##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727219.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6003751
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232862422175735	31.0	31.0	34.0	30.0	34.0
2	31.467978768606493	31.0	31.0	34.0	30.0	34.0
3	31.627557671862142	31.0	31.0	34.0	30.0	34.0
4	35.430894452484786	37.0	35.0	37.0	33.0	37.0
5	35.25279096351598	37.0	35.0	37.0	33.0	37.0
6	35.2524884859482	37.0	35.0	37.0	32.0	37.0
7	35.15656828539358	37.0	35.0	37.0	32.0	37.0
8	35.15526243510099	37.0	35.0	37.0	32.0	37.0
9	36.7357085595322	39.0	35.0	39.0	32.0	39.0
10	36.467480746619906	38.0	35.0	39.0	32.0	39.0
11	36.61697761949155	39.0	35.0	39.0	32.0	39.0
12	36.463501900728396	38.0	35.0	39.0	32.0	39.0
13	36.52749206287869	38.0	35.0	39.0	32.0	39.0
14	37.554597950514605	39.0	36.0	41.0	32.0	41.0
15	37.550890268433854	39.0	36.0	41.0	32.0	41.0
16	37.49723364609891	39.0	36.0	41.0	32.0	41.0
17	37.46226584013894	39.0	36.0	41.0	32.0	41.0
18	37.383043367388154	39.0	36.0	41.0	32.0	41.0
19	37.38313780834682	39.0	36.0	41.0	31.0	41.0
20	37.30161294164265	39.0	36.0	41.0	31.0	41.0
21	37.19549311755268	39.0	36.0	40.0	31.0	41.0
22	37.12002962814414	39.0	36.0	40.0	31.0	41.0
23	36.99557676525892	39.0	36.0	40.0	31.0	41.0
24	36.84412994476286	39.0	36.0	40.0	30.0	41.0
25	36.718537794122376	39.0	35.0	40.0	30.0	41.0
26	36.49672096660904	38.0	35.0	40.0	30.0	41.0
27	36.3203887036621	38.0	35.0	40.0	30.0	41.0
28	36.06875718196841	38.0	35.0	40.0	29.0	41.0
29	36.01015248633729	38.0	35.0	40.0	29.0	41.0
30	35.919386563500055	38.0	34.0	40.0	28.0	41.0
31	36.085589991990005	38.0	35.0	40.0	29.0	41.0
32	36.064546647587484	38.0	35.0	40.0	29.0	41.0
33	36.05018978968315	38.0	35.0	40.0	29.0	41.0
34	36.09644970286076	38.0	35.0	40.0	29.0	41.0
35	35.867183532428314	38.0	34.0	40.0	28.0	41.0
36	35.83334035672032	38.0	34.0	40.0	27.0	41.0
37	35.758059752977765	38.0	34.0	40.0	27.0	41.0
38	35.64211090699798	38.0	34.0	40.0	27.0	41.0
39	35.572642169870136	38.0	34.0	40.0	27.0	41.0
40	35.37045856831837	38.0	34.0	40.0	26.0	41.0
41	35.28319329032799	38.0	34.0	40.0	26.0	41.0
42	35.07062234926132	38.0	33.0	40.0	25.0	41.0
43	34.70649249111097	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	8.0
14	18.0
15	57.0
16	166.0
17	331.0
18	570.0
19	1144.0
20	2398.0
21	4753.0
22	8661.0
23	14807.0
24	23597.0
25	36294.0
26	53537.0
27	76160.0
28	104300.0
29	138944.0
30	178728.0
31	222935.0
32	272571.0
33	332181.0
34	406511.0
35	495347.0
36	613938.0
37	789547.0
38	1079726.0
39	1146517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98134616175787	18.89340513955359	12.039173510027315	26.086075188661223
2	18.537477653553587	22.3788594830132	33.306078150143136	25.777584713290075
3	20.07246802873737	24.100749681324228	29.82745287071366	25.999329419224747
4	14.36023912384108	15.461234151782776	34.71398130935144	35.46454541502471
5	14.406859978037065	37.706060761014236	33.313590120576286	14.573489140372411
6	34.724191592889184	35.39968596299213	15.591652618504664	14.284469825614021
7	28.934044733034398	31.64216837107335	21.039530120419716	18.384256775472537
8	28.524767266330663	32.653735972727716	19.97263044386751	18.84886631707411
9	28.35327447790556	12.975837938648688	18.621575078646664	40.04931250479908
10	17.057053165595974	26.03752220903232	31.964117099459987	24.941307525911718
11	37.798786125540516	21.39850570085268	20.509111720322846	20.293596453283953
12	22.188911565452997	25.228044933908816	28.5956895947217	23.987353905916482
13	30.39683024828978	18.45071522786338	24.974520095853407	26.17793442799343
14	23.279746278618152	20.460642021962602	24.274824189077794	31.98478751034145
15	26.121936102946307	27.32360152844447	21.61478715556325	24.939675213045977
16	25.460832736067836	25.443943294783544	24.140558127743805	24.954665841404815
17	24.582315289225022	25.608257237850136	24.63476583222722	25.174661640697625
18	24.983864254197087	23.865496753612867	26.39716403961457	24.753474952575484
19	25.24078696801383	25.061282521543614	25.913383149967412	23.78454736047514
20	24.86890279093853	23.729431816875817	25.48803239841226	25.913632993773394
21	26.084959219661176	24.8834936692078	25.474191051560936	23.557356059570093
