FastQCFastQC Report
Thu 9 Feb 2017
SRR2727220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4463056
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT334980.7505619467916155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT215150.48206878874027126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT201100.4505881172004116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102840.2304250719686242No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG61540.13788758196177686No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC58240.13049354522999487No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT54990.12321153935778535No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA54410.12191198138674487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA752.06895E-417.26666614
AAGACGG13250.017.1735865
CAAGACG13350.016.6292134
TACCGTC9250.016.07
ATACCGT10150.015.3103456
TACGGAT16900.015.21597627
TCTTATA32450.015.10785837
GACGGAC14600.015.0787677
TCTATAC3350.014.9104483
ACGGATG18250.014.59726128
ACCGTCG10150.014.3990148
GCGCAAG16100.014.3633551
ACGGACC15200.014.3618428
CTTATAC19250.014.3194837
TATGCCG1702.4421752E-814.1470595
TCGTTAC9300.014.12365523
CGGACCA15950.013.8025089
CGTCGTA10700.013.48598110
CGCAAGA16350.013.4648322
CGTTACG7900.013.34810124
Produced by FastQC (version 0.11.5)