
6	35.01871501175145	36.0	35.0	37.0	32.0	37.0
7	34.930598581990274	36.0	35.0	37.0	32.0	37.0
8	34.935253216937255	36.0	35.0	37.0	32.0	37.0
9	36.468698692063505	38.0	35.0	39.0	32.0	39.0
10	36.19380265570657	38.0	35.0	39.0	32.0	39.0
11	36.34443493149118	38.0	35.0	39.0	32.0	39.0
12	36.18538360053505	38.0	35.0	39.0	31.0	39.0
13	36.26062697257249	38.0	35.0	39.0	32.0	39.0
14	37.21389609987832	39.0	36.0	40.0	31.0	41.0
15	37.21557401642074	39.0	36.0	40.0	31.0	41.0
16	37.157380004528875	39.0	36.0	40.0	31.0	41.0
17	37.1373339725745	39.0	36.0	40.0	31.0	41.0
18	37.07314972906389	39.0	36.0	40.0	31.0	41.0
19	37.081289070458766	39.0	36.0	40.0	31.0	41.0
20	36.99777528695833	39.0	36.0	40.0	31.0	41.0
21	36.886766444671316	39.0	36.0	40.0	31.0	41.0
22	36.81499771085028	39.0	36.0	40.0	30.0	41.0
23	36.69251309831152	39.0	35.0	40.0	30.0	41.0
24	36.54260134178406	38.0	35.0	40.0	30.0	41.0
25	36.412362909587095	38.0	35.0	40.0	30.0	41.0
26	36.15491218946142	38.0	35.0	40.0	29.0	41.0
27	35.972232732845704	38.0	34.0	40.0	28.0	41.0
28	35.74598657410484	38.0	34.0	40.0	27.0	41.0
29	35.691443486009824	38.0	34.0	40.0	27.0	41.0
30	35.59069252408184	38.0	34.0	40.0	27.0	41.0
31	35.72046488869021	38.0	34.0	40.0	27.0	41.0
32	35.68889787097177	38.0	34.0	40.0	27.0	41.0
33	35.67173830945607	38.0	34.0	40.0	27.0	41.0
34	35.72258672271194	38.0	34.0	40.0	27.0	41.0
35	35.49476223139102	38.0	34.0	40.0	27.0	41.0
36	35.44334688517292	38.0	34.0	40.0	26.0	41.0
37	35.34818543925039	38.0	34.0	40.0	26.0	41.0
38	35.24594126706314	38.0	33.0	40.0	26.0	41.0
39	35.17348441538448	38.0	33.0	40.0	25.0	41.0
40	34.95271336092761	38.0	33.0	40.0	25.0	41.0
41	34.87349614945063	38.0	33.0	40.0	25.0	41.0
42	34.63711713947997	38.0	33.0	40.0	24.0	41.0
43	34.279831666491845	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	6.0
13	8.0
14	22.0
15	55.0
16	147.0
17	268.0
18	589.0
19	1224.0
20	2573.0
21	5127.0
22	9634.0
23	16503.0
24	26486.0
25	40461.0
26	58844.0
27	82100.0
28	111111.0
29	145144.0
30	182931.0
31	223306.0
32	269641.0
33	323608.0
34	389530.0
35	467157.0
36	572469.0
37	723813.0
38	964568.0
39	845399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70440880534576	19.953605589296338	12.993309019469578	25.34867658588833
2	18.823528981038226	23.37848843627002	32.741376239376415	25.056606343315345
3	20.619573337638876	24.530019530538503	30.298988032900056	24.551419098922572
4	15.256519317183523	16.324227807832976	34.22470132591287	34.19455154907064
5	14.195346024064538	38.260945494805064	33.23675153453578	14.306956946594623
6	34.26081881814705	35.70449337849026	15.45451566589361	14.580172137469068
7	28.211916868626236	32.0474554192439	21.13449930776332	18.606128404366537
8	28.503236570306367	33.00362621086501	19.95928041068133	18.533856808147288
9	27.961986751305712	13.026241289378	19.00393338345482	40.00783857586147
10	16.99202614371906	26.968801479554077	32.312927224809144	23.726245151917713
11	37.02141805731826	21.764221422743624	20.613184587111892	20.601175932826223
12	21.722355885622694	25.842495881635674	29.07793122372764	23.357217009013997
13	30.705030655934294	19.031849843493916	24.99753328328507	25.26558621728671
14	22.985571125923006	20.583053116145106	25.191648786402837	31.239726971529052
15	25.87789944472788	27.503918830283848	22.265710880298432	24.35247084468984
16	24.600259174584416	25.80665297753302	24.930680226194717	24.662407621687848
17	23.9633062388071	25.992439307327643	25.602304490046823	24.441949963818438
18	24.068693163689126	24.18542973134114	27.343394608590177	24.402482496379555
19	24.428568368875105	25.297676416925103	27.329024496373332	22.944730717826463