22	25.81604400315736	24.072617268770806	24.929531554523162	25.18180717354867
23	23.930606049451416	24.134178782564433	25.910484961818035	26.02473020616611
24	24.163327226595506	25.180458016996376	25.689806256122218	24.966408500285905
25	25.04779095602066	23.983689530095436	25.002136164541135	25.96638334934277
26	24.461857262234894	25.445825451455267	25.77438671257352	24.31793057373632
27	26.082893844198402	24.49063926868386	24.689531594498174	24.736935292619563
28	24.175036572969134	25.415527725916682	25.792725247932502	24.616710453181685
29	24.241195212792803	25.332129863480347	26.028011488151325	24.398663435575525
30	24.578217850806936	25.72778251463127	25.846508291233267	23.847491343328528
31	24.972987720510062	25.412729475289698	24.760387297874278	24.853895506325962
32	24.26189893618173	25.29038929162785	24.668011714676375	25.77970005751404
33	23.682527806366387	24.650589273272658	25.722735669750463	25.944147250610495
34	25.182440111190484	24.971505313927906	25.907020461041775	23.93903411383983
35	25.34225686574943	24.671825996781013	26.18289799160558	23.803019145863978
36	23.813928992058464	25.656860186240237	25.81346228382889	24.715748537872408
37	24.53089743395421	25.293887104911576	26.02489676870343	24.150318692430783
38	24.12876550010152	24.4419530390251	26.42403057688435	25.005250883989028
39	24.458809167801927	24.23594849286721	26.104013973930627	25.20122836540023
40	24.83267543907134	24.35529055085729	26.718846267941494	24.093187742129878
41	23.102940145252525	25.05470330131946	27.295185959577605	24.547170593850414
42	24.669377527482403	24.80184471341333	26.968040480026573	23.560737279077696
43	23.53162214755409	23.549777464122013	27.042560559223727	25.876039829100172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	568.0
1	636.5
2	705.0
3	1811.0
4	2917.0
5	2917.0
6	4713.0
7	6509.0
8	6120.5
9	5732.0
10	7505.0
11	9278.0
12	9278.0
13	16616.5
14	23955.0
15	39278.5
16	54602.0
17	49458.5
18	44315.0
19	44315.0
20	46637.5
21	48960.0
22	41532.0
23	34104.0
24	40566.0
25	47028.0
26	47028.0
27	55128.5
28	63229.0
29	72080.0
30	80931.0
31	91119.5
32	101308.0
33	101308.0
34	121048.5
35	140789.0
36	172641.0
37	204493.0
38	236128.0
39	267763.0
40	267763.0
41	297031.5
42	326300.0
43	343055.5
44	359811.0
45	399890.0
46	439969.0
47	439969.0
48	498167.5
49	556366.0
50	558853.0
51	561340.0
52	530920.0
53	500500.0
54	500500.0
55	485047.0
56	469594.0
57	452467.5
58	435341.0
59	409467.0
60	383593.0
61	383593.0
62	319221.0
63	254849.0
64	227343.0
65	199837.0
66	170229.0
67	140621.0
68	140621.0
69	115989.5
70	91358.0
71	78570.5
72	65783.0
73	48164.5
74	30546.0
75	30546.0
76	23736.0
77	16926.0
78	15029.0
79	13132.0
80	10641.5
81	8151.0
82	8151.0
83	6958.5
84	5766.0
85	5049.0
86	4332.0
87	2836.0
88	1340.0
89	1340.0
90	940.5
91	541.0
92	399.0
93	257.0
94	275.0
95	293.0
96	293.0
97	152.0
98	11.0
99	9.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6003751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.932961820835814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48894318138444	32.53717876422196
2	10.161246608853666	8.31859838984609
3	3.551615658238727	4.361344444229054
4	1.755928004414384	2.8750133585932156
5	1.0727379825232397	2.19551714411921
6	0.7218522351918446	1.7728529990035662
7	0.5096090350318396	1.4601865022157892
8	0.36956397840792976	1.2101878574822384
9	0.2880803680642396	1.0612784436575262
>10	1.828249983331134	13.922010662832143
>50	0.13466417503768122	3.7550197322191106
>100	0.08788147466732184	7.160660093050944
>500	0.014036149488416126	4.003563142933062
>1k	0.014363514549226955	11.21027974551417
>5k	0.0010639640406821858	2.8671597152621877
