##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727220.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4463056
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199168686209628	31.0	31.0	34.0	30.0	34.0
2	31.42919896142912	31.0	31.0	34.0	30.0	34.0
3	31.580911375523858	31.0	31.0	34.0	30.0	34.0
4	35.398141766538444	37.0	35.0	37.0	33.0	37.0
5	35.213113391362334	37.0	35.0	37.0	33.0	37.0
6	35.212460027389305	37.0	35.0	37.0	32.0	37.0
7	35.11129660931881	37.0	35.0	37.0	32.0	37.0
8	35.11836329187893	37.0	35.0	37.0	32.0	37.0
9	36.67918798240488	39.0	35.0	39.0	32.0	39.0
10	36.412005809472255	38.0	35.0	39.0	32.0	39.0
11	36.56667202921048	38.0	35.0	39.0	32.0	39.0
12	36.42176257703242	38.0	35.0	39.0	32.0	39.0
13	36.48352406960612	38.0	35.0	39.0	32.0	39.0
14	37.487874900068476	39.0	36.0	41.0	32.0	41.0
15	37.481246930354445	39.0	36.0	41.0	32.0	41.0
16	37.42752835725118	39.0	36.0	41.0	32.0	41.0
17	37.420049625189556	39.0	36.0	41.0	32.0	41.0
18	37.35208901703228	39.0	36.0	41.0	32.0	41.0
19	37.370845447603614	39.0	36.0	41.0	31.0	41.0
20	37.29489726322054	39.0	36.0	41.0	31.0	41.0
21	37.191108513986826	39.0	36.0	40.0	31.0	41.0
22	37.12837212887313	39.0	36.0	40.0	31.0	41.0
23	37.015199898903354	39.0	36.0	40.0	31.0	41.0
24	36.886029886248345	39.0	36.0	40.0	30.0	41.0
25	36.75832590942171	39.0	35.0	40.0	30.0	41.0
26	36.525132106789606	38.0	35.0	40.0	30.0	41.0
27	36.35566459394639	38.0	35.0	40.0	30.0	41.0
28	36.14037287455053	38.0	35.0	40.0	29.0	41.0
29	36.07391146335605	38.0	35.0	40.0	29.0	41.0
30	35.97486699696351	38.0	34.0	40.0	29.0	41.0
31	36.089377323520026	38.0	35.0	40.0	30.0	41.0
32	36.05495337723748	38.0	35.0	40.0	29.0	41.0
33	36.03982472996081	38.0	35.0	40.0	29.0	41.0
34	36.077900882265425	38.0	35.0	40.0	29.0	41.0
35	35.86092377061816	38.0	34.0	40.0	28.0	41.0
36	35.8082867434332	38.0	34.0	40.0	27.0	41.0
37	35.72186098493947	38.0	34.0	40.0	27.0	41.0
38	35.60852093274205	38.0	34.0	40.0	27.0	41.0
39	35.52343932050147	38.0	34.0	40.0	27.0	41.0
40	35.31937757446915	38.0	34.0	40.0	26.0	41.0
41	35.23070066788317	38.0	34.0	40.0	26.0	41.0
42	35.010247238663375	38.0	33.0	40.0	25.0	41.0
43	34.64173180887715	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	5.0
14	13.0
15	29.0
16	70.0
17	184.0
18	338.0
19	722.0
20	1619.0
21	3252.0
22	5923.0
23	10528.0
24	17263.0
25	27041.0
26	39819.0
27	57170.0
28	79344.0
29	104876.0
30	134965.0
31	167886.0
32	205804.0
33	249399.0
34	306434.0
35	370722.0
36	455624.0
37	589576.0
38	785676.0
39	848771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96468608056901	18.874040567718623	12.481358064967143	25.679915286745224
2	18.04252512179995	22.875334748208402	33.739796229310144	25.342343900681506
3	20.15058740020291	24.49953574411793	30.22619478671117	25.12368206896799
4	14.653255527154489	15.643361857883924	34.05332579290961	35.65005682205197
5	14.20204003714047	38.24675289756615	33.08040499603859	14.470802069254788
6	34.51619249231916	34.85967910776831	15.814746666857864	14.80938173305466
7	28.355682742945643	32.05507616305957	21.03453328840149	18.5547078055933
8	28.785209058546428	32.90908740558039	19.94857335422186	18.357130181651318
9	28.661078866140148	12.939900373197199	18.819347102075348	39.579673658587296
10	16.958850617155598	27.113215697943293	32.08637758522411	23.841556099676993
11	36.80390745713251	22.09436762612882	20.33319770130601	20.768527215432655
12	21.98509272570185	25.745744619830003	28.857894680237038	23.41126797423111
13	30.88753535693928	19.059294797107633	24.57820381370971	25.47496603224338
14	23.106544036194034	20.671284429323762	25.152003470267907	31.0701680642143