20	24.20221621911547	23.978811315300945	26.974915641949526	24.844056823634055
21	24.97913587847242	25.085200120745554	26.828962897102492	23.106701103679537
22	25.160711856916883	24.508565044528126	26.071648830336937	24.259074268218054
23	23.540916469008977	24.572306102794446	26.90136263444979	24.985414793746784
24	23.614982041020905	25.466145388557766	26.76309835728566	24.155774213135675
25	24.39966339156249	24.26301003143668	26.18168544757957	25.15564112942126
26	23.82566436140777	25.425854156724288	26.89982494091321	23.848656540954728
27	25.10608712461743	24.837759602484255	25.992951871839832	24.063201401058482
28	23.628400247715106	25.556905918966844	26.927192224689243	23.887501608628803
29	23.805271616172654	25.429680084690304	27.09655818421825	23.668490114918793
30	23.98988636993941	25.88857177010676	26.847964395804514	23.273577464149316
31	24.296564701109904	25.53508531544776	26.134950547592805	24.03339943584953
32	23.509704951391495	25.6138005798203	26.139435487074497	24.737058981713712
33	23.223877012342005	25.125070317444013	26.896841083217225	24.754211586996753
34	24.196248503723506	25.351568914207135	27.067634901030203	23.38454768103916
35	24.182830297029305	25.23344110002202	27.333179930097184	23.25054867285149
36	23.28293176649684	25.77844362348695	26.86969346994642	24.068931140069786
37	23.612419218459937	25.54070521920645	27.18997306656547	23.65690249576814
38	23.501668672075322	24.819600174052265	27.371860244892343	24.306870908980073
39	23.594955413294496	24.63211139784214	27.343779031974325	24.42915415688904
40	23.80318474637301	24.678901215455213	27.848380488353165	23.669533549818617
41	22.561753497840915	25.079488687609686	28.232730648996373	24.12602716555303
42	23.491417381831457	24.969873105502067	28.058239044345434	23.48047046832104
43	22.751585426106775	23.893597465148815	28.204210095067907	25.150607013676503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	906.0
1	917.0
2	928.0
3	2030.0
4	3132.0
5	3132.0
6	5336.0
7	7540.0
8	7199.5
9	6859.0
10	9286.5
11	11714.0
12	11714.0
13	21767.5
14	31821.0
15	54030.0
16	76239.0
17	65413.5
18	54588.0
19	54588.0
20	57848.0
21	61108.0
22	49246.0
23	37384.0
24	43761.5
25	50139.0
26	50139.0
27	58718.0
28	67297.0
29	76240.5
30	85184.0
31	96093.0
32	107002.0
33	107002.0
34	123735.5
35	140469.0
36	166969.5
37	193470.0
38	219959.0
39	246448.0
40	246448.0
41	273830.5
42	301213.0
43	320058.5
44	338904.0
45	372061.5
46	405219.0
47	405219.0
48	455475.0
49	505731.0
50	506859.5
51	507988.0
52	478958.5
53	449929.0
54	449929.0
55	431668.5
56	413408.0
57	394543.5
58	375679.0
59	347595.0
60	319511.0
61	319511.0
62	269255.5
63	219000.0
64	191063.0
65	163126.0
66	136707.5
67	110289.0
68	110289.0
69	90161.0
70	70033.0
71	58312.5
72	46592.0
73	34010.5
74	21429.0
75	21429.0
76	16601.0
77	11773.0
78	10244.0
79	8715.0
80	6820.5
81	4926.0
82	4926.0
83	4077.5
84	3229.0
85	2841.5
86	2454.0
87	1587.5
88	721.0
89	721.0
90	508.5
91	296.0
92	233.0
93	170.0
94	155.5
95	141.0
96	141.0
97	73.5
98	6.0
99	11.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5462727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.18576388592844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37167450025314	34.27726394198699
2	10.073054540345979	8.700251099789218
3	3.489076090207245	4.520352486351853
4	1.7692270673887474	3.056216895713762
5	1.099914253920947	2.375031863229858
6	0.7263349685006084	1.882039827105633
7	0.5217732736601435	1.5773224178792418
8	0.3870407858047349	1.3371721591989987
9	0.3024757358154077	1.1756381137331997
>10	1.9991782580519042	16.25034121337199