>10k+	1.6368677548956708E-4	1.2891490048198477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27725	0.46179463472086035	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19619	0.3267790419689291	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17857	0.2974307228930714	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	11761	0.19589420014254422	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8768	0.14604203272254296	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	8061	0.13426606133398938	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	7559	0.12590462196050436	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	7508	0.12505515302017023	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	7434	0.12382259024399914	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	7328	0.1220570273484027	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	7191	0.11977512058711295	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	7055	0.11750987007955527	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	7006	0.11669371364668522	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	6947	0.11571099467649473	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	6859	0.11424524434807505	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	6756	0.11252965021367475	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	6714	0.11183008755692898	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA	6677	0.11121380616884345	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6482	0.10796583669109529	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	6449	0.1074161803179379	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	6383	0.10631686757162315	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	6278	0.10456796092975876	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	6225	0.10368517948196052	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.665625373204185E-5
2	0.0	0.0	0.0	0.0	1.665625373204185E-5
3	0.0	0.0	0.0	0.0	1.665625373204185E-5
4	0.0	0.0	0.0	0.0	3.33125074640837E-5
5	0.0	0.0	0.0	0.0	3.33125074640837E-5
6	0.0	0.0	1.665625373204185E-5	1.665625373204185E-5	6.66250149281674E-5
7	0.0	0.0	1.665625373204185E-5	1.665625373204185E-5	6.66250149281674E-5
8	0.0	0.0	1.665625373204185E-5	1.665625373204185E-5	6.66250149281674E-5
9	0.0	0.0	1.665625373204185E-5	3.33125074640837E-5	9.993752239225111E-5
10	0.0	0.0	1.665625373204185E-5	3.33125074640837E-5	9.993752239225111E-5
11	0.0	0.0	1.665625373204185E-5	3.33125074640837E-5	9.993752239225111E-5
12	0.0	0.0	1.665625373204185E-5	6.66250149281674E-5	9.993752239225111E-5
13	0.0	0.0	1.665625373204185E-5	9.993752239225111E-5	9.993752239225111E-5
14	0.0	0.0	1.665625373204185E-5	1.6656253732041853E-4	1.332500298563348E-4
15	0.0	0.0	1.665625373204185E-5	2.831563134447115E-4	1.8321879105246037E-4
16	0.0	0.0	1.665625373204185E-5	6.662501492816741E-4	2.831563134447115E-4
17	0.0	0.0	1.665625373204185E-5	0.0010660002388506785	2.831563134447115E-4
18	0.0	0.0	1.665625373204185E-5	0.0011992502687070132	3.3312507464083706E-4
19	0.0	0.0	1.665625373204185E-5	0.001565687850811934	3.3312507464083706E-4
20	0.0	0.0	1.665625373204185E-5	0.0019654379403809385	3.497813283728789E-4
21	0.0	0.0	1.665625373204185E-5	0.0027649381195189475	3.6643758210492075E-4
22	0.0	0.0	1.665625373204185E-5	0.0039641883882259605	3.6643758210492075E-4
23	0.0	0.0	1.665625373204185E-5	0.005679782522626272	4.830313582292137E-4
24	0.0	0.0	1.665625373204185E-5	0.008411408134681135	4.996876119612555E-4
25	0.0	0.0	1.665625373204185E-5	0.009610658403388149	5.163438656932974E-4
26	0.0	0.0	1.665625373204185E-5	0.012092440209462384	5.163438656932974E-4
27	0.0	0.0	1.665625373204185E-5	0.0248344743144744	5.330001194253392E-4
28	0.0	0.0	1.665625373204185E-5	0.04718716682287457	5.330001194253392E-4
29	0.0	0.0	1.665625373204185E-5	0.07861751761523754	5.330001194253392E-4
30	0.0	0.0	1.665625373204185E-5	0.13070162303533242	5.330001194253392E-4
31	0.0	0.0	1.665625373204185E-5	0.31217150744592836	5.330001194253392E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	2515	0.0	20.22863	5
CAAGACG	2540	0.0	20.175198	4
CTTATAC	2360	0.0	17.637712	37
ACGGACC	2940	0.0	17.052721	8
GTATACG	120	1.04333594E-7	16.958332	1
GACGGAC	2995	0.0	16.801334	7
AGACGGA	3185	0.0	16.20565	6
CGGACCA	3145	0.0	16.058823	9
CGCAAGA	3185	0.0	15.973311	2
GCGCAAG	3145	0.0	15.941176	1
TCTATAC	440	0.0	15.556818	3
ATACCGT	1910	0.0	15.303664	6
AGAGCGA	3245	0.0	15.278891	15
GCAAGAC	3660	0.0	15.113387	3
TACCGTC	1905	0.0	15.052493	7
ACCGTCG	1930	0.0	14.953368	8
TCGTTTA	1910	0.0	14.91623	30
GAGCGAA	3350	0.0	14.855224	16
CAGAGCG	3400	0.0	14.8	14
TCTTATA	3795	0.0	14.673254	37
>>END_MODULE