FastQCFastQC Report
Thu 9 Feb 2017
SRR2727219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6003751
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277250.46179463472086035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT196190.3267790419689291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178570.2974307228930714No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT117610.19589420014254422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87680.14604203272254296No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT80610.13426606133398938No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG75590.12590462196050436No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC75080.12505515302017023No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC74340.12382259024399914No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG73280.1220570273484027No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG71910.11977512058711295No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA70550.11750987007955527No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC70060.11669371364668522No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG69470.11571099467649473No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG68590.11424524434807505No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA67560.11252965021367475No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG67140.11183008755692898No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA66770.11121380616884345No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG64820.10796583669109529No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG64490.1074161803179379No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC63830.10631686757162315No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC62780.10456796092975876No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG62250.10368517948196052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG25150.020.228635
CAAGACG25400.020.1751984
CTTATAC23600.017.63771237
ACGGACC29400.017.0527218
GTATACG1201.04333594E-716.9583321
GACGGAC29950.016.8013347
AGACGGA31850.016.205656
CGGACCA31450.016.0588239
CGCAAGA31850.015.9733112
GCGCAAG31450.015.9411761
TCTATAC4400.015.5568183
ATACCGT19100.015.3036646
AGAGCGA32450.015.27889115
GCAAGAC36600.015.1133873
TACCGTC19050.015.0524937
ACCGTCG19300.014.9533688
TCGTTTA19100.014.9162330
GAGCGAA33500.014.85522416
CAGAGCG34000.014.814
TCTTATA37950.014.67325437
Produced by FastQC (version 0.11.5)