15	26.23953183648155	27.295086595373213	22.205076521558322	24.260305046586915
16	24.468592820704018	25.82871467442936	25.02957614692713	24.67311635793949
17	24.13017000010755	25.914351063486546	25.38375050637949	24.571728430026422
18	24.013030533338593	24.53534080683729	27.181957833376952	24.26967082644717
19	24.520754388920952	25.548749556357798	27.165556515535545	22.764939539185704
20	24.074423444384298	24.40312198636988	26.809231163579394	24.713223405666433
21	24.898970570837562	25.441222337340154	26.83264561323004	22.82716147859225
22	25.100670930411805	24.752434206516792	25.605325140441888	24.541569722629518
23	23.464684288075254	25.085389921166122	26.62045916519981	24.829466625558812
24	23.46750746573648	25.82703421153577	26.479546750029577	24.22591157269817
25	24.35044507619891	24.568882846193283	25.772363152064415	25.308308925543393
26	23.739047863168196	25.74505002850065	26.67479413209245	23.841107976238703
27	24.943334791228253	25.4431492681248	25.597707041990958	24.015808898655987
28	23.31946988789744	26.449096762397783	26.247261965792053	23.984171383912727
29	23.65699646161733	26.10406412108654	26.76392588396829	23.47501353332784
30	24.09449937441968	26.395501199178323	26.34840342581406	23.161596000587938
31	24.229339717001086	26.11372118118168	25.76575333134964	23.89118577046759
32	23.202554482847624	26.325750786008513	25.792528706787454	24.679166024356405
33	22.939169931992787	25.72434672565166	26.580217680441383	24.756265661914167
34	23.869899907148824	25.985557877830797	26.886218770277587	23.258323444742796
35	23.91179944862892	25.9639135157614	26.886868549263106	23.237418486346577
36	23.125118752711145	26.33538543993174	26.640893593985826	23.898602213371287
37	23.272013615782548	26.138367970287625	27.018796089495627	23.5708223244342
38	23.348530692870536	25.46687740418225	27.036698620855304	24.14789328209191
39	23.115461692616	25.258208725142595	27.2890369289563	24.337292653285104
40	23.690157596050778	25.181041869069087	27.57915204290513	23.549648491975006
41	22.333351855768782	25.684194865580896	27.997968208330793	23.98448507031953
42	23.100942493215413	25.670840787119857	27.803975571895133	23.4242411477696
43	22.335995784054692	24.55965150336451	27.891449267049307	25.21290344553149
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	436.0
1	558.0
2	680.0
3	1845.5
4	3011.0
5	3011.0
6	4803.5
7	6596.0
8	6306.5
9	6017.0
10	8036.0
11	10055.0
12	10055.0
13	18903.0
14	27751.0
15	46573.0
16	65395.0
17	57119.0
18	48843.0
19	48843.0
20	50982.5
21	53122.0
22	42997.5
23	32873.0
24	38713.5
25	44554.0
26	44554.0
27	51917.5
28	59281.0
29	65532.0
30	71783.0
31	79011.5
32	86240.0
33	86240.0
34	98925.5
35	111611.0
36	131850.5
37	152090.0
38	173910.0
39	195730.0
40	195730.0
41	216218.5
42	236707.0
43	252430.5
44	268154.0
45	296713.5
46	325273.0
47	325273.0
48	369294.5
49	413316.0
50	419919.5
51	426523.0
52	398901.0
53	371279.0
54	371279.0
55	353322.5
56	335366.0
57	320589.5
58	305813.0
59	281670.0
60	257527.0
61	257527.0
62	216702.5
63	175878.0
64	153279.5
65	130681.0
66	110422.5
67	90164.0
68	90164.0
69	74324.0
70	58484.0
71	49228.0
72	39972.0
73	30144.5
74	20317.0
75	20317.0
76	15969.0
77	11621.0
78	10044.5
79	8468.0
80	6697.0
81	4926.0
82	4926.0
83	4021.5
84	3117.0
85	2655.5
86	2194.0
87	1445.5
88	697.0
89	697.0
90	479.0
91	261.0
92	185.0
93	109.0
94	117.0
95	125.0
96	125.0
97	65.5
98	6.0
99	8.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4463056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.202867966452025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.71713589451613	38.100803082590105