>50	0.1520999582568712	4.522928317106833
>100	0.08705894778858182	7.23955868871171
>500	0.011608898266743432	3.522375329607504
>1k	0.009185057827833494	7.25901987320039
>5k	1.275702476086998E-4	0.38202758123810004
>10k+	1.7009366347826637E-4	1.9224601917748279
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41312	0.7562523259902975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25965	0.4753120556820796	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24666	0.45153272349139906	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12743	0.23327177067424382	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	8002	0.1464836152346621	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6717	0.12296056530007814	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	6084	0.11137294614942317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8305875435473894E-5	3.661175087094779E-5
2	0.0	0.0	0.0	1.8305875435473894E-5	3.661175087094779E-5
3	0.0	0.0	0.0	1.8305875435473894E-5	5.4917626306421686E-5
4	0.0	0.0	0.0	1.8305875435473894E-5	5.4917626306421686E-5
5	0.0	0.0	0.0	1.8305875435473894E-5	5.4917626306421686E-5
6	0.0	0.0	0.0	1.8305875435473894E-5	5.4917626306421686E-5
7	0.0	0.0	0.0	1.8305875435473894E-5	5.4917626306421686E-5
8	0.0	0.0	0.0	1.8305875435473894E-5	5.4917626306421686E-5
9	0.0	0.0	0.0	1.8305875435473894E-5	9.152937717736947E-5
10	0.0	0.0	0.0	1.8305875435473894E-5	9.152937717736947E-5
11	0.0	0.0	0.0	1.8305875435473894E-5	9.152937717736947E-5
12	0.0	0.0	0.0	3.661175087094779E-5	9.152937717736947E-5
13	0.0	0.0	0.0	5.4917626306421686E-5	1.0983525261284337E-4
14	0.0	0.0	0.0	1.0983525261284337E-4	1.4644700348379115E-4
15	0.0	1.8305875435473894E-5	0.0	1.4644700348379115E-4	2.0136462979021284E-4
16	0.0	1.8305875435473894E-5	0.0	3.111998824030562E-4	2.562822560966345E-4
17	0.0	1.8305875435473894E-5	0.0	6.407056402415863E-4	2.562822560966345E-4
18	0.0	1.8305875435473894E-5	0.0	7.871526437253774E-4	3.111998824030562E-4
19	0.0	1.8305875435473894E-5	0.0	0.0011349642769993814	3.111998824030562E-4
20	0.0	1.8305875435473894E-5	0.0	0.0014278582839669638	3.111998824030562E-4
21	0.0	1.8305875435473894E-5	0.0	0.0021967050522568674	3.111998824030562E-4
22	0.0	1.8305875435473894E-5	0.0	0.0031852223257724578	3.111998824030562E-4
23	0.0	1.8305875435473894E-5	0.0	0.005436845004335746	3.47811633274004E-4
24	0.0	1.8305875435473894E-5	0.0	0.007908138188124723	3.6611750870947787E-4
25	0.0	3.661175087094779E-5	0.0	0.008988184838817682	3.6611750870947787E-4
26	0.0	3.661175087094779E-5	0.0	0.011715760278703292	3.6611750870947787E-4
27	0.0	3.661175087094779E-5	0.0	0.02317523830130995	3.844233841449518E-4
28	0.0	3.661175087094779E-5	0.0	0.0450873711975722	3.844233841449518E-4
29	0.0	3.661175087094779E-5	0.0	0.07675653570094204	3.844233841449518E-4
30	0.0	3.661175087094779E-5	0.0	0.1300815508444775	3.844233841449518E-4
31	0.0	3.661175087094779E-5	0.0	0.3130121640711681	4.027292595804257E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1830	0.0	17.994535	5
CTTATAC	2390	0.0	17.725943	37
CAAGACG	1945	0.0	17.501286	4
ACGCGTA	125	1.6609374E-7	16.279999	6
TCTATAC	400	0.0	15.724999	3
ACGGACC	2180	0.0	14.850917	8
TATACAG	905	0.0	14.718232	5
GACGGAC	2180	0.0	14.681192	7
TCTTATA	3835	0.0	14.664929	37
TCGTTTA	1430	0.0	14.360139	30
CGCATCG	1515	0.0	14.287129	13
GCGCAAG	2375	0.0	14.254737	1
CGGACCA	2245	0.0	14.091313	9
AGACGGA	2355	0.0	13.904459	6
TAAGTCG	80	0.0063026138	13.875	5
CGCGAAC	80	0.0063026138	13.875	14
CGCAAGA	2415	0.0	13.865424	2
ACGGATG	1655	0.0	13.749245	28
GTCGGGT	865	0.0	13.687862	30
CGCTTCG	1110	0.0	13.666667	32
>>END_MODULE