2	9.769847693018562	9.223296614118023
3	3.33607357528807	4.724167215020769
4	1.6700743102959894	3.153291886522601
5	0.9972238620436922	2.3535913146521885
6	0.6388816259231602	1.8094227020785878
7	0.4618920432197986	1.5261840391601234
8	0.34590766367344455	1.3062267021569196
9	0.25208332206975465	1.070915019138291
>10	1.5986356231329977	13.836943323761192
>50	0.11784174752173858	3.834956948451536
>100	0.07514937909004303	7.171741029370164
>500	0.012009459477810283	3.9891545708830285
>1k	0.006862548059006776	5.462504963377963
>5k	1.906263349724095E-4	0.5155466964435705
>10k+	1.906263349724095E-4	1.9212538922748945
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33498	0.7505619467916155	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21515	0.48206878874027126	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20110	0.4505881172004116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10284	0.2304250719686242	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6154	0.13788758196177686	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	5824	0.13049354522999487	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5499	0.12321153935778535	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	5441	0.12191198138674487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2406171914490878E-5
2	0.0	0.0	0.0	0.0	2.2406171914490878E-5
3	0.0	0.0	0.0	0.0	2.2406171914490878E-5
4	0.0	0.0	0.0	0.0	2.2406171914490878E-5
5	0.0	0.0	0.0	0.0	2.2406171914490878E-5
6	0.0	0.0	0.0	0.0	4.4812343828981756E-5
7	0.0	0.0	0.0	0.0	1.1203085957245439E-4
8	0.0	0.0	0.0	0.0	1.3443703148694527E-4
9	0.0	0.0	0.0	0.0	1.7924937531592702E-4
10	0.0	0.0	0.0	0.0	1.7924937531592702E-4
11	0.0	0.0	0.0	0.0	1.7924937531592702E-4
12	0.0	0.0	0.0	0.0	2.2406171914490878E-4
13	0.0	0.0	0.0	0.0	2.2406171914490878E-4
14	0.0	0.0	0.0	6.721851574347263E-5	3.136864068028723E-4
15	0.0	0.0	0.0	1.5684320340143615E-4	3.5849875063185405E-4
16	0.0	0.0	0.0	5.825604697767628E-4	3.809049225463449E-4
17	0.0	0.0	0.0	8.962468765796351E-4	4.033110944608358E-4
18	0.0	0.0	0.0	0.0011427147676390348	4.257172663753267E-4
19	0.0	0.0	0.0	0.001658056721672325	4.257172663753267E-4
20	0.0	0.0	0.0	0.002218211019534597	4.7052961020430844E-4
21	0.0	0.0	0.0	0.003450550474831595	4.929357821187993E-4
22	0.0	0.0	0.0	0.0053102627437343385	4.929357821187993E-4
23	0.0	0.0	0.0	0.00864878235899348	6.049666416912537E-4
24	0.0	0.0	0.0	0.013331672289122073	6.049666416912537E-4
25	0.0	0.0	0.0	0.015258603073768287	6.049666416912537E-4
26	0.0	0.0	0.0	0.01893321526774479	6.049666416912537E-4
27	0.0	0.0	0.0	0.033093915917703026	6.049666416912537E-4
28	0.0	0.0	0.0	0.05989169752743412	6.049666416912537E-4
29	0.0	0.0	0.0	0.09567435407487605	6.049666416912537E-4
30	0.0	0.0	0.0	0.15529717753933628	6.049666416912537E-4
31	0.0	0.0	0.0	0.36739400088190693	6.049666416912537E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	75	2.06895E-4	17.266666	14
AAGACGG	1325	0.0	17.173586	5
CAAGACG	1335	0.0	16.629213	4
TACCGTC	925	0.0	16.0	7
ATACCGT	1015	0.0	15.310345	6
TACGGAT	1690	0.0	15.215976	27
TCTTATA	3245	0.0	15.107858	37
GACGGAC	1460	0.0	15.078767	7
TCTATAC	335	0.0	14.910448	3
ACGGATG	1825	0.0	14.597261	28
ACCGTCG	1015	0.0	14.399014	8
GCGCAAG	1610	0.0	14.363355	1
ACGGACC	1520	0.0	14.361842	8
CTTATAC	1925	0.0	14.31948	37
TATGCCG	170	2.4421752E-8	14.147059	5
TCGTTAC	930	0.0	14.123655	23
CGGACCA	1595	0.0	13.802508	9
CGTCGTA	1070	0.0	13.485981	10
CGCAAGA	1635	0.0	13.464832	2
CGTTACG	790	0.0	13.348101	24
>>END_MODULE